Date published: 2025-9-5

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ERp57 抗体 (MaP.ERp57): sc-23886

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说明书
  • ERp57 抗体 MaP.ERp57 是小鼠单克隆 IgG1 κ,ERp57抗体, 在24篇文献中引用,规格为200 µg/ml
  • 针对重组human ERp57
  • ERp57 抗体 (MaP.ERp57) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 ERp57; 也和以下物种反应,包括: canine
  • 抗ERp57抗体(MaP.ERp57)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ERp57 (MaP.ERp57): sc-23886免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 ERp57 抗体 (MaP.ERp57) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ERp57 抗体 (MaP.ERp57) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

ERp57抗体(MaP.ERp57)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测小鼠、大鼠和人类来源的ERp57蛋白。抗ERp57抗体(MaP.ERp57)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。ERp57,也称为GRP57、GRP58、ERp60和ERp61,在主要组织相容性复合物(MHC)I类抗原呈递途径中起着至关重要的作用,该途径对于针对细胞内病原体的免疫反应至关重要。ERp57主要位于内质网中,与MHC I类分子相互作用,在结合与抗原处理相关的转运蛋白(TAP)之前促进正确的折叠和组装。这种相互作用对于向细胞毒性T淋巴细胞有效呈现细胞质来源的肽至关重要,最终导致靶向免疫反应。人类ERp57基因位于15q15染色体上,编码一种505个氨基酸的蛋白质,该蛋白质包含两个高度保守的Trp-Cys-Gly-His-Cys-Lys基序,这凸显了进化的重要性。此外,ERp57可能具有多种功能,可作为蛋白酶、蛋白二硫键异构酶或磷脂酶,这进一步突出了其在细胞过程和免疫调节中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ERp57 抗体 (MaP.ERp57) 参考文献:

  1. CD34 衍生物人类朗格汉斯细胞活化后,MHC I 类分子从晚期内体向细胞表面移动。  |  MacAry, PA., et al. 2001. Proc Natl Acad Sci U S A. 98: 3982-7. PMID: 11274420
  2. AGR2、ERp57/GRP58和其他一些人类蛋白质二硫键异构酶。  |  Shishkin, SS., et al. 2013. Biochemistry (Mosc). 78: 1415-30. PMID: 24490732
  3. ERp57在神经退行性疾病和再生中的作用。  |  Bargsted, L., et al. 2016. Neural Regen Res. 11: 232-3. PMID: 27073369
  4. 蛋白质二硫键异构酶:PDI和ERp57在神经系统中的新兴作用以及作为ALS的治疗靶点。  |  Perri, E., et al. 2017. Expert Opin Ther Targets. 21: 37-49. PMID: 27786579
  5. 多功能分子ERp57:从癌症到神经退行性疾病。  |  Hettinghouse, A., et al. 2018. Pharmacol Ther. 181: 34-48. PMID: 28723413
  6. ERp57在癌症中的作用。  |  Song, D., et al. 2021. J Cancer. 12: 2456-2464. PMID: 33758622
  7. ERp57伴侣蛋白可保护神经细胞免受A和β诱导的毒性。  |  Di Risola, D., et al. 2022. J Neurochem. 162: 322-336. PMID: 35699375
  8. 现成的CAR-NK细胞靶向免疫原性细胞死亡标记物ERp57,可发挥强大的抗肿瘤活性,并与ICD诱导剂奥沙利铂协同作用。  |  Zheng, L., et al. 2024. J Immunother Cancer. 12: PMID: 38964787
  9. 抑制ERp57可减少破骨细胞活性,并通过干预钙振荡和钙调蛋白/钙调神经磷酸酶/Nfatc1通路改善卵巢切除术引起的骨质流失。  |  Yuan, T., et al. 2024. Heliyon. 10: e35374. PMID: 39170388
  10. 人类葡萄糖调节蛋白 ERp57/GRP58 的分子克隆,它是一种硫醇依赖性还原酶。鉴定其分泌形式和致癌转化的诱导表达。  |  Hirano, N., et al. 1995. Eur J Biochem. 234: 336-42. PMID: 8529662
  11. 硫醇氧化还原酶 ERp57 是 MHC I 类肽加载复合体的一个组成部分。  |  Hughes, EA. and Cresswell, P. 1998. Curr Biol. 8: 709-12. PMID: 9637923
  12. 硫醇依赖性还原酶 ERp57 在 MHC I 类分子组装中的作用  |  Morrice, NA. and Powis, SJ. 1998. Curr Biol. 8: 713-6. PMID: 9637924

订购信息

产品名称产品编号规格价格数量收藏夹

ERp57 抗体 (MaP.ERp57)

sc-23886
200 µg/ml
$316.00

ERp57 (MaP.ERp57): m-IgG Fc BP-HRP 套装

sc-528379
200 µg Ab; 10 µg BP
$354.00

ERp57 (MaP.ERp57): m-IgGκ BP-HRP 套装

sc-520742
200 µg Ab, 40 µg BP
$354.00

ERp57 (MaP.ERp57): m-IgG1 BP-HRP 套装

sc-542906
200 µg Ab; 20 µg BP
$354.00

ERp57 抗体 (MaP.ERp57) AC

sc-23886 AC
500 µg/ml, 25% agarose
$416.00

ERp57 抗体 (MaP.ERp57) HRP

sc-23886 HRP
200 µg/ml
$316.00

ERp57 抗体 (MaP.ERp57) FITC

sc-23886 FITC
200 µg/ml
$330.00

ERp57 抗体 (MaP.ERp57) PE

sc-23886 PE
200 µg/ml
$343.00

ERp57 抗体 (MaP.ERp57) Alexa Fluor® 488

sc-23886 AF488
200 µg/ml
$357.00

ERp57 抗体 (MaP.ERp57) Alexa Fluor® 546

sc-23886 AF546
200 µg/ml
$357.00

ERp57 抗体 (MaP.ERp57) Alexa Fluor® 594

sc-23886 AF594
200 µg/ml
$357.00

ERp57 抗体 (MaP.ERp57) Alexa Fluor® 647

sc-23886 AF647
200 µg/ml
$357.00

ERp57 抗体 (MaP.ERp57) Alexa Fluor® 680

sc-23886 AF680
200 µg/ml
$357.00

ERp57 抗体 (MaP.ERp57) Alexa Fluor® 790

sc-23886 AF790
200 µg/ml
$357.00

I am using ERp57 (MaP.ERp57): sc-23886 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good stainingworks well analysis ERp57 in fixed mouse rain by IF. Antibody gives high signal and low background, Good staining.
Date published: 2017-08-26
Rated 5 out of 5 by from IHC analysisThe staining shows reliable on paraffin human skin tissue. Best results was performed for 1:100 antibody dilution , with the IHC staining kit from Dako.
Date published: 2017-07-29
Rated 5 out of 5 by from Very dark band produced in western blotWe used this antibody for western blot staining and got a nice western blot at the correct molecular weight in HeLa cells
Date published: 2017-01-27
Rated 5 out of 5 by from nice quality!Distinct band is detected using this product! Nice quality! Got satisfactory results!
Date published: 2016-11-21
Rated 5 out of 5 by from Robust positive signal detected in WB in HeLaRobust positive signal detected in WB in HeLa , Daudi , MDCK and Caki-1 whole cell lysates. -SCBT QC
Date published: 2015-06-02
Rated 5 out of 5 by from Published data for WB and IF in human CD34+ cellsPublished data for WB and IF in human CD34+ cells. -SCBT Publication Review
Date published: 2015-05-24
Rated 5 out of 5 by from Published data for WB in human epithelialPublished data for WB in human epithelial ovarian cancer cell line. -SCBT Publication Review
Date published: 2015-04-09
Rated 5 out of 5 by from Band of interest observed in WB in both nonBand of interest observed in WB in both non-transfected 293T and human ERp57 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-10
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ERp57 抗体 (MaP.ERp57) is rated 5.0 out of 5 by 13.
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