ERp57 Antibody (MaP.ERp57): sc-23886

ERp57 Antibody (MaP.ERp57) is a mouse monoclonal IgG1 kappa light chain antibody that detects ERp57 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-ERp57 antibody (MaP.ERp57) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERp57, also known as GRP57, GRP58, ERp60, and ERp61, plays a crucial role in the major histocompatibility complex (MHC) class I antigen presentation pathway, which is essential for the immune response against intracellular pathogens. Located primarily in the endoplasmic reticulum, ERp57 interacts with MHC class I molecules, facilitating proper folding and assembly before binding to the transporter associated with antigen processing (TAP). This interaction is vital for the effective presentation of cytoplasmic-derived peptides to cytotoxic T lymphocytes, ultimately leading to targeted immune responses. The human ERp57 gene is located on chromosome 15q15 and encodes a 505 amino acid protein that contains two highly conserved Trp-Cys-Gly-His-Cys-Lys motifs, underscoring evolutionary importance. Additionally, ERp57 may exhibit multifunctional properties, acting as a protease, a protein disulfide isomerase, or a phospholipase, which further highlights significance in cellular processes and immune regulation.

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ERp57 Antibody (MaP.ERp57) References:

1. Mobilization of MHC class I molecules from late endosomes to the cell surface following activation of CD34-derived human Langerhans cells.  |  MacAry, PA., et al. 2001. Proc Natl Acad Sci U S A. 98: 3982-7. PMID: 11274420
2. AGR2, ERp57/GRP58, and some other human protein disulfide isomerases.  |  Shishkin, SS., et al. 2013. Biochemistry (Mosc). 78: 1415-30. PMID: 24490732
3. ERp57 in neurodegeneration and regeneration.  |  Bargsted, L., et al. 2016. Neural Regen Res. 11: 232-3. PMID: 27073369
4. Protein Disulphide Isomerases: emerging roles of PDI and ERp57 in the nervous system and as therapeutic targets for ALS.  |  Perri, E., et al. 2017. Expert Opin Ther Targets. 21: 37-49. PMID: 27786579
5. Multifunctional molecule ERp57: From cancer to neurodegenerative diseases.  |  Hettinghouse, A., et al. 2018. Pharmacol Ther. 181: 34-48. PMID: 28723413
6. Insights into the role of ERp57 in cancer.  |  Song, D., et al. 2021. J Cancer. 12: 2456-2464. PMID: 33758622
7. ERp57 chaperon protein protects neuronal cells from Aβ-induced toxicity.  |  Di Risola, D., et al. 2022. J Neurochem. 162: 322-336. PMID: 35699375
8. Off-the-shelf CAR-NK cells targeting immunogenic cell death marker ERp57 execute robust antitumor activity and have a synergistic effect with ICD inducer oxaliplatin.  |  Zheng, L., et al. 2024. J Immunother Cancer. 12: PMID: 38964787
9. Suppressing ERp57 diminishes osteoclast activity and ameliorates ovariectomy-induced bone loss via the intervention in calcium oscillation and the calmodulin/calcineurin/Nfatc1 pathway.  |  Yuan, T., et al. 2024. Heliyon. 10: e35374. PMID: 39170388
10. Molecular cloning of the human glucose-regulated protein ERp57/GRP58, a thiol-dependent reductase. Identification of its secretory form and inducible expression by the oncogenic transformation.  |  Hirano, N., et al. 1995. Eur J Biochem. 234: 336-42. PMID: 8529662
11. The thiol oxidoreductase ERp57 is a component of the MHC class I peptide-loading complex.  |  Hughes, EA. and Cresswell, P. 1998. Curr Biol. 8: 709-12. PMID: 9637923
12. A role for the thiol-dependent reductase ERp57 in the assembly of MHC class I molecules.  |  Morrice, NA. and Powis, SJ. 1998. Curr Biol. 8: 713-6. PMID: 9637924

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERp57 Antibody (MaP.ERp57) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-ERp57 Antibody (MaP.ERp57) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERp57 Antibody (MaP.ERp57) Specifications

Alternate Product Name: PDIA3

Product Clone Name: MaP.ERp57

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: ERp57

Epitope Species: human

Product Reactivity/Detection: ERp57

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for ERp57 Antibody (MaP.ERp57): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin