Anti-ERp57 Antibody (MaP.ERp57) recommended for detection of ERp57 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-ERp57 Antibody (MaP.ERp57): sc-23886

Anti-ERp57 Antibody (MaP.ERp57) is rated 5.0 out of 5 by 13.
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Datasheets

    • Anti-ERp57 Antibody (MaP.ERp57) is a mouse monoclonal IgG1 κ ERp57 antibody, cited in 21 publications, provided at 200 µg/ml
    • raised against recombinant human ERp57
    • Anti-ERp57 Antibody (MaP.ERp57) is recommended for detection of ERp57 of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including canine
    • Anti-ERp57 Antibody (MaP.ERp57) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ERp57 (MaP.ERp57): sc-23886.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ERp57 Antibody (MaP.ERp57) for WB and IHC(P) applications. These reagents are now offered in bundles with ERp57 Antibody (MaP.ERp57) (see ordering information below).
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I am using ERp57 (MaP.ERp57): sc-23886 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good staining works well analysis ERp57 in fixed mouse rain by IF. Antibody gives high signal and low background, Good staining.
Date published: 2017-08-26
Rated 5 out of 5 by from IHC analysis The staining shows reliable on paraffin human skin tissue. Best results was performed for 1:100 antibody dilution , with the IHC staining kit from Dako.
Date published: 2017-07-29
Rated 5 out of 5 by from Very dark band produced in western blot We used this antibody for western blot staining and got a nice western blot at the correct molecular weight in HeLa cells
Date published: 2017-01-27
Rated 5 out of 5 by from nice quality! Distinct band is detected using this product! Nice quality! Got satisfactory results!
Date published: 2016-11-21
Rated 5 out of 5 by from Robust positive signal detected in WB in HeLa Robust positive signal detected in WB in HeLa , Daudi , MDCK and Caki-1 whole cell lysates. -SCBT QC
Date published: 2015-06-02
Rated 5 out of 5 by from Published data for WB and IF in human CD34+ cells Published data for WB and IF in human CD34+ cells. -SCBT Publication Review
Date published: 2015-05-24
Rated 5 out of 5 by from Published data for WB in human epithelial Published data for WB in human epithelial ovarian cancer cell line. -SCBT Publication Review
Date published: 2015-04-09
Rated 5 out of 5 by from Band of interest observed in WB in both non Band of interest observed in WB in both non-transfected 293T and human ERp57 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-10
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