ERK 5 Antibody (C-7): sc-398015

ERK 5 Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects ERK 5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-ERK 5 antibody (C-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ERK 5, a member of the mitogen-activated protein kinase (MAPK) family, plays a crucial role in cellular processes such as proliferation, differentiation, and survival, making ERK 5 essential for normal cellular function and response to external stimuli. ERK 5 is localized primarily in the nucleus, where ERK 5 regulates gene expression by phosphorylating transcription factors, thereby influencing various signaling pathways vital for cell growth and development. The unique structure of ERK 5, characterized by a long C-terminal extension compared to other ERK family members, allows for specific interactions with distinct substrates and regulatory proteins, further emphasizing ERK 5′s role in mediating cellular responses. Dual phosphorylation at specific threonine and tyrosine residues regulates ERK 5 activation, which is critical for enzymatic activity and downstream signaling. Understanding ERK 5 function and regulation is important for elucidating ERK 5′s involvement in various diseases, including cancer, where aberrant signaling pathways can lead to uncontrolled cell growth.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ERK 5 Antibody (C-7) References:

1. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ.  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
2. Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product.  |  Crews, CM. and Erikson, RL. 1992. Proc Natl Acad Sci U S A. 89: 8205-9. PMID: 1381507
3. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
4. Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase.  |  Boulton, TG., et al. 1991. Biochemistry. 30: 278-86. PMID: 1846291
5. Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase).  |  Payne, DM., et al. 1991. EMBO J. 10: 885-92. PMID: 1849075
6. ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
7. ERK5 and its role in tumour development.  |  Lochhead, PA., et al. 2012. Biochem Soc Trans. 40: 251-6. PMID: 22260700
8. MEKK3-MEK5-ERK5 signaling promotes mitochondrial degradation.  |  Craig, JE., et al. 2020. Cell Death Discov. 6: 107. PMID: 33101709
9. Aerobic Exercise Alters the Melanoma Microenvironment and Modulates ERK5 S496 Phosphorylation.  |  Savage, H., et al. 2023. Cancer Immunol Res. 11: 1168-1183. PMID: 37307577
10. Imidazopyridine Compounds as ERK5 Inhibitors for Treating Cancer.  |  Sabnis, RW. 2024. ACS Med Chem Lett. 15: 777-778. PMID: 38894904
11. Components of a new human protein kinase signal transduction pathway.  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
12. ERK6, a mitogen-activated protein kinase involved in C2C12 myoblast differentiation.  |  Lechner, C., et al. 1996. Proc Natl Acad Sci U S A. 93: 4355-9. PMID: 8633070

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERK 5 Antibody (C-7) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-ERK 5 Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERK 5 Antibody (C-7) Specifications

Alternate Product Name: ERK 5

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 671-698

Epitope Target: ERK 5

Epitope Species: human

Product Reactivity/Detection: ERK 5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for ERK 5 Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred p-ERK 5 antibody is p-ERK 5 Antibody (1.T218/Y220): sc-135760.