ERK 1 Antibody (G-8): sc-271269

ERK 1 Antibody (G-8) is a mouse monoclonal IgG2b kappa light chain antibody specific for an epitope mapping between amino acids 291-335 within subdomain XI of ERK 1 of rat origin, exhibiting species reactivity with mouse, rat, and human proteins by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA); ERK 1 (G-8) antibody is also reactive with additional species including equine, canine, bovine, and porcine. ERK1, also known as Mitogen-activated protein kinase 3 (MAPK3), is a crucial component of the MAPK/ERK signaling pathway, which transmits signals from the cell surface to the nucleus in response to various extracellular stimuli like growth factors, hormones, and cytokines. Post-translational modifications of ERK1, particularly phosphorylation, are essential for activation and function. ERK1 is activated by dual phosphorylation on threonine 202 and tyrosine 204 residues within the activation loop, mediated by upstream kinases MEK1 and MEK2. This phosphorylation induces conformational changes that enhance ERK1′s catalytic activity, enabling phosphorylation of a wide array of downstream substrates involved in cell proliferation, differentiation, and survival. ERK1 undergoes other post-translational modifications such as ubiquitination and acetylation, which can influence stability and interactions with other proteins. Understanding these modifications is important because dysregulation of ERK1 activity and post-translational modifications are implicated in various pathological conditions, including cancer and neurodegenerative diseases. Anti-ERK 1 antibody (G-8) is an invaluable tool for researchers investigating the role of ERK1 in signal transduction pathways and implications in health and disease.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ERK 1 Antibody (G-8) has 60 citations in a variety of scientific publications.

Primary antibodies like Anti-ERK 1 Antibody (G-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ERK 1 Antibody (G-8) Specifications

Alternate Product Name: MAPK3

Product Clone Name: G-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 291-335

Epitope Target: ERK 1

Epitope Species: rat

Product Reactivity/Detection: ERK 1 p44

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 60 citations in various scientific publications

Available Conjugates/Forms for ERK 1 Antibody (G-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred p-ERK antibody is p-ERK Antibody (E-4): sc-7383.