ENT2 Antibody (A-8): sc-373872

ENT2 Antibody (A-8) is a mouse monoclonal IgG2b antibody that detects Equilibrative Nucleoside Transporter 2 (ENT2) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ENT2 plays a crucial role in the equilibrative transport of nucleosides and hypoxanthine, which are vital for numerous cellular processes, including nucleotide synthesis and energy metabolism. ENT2 (A-8) antibody recognizes a protein with notable purine selectivity, effectively regulating the availability of purine nucleotides within cells, thereby influencing critical physiological functions such as cell signaling and proliferation. Additionally, ENT2′s ability to transport nucleoside analogs makes anti-ENT2 antibody (A-8) valuable for studying pharmacokinetics of antiviral and anticancer therapies, as ENT2 facilitates the uptake of these therapeutic agents into target cells. Understanding ENT2′s function and significance helps researchers explore its potential as a therapeutic target and implications in various diseases.

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ENT2 Antibody (A-8) References:

1. Distribution of mRNA encoding a nitrobenzylthioinosine-insensitive nucleoside transporter (ENT2) in rat brain.  |  Anderson, CM., et al. 1999. Brain Res Mol Brain Res. 70: 293-7. PMID: 10407178
2. Kinetic and pharmacological properties of cloned human equilibrative nucleoside transporters, ENT1 and ENT2, stably expressed in nucleoside transporter-deficient PK15 cells. Ent2 exhibits a low affinity for guanosine and cytidine but a high affinity for inosine.  |  Ward, JL., et al. 2000. J Biol Chem. 275: 8375-81. PMID: 10722669
3. Functional and molecular characterization of nucleobase transport by recombinant human and rat equilibrative nucleoside transporters 1 and 2. Chimeric constructs reveal a role for the ENT2 helix 5-6 region in nucleobase translocation.  |  Yao, SY., et al. 2002. J Biol Chem. 277: 24938-48. PMID: 12006583
4. Functional characterization and haplotype analysis of polymorphisms in the human equilibrative nucleoside transporter, ENT2.  |  Owen, RP., et al. 2006. Drug Metab Dispos. 34: 12-5. PMID: 16214850
5. Different signaling pathways utilized by insulin to regulate the expression of ENT2, CNT1, CNT2 nucleoside transporters in rat cardiac fibroblasts.  |  Podgorska, M., et al. 2007. Arch Biochem Biophys. 464: 344-9. PMID: 17537394
6. Hypoxanthine uptake and release by equilibrative nucleoside transporter 2 (ENT2) of rat microvascular endothelial cells.  |  Robillard, KR., et al. 2008. Microvasc Res. 75: 351-7. PMID: 18048066
7. Crystallographic analysis of the ENTH domain from yeast epsin Ent2 that induces a cell division phenotype.  |  Costakes, GT., et al. 2013. Protein Sci. 22: 755-61. PMID: 23553749
8. Localization and Expression of Nucleoside Transporters ENT1 and ENT2 in Polar Cells of Intestinal Epithelium.  |  Senyavina, NV., et al. 2016. Bull Exp Biol Med. 160: 771-4. PMID: 27160886
9. Treatment of restless legs syndrome/Willis-Ekbom disease with the non-selective ENT1/ENT2 inhibitor dipyridamole: testing the adenosine hypothesis.  |  Garcia-Borreguero, D., et al. 2018. Sleep Med. 45: 94-97. PMID: 29680437
10. PF-07321332 (Nirmatrelvir) does not interact with human ENT1 or ENT2: Implications for COVID-19 patients.  |  Hau, RK., et al. 2022. Clin Transl Sci. 15: 1599-1605. PMID: 35505633

The preferred ENT2 antibody is ENT2 Antibody (D-9): sc-373871.