ENDOG Antibody (C-1): sc-398683

ENDOG Antibody (C-1) is a mouse monoclonal IgG1 antibody that detects Endonuclease G (ENDOG) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ENDOG is a crucial nuclear-encoded protein that plays a significant role in mitochondrial function by localizing to the mitochondria, where anti-ENDOG antibody (C-1) exhibits sugar-nonspecific nuclease activity, primarily cleaving single-stranded DNA. This enzyme is synthesized as a propeptide with an amino-terminal presequence that directs ENDOG to the mitochondria, and upon apoptotic stimulation, ENDOG translocates to the nuclei, functioning as a nuclease without sequence specificity. Notably, ENDOG is involved in the generation of RNA primers necessary for mitochondrial DNA replication, as ENDOG possesses ribonuclease and RNase H activities, which are essential for the proper functioning of DNA polymerase gamma. ENDOG′s ability to cleave DNA at GC tracts is particularly important for maintaining mitochondrial DNA integrity during apoptosis, ensuring efficient DNA breakdown in apoptotic cells. ENDOG is located in the mitochondrial intermembrane space, highlighting a specialized role in mitochondrial dynamics, while mapping to chromosome 9q34.11 in humans underscores genetic significance. ENDOG monoclonal antibody (C-1) is an invaluable tool for researchers studying mitochondrial function, apoptosis, and the intricate mechanisms of DNA metabolism.

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ENDOG Antibody (C-1) References:

1. Sensitivity of human prostate cancer cells to chemotherapeutic drugs depends on EndoG expression regulated by promoter methylation.  |  Wang, X., et al. 2008. Cancer Lett. 270: 132-43. PMID: 18565644
2. EndoG links Bnip3-induced mitochondrial damage and caspase-independent DNA fragmentation in ischemic cardiomyocytes.  |  Zhang, J., et al. 2011. PLoS One. 6: e17998. PMID: 21437288
3. Caspase-3 feedback loop enhances Bid-induced AIF/endoG and Bak activation in Bax and p53-independent manner.  |  Guo, W., et al. 2015. Cell Death Dis. 6: e1919. PMID: 26469967
4. MGL induces nuclear translocation of EndoG and AIF in caspase-independent T cell death.  |  Bu, Q., et al. 2015. Cell Mol Biol Lett. 20: 816-24. PMID: 26556846
5. EndoG Knockout Mice Show Increased Brown Adipocyte Recruitment in White Adipose Tissue and Improved Glucose Homeostasis.  |  Pardo, R., et al. 2016. Endocrinology. 157: 3873-3887. PMID: 27547848
6. Apoptotic Endonuclease EndoG Inhibits Telomerase Activity and Induces Malignant Transformation of Human CD4+ T Cells.  |  Vasina, DA., et al. 2017. Biochemistry (Mosc). 82: 24-37. PMID: 28320284
7. Alternative splicing of telomerase catalytic subunit hTERT generated by apoptotic endonuclease EndoG induces human CD4+ T cell death.  |  Zhdanov, DD., et al. 2017. Eur J Cell Biol. 96: 653-664. PMID: 28886883
8. Intracellular Localization of Apoptotic Endonuclease EndoG and Splice-Variants of Telomerase Catalytic Subunit hTERT.  |  Zhdanov, DD., et al. 2017. Biochemistry (Mosc). 82: 894-905. PMID: 28941457
9. SIRT6 regulates obesity-induced oxidative stress via ENDOG/SOD2 signaling in the heart.  |  Gao, S., et al. 2023. Cell Biol Toxicol. 39: 1489-1507. PMID: 35798905
10. Metformin alleviates HFD-induced oxidative stress in hepatocyte via activating SIRT6/PGC-1α/ENDOG signaling.  |  Gao, S., et al. 2022. Clin Sci (Lond). 136: 1711-1730. PMID: 36315407

The preferred ENDOG antibody is ENDOG Antibody (B-2): sc-365359.