EMSY Antibody (E-9): sc-515469
- EMSY Antibody (E-9) is a mouse monoclonal IgG1 κ EMSY antibody provided at 200 µg/ml
- raised against amino acids 1-83 mapping at the N-terminus of EMSY of human origin
- EMSY Antibody (E-9) is recommended for detection of EMSY of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-EMSY Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EMSY Antibody (E-9) for WB applications. These reagents are now offered in bundles with EMSY Antibody (E-9) (see ordering information below).
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EMSY Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects EMSY protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-EMSY antibody (E-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EMSY plays a critical role in chromatin remodeling and interacts with BRCA2, a key player in DNA repair processes. This interaction has been confirmed in HeLa cells, highlighting EMSY′s importance in maintaining genomic stability. EMSY overexpression is strongly associated with amplification in sporadic breast cancer and higher-grade ovarian cancer, with EMSY gene being amplified in 18% of breast cancer cell lines. Such amplification indicates poor prognosis and links to shortened disease-free survival times. EMSY features a conserved 80 amino acid sequence at its N-terminus across various species, underscoring evolutionary significance. In studies involving irradiated mouse embryonic fibroblasts, EMSY (E-9) monoclonal antibody detected protein migration to sites of damaged DNA, emphasizing EMSY′s role in cellular responses to DNA damage.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EMSY Antibody (E-9) References:
- The BRCA2-EMSY connection: implications for breast and ovarian tumorigenesis. | Haber, DA. 2003. Cell. 115: 507-8. PMID: 14651841
- EMSY links the BRCA2 pathway to sporadic breast and ovarian cancer. | Hughes-Davies, L., et al. 2003. Cell. 115: 523-35. PMID: 14651845
- EMSY, a BRCA-2 partner in crime. | Livingston, DM. 2004. Nat Med. 10: 127-8. PMID: 14760417
- EMSY links breast cancer gene 2 to the 'Royal Family'. | Yao, J. and Polyak, K. 2004. Breast Cancer Res. 6: 201-3. PMID: 15318925
- Amplification of the BRCA2 pathway gene EMSY in sporadic breast cancer is related to negative outcome. | Rodriguez, C., et al. 2004. Clin Cancer Res. 10: 5785-91. PMID: 15355907
- Common variation in EMSY and risk of breast and ovarian cancer: a case-control study using HapMap tagging SNPs. | Benusiglio, PR., et al. 2005. BMC Cancer. 5: 81. PMID: 16029503
- Genomic instability of human mammary epithelial cells overexpressing a truncated form of EMSY. | Raouf, A., et al. 2005. J Natl Cancer Inst. 97: 1302-6. PMID: 16145051
- Amplification of EMSY, a novel oncogene on 11q13, in high grade ovarian surface epithelial carcinomas. | Brown, LA., et al. 2006. Gynecol Oncol. 100: 264-70. PMID: 16236351
- Crystal structure of the HP1-EMSY complex reveals an unusual mode of HP1 binding. | Huang, Y., et al. 2006. Structure. 14: 703-12. PMID: 16615912
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EMSY Antibody (E-9) | sc-515469 | 200 µg/ml | $316.00 | |||
EMSY Antibody (E-9): m-IgG Fc BP-HRP Bundle | sc-531384 | 200 µg Ab; 10 µg BP | $354.00 | |||
EMSY Antibody (E-9): m-IgGκ BP-HRP Bundle | sc-525060 | 200 µg Ab, 40 µg BP | $354.00 | |||
EMSY Antibody (E-9): m-IgG1 BP-HRP Bundle | sc-544518 | 200 µg Ab; 20 µg BP | $354.00 | |||
EMSY Antibody (E-9) AC | sc-515469 AC | 500 µg/ml, 25% agarose | $416.00 | |||
EMSY Antibody (E-9) HRP | sc-515469 HRP | 200 µg/ml | $316.00 | |||
EMSY Antibody (E-9) FITC | sc-515469 FITC | 200 µg/ml | $330.00 | |||
EMSY Antibody (E-9) PE | sc-515469 PE | 200 µg/ml | $343.00 | |||
EMSY Antibody (E-9) Alexa Fluor® 488 | sc-515469 AF488 | 200 µg/ml | $357.00 | |||
EMSY Antibody (E-9) Alexa Fluor® 546 | sc-515469 AF546 | 200 µg/ml | $357.00 | |||
EMSY Antibody (E-9) Alexa Fluor® 594 | sc-515469 AF594 | 200 µg/ml | $357.00 | |||
EMSY Antibody (E-9) Alexa Fluor® 647 | sc-515469 AF647 | 200 µg/ml | $357.00 | |||
EMSY Antibody (E-9) Alexa Fluor® 680 | sc-515469 AF680 | 200 µg/ml | $357.00 | |||
EMSY Antibody (E-9) Alexa Fluor® 790 | sc-515469 AF790 | 200 µg/ml | $357.00 |