EML1 Antibody (B-3): sc-390841

EML1 Antibody (B-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects EML1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EML1 (B-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EML1, also known as echinoderm microtubule-associated protein-like 1, plays a crucial role in regulating microtubule dynamics, which is essential for various cellular processes such as cell division, intracellular transport, and maintaining cell shape. EML1 presence in most tissues underscores importance in cellular function, although notably absent in thymus and peripheral blood lymphocytes. Human EML1 contains a WD40 domain, a structural motif that facilitates interactions with other proteins, thereby participating in critical processes like signal transduction and cytoskeleton assembly. EML1 has been implicated as a candidate gene for Usher syndrome type 1A, a genetic disorder characterized by hearing loss and vision impairment, highlighting significance in both normal physiology and disease states.

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EML1 Antibody (B-3) References:

1. Conservation of the WD-repeat, microtubule-binding protein, EMAP, in sea urchins, humans, and the nematode C. elegans.  |  Suprenant, KA., et al. 2000. Dev Genes Evol. 210: 2-10. PMID: 10603080
2. Saturable binding of the echinoderm microtubule-associated protein (EMAP) on microtubules, but not filamentous actin or vimentin filaments.  |  Eichenmüller, B., et al. 2001. Cell Motil Cytoskeleton. 50: 161-72. PMID: 11807937
3. Fusion of EML1 to ABL1 in T-cell acute lymphoblastic leukemia with cryptic t(9;14)(q34;q32).  |  De Keersmaecker, K., et al. 2005. Blood. 105: 4849-52. PMID: 15713800
4. EML1-associated brain overgrowth syndrome with ribbon-like heterotopia.  |  Oegema, R., et al. 2019. Am J Med Genet C Semin Med Genet. 181: 627-637. PMID: 31710781
5. Disruption in murine Eml1 perturbs retinal lamination during early development.  |  Collin, GB., et al. 2020. Sci Rep. 10: 5647. PMID: 32221352
6. EML1 is essential for retinal photoreceptor migration and survival.  |  Poria, D., et al. 2022. Sci Rep. 12: 2897. PMID: 35190581
7. Human cerebral organoids reveal progenitor pathology in EML1-linked cortical malformation.  |  Jabali, A., et al. 2022. EMBO Rep. 23: e54027. PMID: 35289477
8. Eml1 promotes axonal growth by enhancing αTAT1-mediated microtubule acetylation.  |  Zhang, Y., et al. 2024. Biochim Biophys Acta Mol Cell Res. 1871: 119770. PMID: 38897390
9. Forebrain Eml1 depletion reveals early centrosomal dysfunction causing subcortical heterotopia.  |  Zaidi, D., et al. 2024. J Cell Biol. 223: PMID: 39316454
10. Molecular characterization of the 77-kDa echinoderm microtubule-associated protein. Homology to the beta-transducin family.  |  Li, Q. and Suprenant, KA. 1994. J Biol Chem. 269: 31777-84. PMID: 7989351
11. Isolation of a novel human homologue of the gene coding for echinoderm microtubule-associated protein (EMAP) from the Usher syndrome type 1a locus at 14q32.  |  Eudy, JD., et al. 1997. Genomics. 43: 104-6. PMID: 9226380
12. Overexpression of the 77-kD echinoderm microtubule-associated protein (EMAP), a WD-40 repeat protein, in baculovirus-infected Sf9 cells.  |  Daggett, MA., et al. 1998. Cell Motil Cytoskeleton. 41: 57-67. PMID: 9744299

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EML1 Antibody (B-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-EML1 Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EML1 Antibody (B-3) Specifications

Alternate Product Name: EML1

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 60-120

Epitope Target: EML1

Epitope Species: human

Product Reactivity/Detection: EML1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for EML1 Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred EML2 antibody is EML2 Antibody (F-3): sc-374627.