EMBP Antibody (F-6): sc-365701

EMBP Antibody (F-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects EMBP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). EMBP Antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The eosinophil major basic protein (EMBP), also known as MBP, PRG2, proteoglycan 2, BMPG, or bone marrow natural killer cell activator, is a critical component of the crystalline core of eosinophil granules, which are essential for the immune response, particularly in combating parasitic infections. EMBP is predominantly located in eosinophils, where EMBP plays a vital role in mediating cytotoxic effects against helminths and modulating immune hypersensitivity reactions. EMBP forms complexes with proteins such as pregnancy-associated plasma protein A, angiotensinogen, and C3dg in high concentrations in the placenta and pregnancy serum, underscoring EMBP′s importance in reproductive immunology. EMBP activates phosphoinositide 3-kinase and protein kinase C ζ signaling pathways in neutrophils, highlighting EMBP′s role in immune cell signaling and function. EMBP transcription is regulated by transcription factors including GATA-1, PU.1, and C/EBPε isoforms, adding complexity to EMBP′s regulation and function in immune responses.

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EMBP Antibody (F-6) References:

1. Eosinophil major basic protein-1 does not contribute to allergen-induced airway pathologies in mouse models of asthma.  |  Denzler, KL., et al. 2000. J Immunol. 165: 5509-17. PMID: 11067904
2. A novel human homolog of eosinophil major basic protein.  |  Plager, DA., et al. 2001. Immunol Rev. 179: 192-202. PMID: 11292023
3. Eosinophil major basic protein: first identified natural heparanase-inhibiting protein.  |  Temkin, V., et al. 2004. J Allergy Clin Immunol. 113: 703-9. PMID: 15100677
4. Human eosinophil major basic protein 2: location of disulfide bonds and free sulfhydryl groups.  |  Wagner, LA., et al. 2007. Protein J. 26: 13-8. PMID: 17136616
5. Mechanisms of eosinophil major basic protein-induced hyperexcitability of vagal pulmonary chemosensitive neurons.  |  Gu, Q., et al. 2009. Am J Physiol Lung Cell Mol Physiol. 296: L453-61. PMID: 19136577
6. Eosinophil major basic protein activates human cord blood mast cells primed with fibroblast membranes by integrin-β1.  |  Ben-Zimra, M., et al. 2013. Allergy. 68: 1259-68. PMID: 24112102
7. Antibacterial properties of eosinophil major basic protein and eosinophil cationic protein.  |  Lehrer, RI., et al. 1989. J Immunol. 142: 4428-34. PMID: 2656865
8. Human eosinophil major basic protein causes hyperreactivity of respiratory smooth muscle. Role of the epithelium.  |  Flavahan, NA., et al. 1988. Am Rev Respir Dis. 138: 685-8. PMID: 3202421
9. Elevated levels of eosinophil major basic protein in the sera of patients with systemic sclerosis.  |  Cox, D., et al. 1995. Arthritis Rheum. 38: 939-45. PMID: 7612043
10. Human eosinophil major basic protein is an endogenous allosteric antagonist at the inhibitory muscarinic M2 receptor.  |  Jacoby, DB., et al. 1993. J Clin Invest. 91: 1314-8. PMID: 8473484

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EMBP Antibody (F-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-EMBP Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EMBP Antibody (F-6) Specifications

Alternate Product Name: EMBP

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-222

Epitope Target: EMBP

Epitope Species: human

Product Reactivity/Detection: EMBP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for EMBP Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred EMBP antibody is EMBP Antibody (BMK13): sc-59164.