EMBP Antibody (BMK13): sc-59164

EMBP Antibody (BMK13) is a mouse monoclonal IgG1 kappa light chain antibody that detects the eosinophil major basic protein (EMBP) of human origin by western blotting (WB), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-EMBP antibody (BMK13) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The eosinophil major basic protein, also known as MBP, PRG2, proteoglycan 2, BMPG, or bone marrow natural killer cell activator, is a critical component of the crystalline core of eosinophil granules, playing a significant role in the immune response. EMBP is primarily located in eosinophils, which are white blood cells involved in combating parasitic infections and mediating allergic reactions. The presence of EMBP in eosinophils is crucial for their cytotoxic activity against helminths and contributes to the pathophysiology of various allergic diseases. Additionally, EMBP is known to interact with other proteins, such as pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg, particularly during pregnancy, where it forms complexes that may influence immune responses. The regulation of EMBP transcription involves intricate interactions among transcription factors such as GATA-1, PU.1, and C/EBPε isoforms, highlighting its importance in both normal physiology and disease states.

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EMBP Antibody (BMK13) References:

1. C/EBPbeta and GATA-1 synergistically regulate activity of the eosinophil granule major basic protein promoter: implication for C/EBPbeta activity in eosinophil gene expression.  |  Yamaguchi, Y., et al. 1999. Blood. 94: 1429-39. PMID: 10438731
2. Expression of recombinant human pregnancy-associated plasma protein-A and identification of the proform of eosinophil major basic protein as its physiological inhibitor.  |  Overgaard, MT., et al. 2000. J Biol Chem. 275: 31128-33. PMID: 10913121
3. Crystal structure of the eosinophil major basic protein at 1.8 A. An atypical lectin with a paradigm shift in specificity.  |  Swaminathan, GJ., et al. 2001. J Biol Chem. 276: 26197-203. PMID: 11319227
4. Eosinophil major basic protein: first identified natural heparanase-inhibiting protein.  |  Temkin, V., et al. 2004. J Allergy Clin Immunol. 113: 703-9. PMID: 15100677
5. Eosinophil-granule major basic protein, a C-type lectin, binds heparin.  |  Swaminathan, GJ., et al. 2005. Biochemistry. 44: 14152-8. PMID: 16245931
6. Localization of eosinophil granule major basic protein in human basophils.  |  Ackerman, SJ., et al. 1983. J Exp Med. 158: 946-61. PMID: 6350526
7. Human eosinophil major basic protein, a mediator of allergic inflammation, is expressed by alternative splicing from two promoters.  |  Li, MS., et al. 1995. Biochem J. 305 (Pt 3): 921-7. PMID: 7531438
8. Expression, purification, and characterization of the recombinant proform of eosinophil granule major basic protein.  |  Popken-Harris, P., et al. 1995. J Immunol. 155: 1472-80. PMID: 7543523
9. The identification and cloning of a murine major basic protein gene expressed in eosinophils.  |  Larson, KA., et al. 1995. J Immunol. 155: 3002-12. PMID: 7673718
10. Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein.  |  Oxvig, C., et al. 1993. J Biol Chem. 268: 12243-6. PMID: 7685339
11. CD4 T suppressor cells mediate interferon tau protection against experimental allergic encephalomyelitis.  |  Mujtaba, MG., et al. 1997. J Neuroimmunol. 75: 35-42. PMID: 9143235
12. Elevated serum levels of eosinophil major basic protein in patients with myeloproliferative disorders without eosinophilia.  |  Mukai, HY., et al. 1997. Int J Hematol. 66: 197-202. PMID: 9277050

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EMBP Antibody (BMK13) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-EMBP Antibody (BMK13) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EMBP Antibody (BMK13) Specifications

Alternate Product Name: EMBP

Product Clone Name: BMK13

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: EMBP

Epitope Species: human

Product Reactivity/Detection: EMBP irrespective of the stages of eosinophil activation

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for EMBP Antibody (BMK13): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin