Egr-2 Antibody (1G5): sc-293195

Egr-2 Antibody (1G5) is a mouse monoclonal IgG2b kappa light chain antibody that detects the Egr-2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-Egr-2 antibody (1G5) is available as the non-conjugated form. Egr-2 protein plays a crucial role as a sequence-specific DNA-binding transcription factor, primarily involved in regulating gene expression during cellular growth and differentiation. Egr-2 binds to specific DNA sites in the promoter region of target genes, such as HoxA4, and is predominantly localized in the nucleus, where Egr-2 influences developmental processes. Egr-2′s importance is underscored by associations with congenital hypomyelination neuropathy (CHN) and Dejerine-Sottas syndrome (DSS), both characterized by severe neurological deficits and slow nerve conduction velocities due to mutations in the EGR2 gene. A high degree of conservation in the zinc finger region between human Egr-2 and mouse Egr-1, with 92% identity, highlights the evolutionary significance of Egr-2 in transcriptional regulation. Egr-2 monoclonal antibody (1G5) is an essential tool for researchers studying the molecular mechanisms underlying these neuropathies and the broader implications of Egr proteins in cellular function.

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Egr-2 Antibody (1G5) References:

1. Novel missense mutation in the early growth response 2 gene associated with Dejerine-Sottas syndrome phenotype.  |  Timmerman, V., et al. 1999. Neurology. 52: 1827-32. PMID: 10371530
2. Growth and gene expression profile analyses of endometrial cancer cells expressing exogenous PTEN.  |  Matsushima-Nishiu, M., et al. 2001. Cancer Res. 61: 3741-9. PMID: 11325847
3. EGR2 mutations in inherited neuropathies dominant-negatively inhibit myelin gene expression.  |  Nagarajan, R., et al. 2001. Neuron. 30: 355-68. PMID: 11394999
4. Growth-suppressive effects of BPOZ and EGR2, two genes involved in the PTEN signaling pathway.  |  Unoki, M. and Nakamura, Y. 2001. Oncogene. 20: 4457-65. PMID: 11494141
5. EGR2 mutation R359W causes a spectrum of Dejerine-Sottas neuropathy.  |  Boerkoel, CF., et al. 2001. Neurogenetics. 3: 153-7. PMID: 11523566
6. Mutation of a putative protein degradation gene LITAF/SIMPLE in Charcot-Marie-Tooth disease 1C.  |  Street, VA., et al. 2003. Neurology. 60: 22-6. PMID: 12525712
7. Egr-2 and Egr-3 are negative regulators of T cell activation.  |  Safford, M., et al. 2005. Nat Immunol. 6: 472-80. PMID: 15834410
8. Two missense mutations of EGR2 R359W and GJB1 V136A in a Charcot-Marie-Tooth disease family.  |  Chung, KW., et al. 2005. Neurogenetics. 6: 159-63. PMID: 15947997
9. Structure, chromosome location, and expression of the mouse zinc finger gene Krox-20: multiple gene products and coregulation with the proto-oncogene c-fos.  |  Chavrier, P., et al. 1989. Mol Cell Biol. 9: 787-97. PMID: 2496302
10. Molecular cloning, sequencing, and mapping of EGR2, a human early growth response gene encoding a protein with 'zinc-binding finger' structure.  |  Joseph, LJ., et al. 1988. Proc Natl Acad Sci U S A. 85: 7164-8. PMID: 3140236
11. Type I Interferon Signaling via the EGR2 Transcriptional Regulator Potentiates CAR T Cell-Intrinsic Dysfunction.  |  Jung, IY., et al. 2023. Cancer Discov. 13: 1636-1655. PMID: 37011008
12. Transcription factor EGR2 controls homing and pathogenicity of TH17 cells in the central nervous system.  |  Gao, Y., et al. 2023. Nat Immunol. 24: 1331-1344. PMID: 37443284

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Egr-2 Antibody (1G5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Egr-2 Antibody (1G5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Egr-2 Antibody (1G5) Specifications

Alternate Product Name: Egr-2

Product Clone Name: 1G5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 217-293

Epitope Target: Egr-2

Epitope Species: human

Product Reactivity/Detection: Egr-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Egr-2 Antibody (1G5): N/A

The preferred Egr-2 antibody is Egr-2 Antibody (G-9): sc-518117.