EF-1 ε1 Antibody (E-4): sc-376019

EF-1 ε1 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects EF-1 epsilon 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). EF-1 ε1 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EF-1 epsilon 1 protein, also known as Eukaryotic translation elongation factor 1 epsilon-1 or Multisynthetase complex auxiliary component p18, is a crucial 174 amino acid protein that plays a significant role in the translation process by facilitating the delivery of aminoacyl-tRNAs to the ribosome, thus promoting mRNA elongation. EF-1 epsilon 1 is part of a multi-protein complex that includes α, β, γ, and δ subunits, which work synergistically to ensure efficient protein synthesis. EF-1 epsilon 1 interacts specifically with Methionyl-tRNA synthetase, forming a macromolecular tRNA synthetase complex that is essential for the accurate ligation of amino acids to their corresponding tRNAs. EF-1 epsilon 1 has been implicated in cellular responses to DNA damage; upon such stress, EF-1 epsilon 1 translocates to the nucleus and interacts with ATM and ATR kinases, leading to the activation of the tumor suppressor protein p53. Loss of EF-1 epsilon 1 in murine models has been associated with increased susceptibility to spontaneous tumors, highlighting EF-1 epsilon 1′s potential role as a tumor suppressor and importance in maintaining genomic integrity.

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EF-1 ε1 Antibody (E-4) References:

1. The haploinsufficient tumor suppressor p18 upregulates p53 via interactions with ATM/ATR.  |  Park, BJ., et al. 2005. Cell. 120: 209-21. PMID: 15680327
2. AIMP3 haploinsufficiency disrupts oncogene-induced p53 activation and genomic stability.  |  Park, BJ., et al. 2006. Cancer Res. 66: 6913-8. PMID: 16849534
3. Quantitative evaluation and selection of reference genes in mouse oocytes and embryos cultured in vivo and in vitro.  |  Mamo, S., et al. 2007. BMC Dev Biol. 7: 14. PMID: 17341302
4. Supplementation of healthy volunteers with nutritionally relevant amounts of selenium increases the expression of lymphocyte protein biosynthesis genes.  |  Pagmantidis, V., et al. 2008. Am J Clin Nutr. 87: 181-9. PMID: 18175754
5. Determination of three-dimensional structure and residues of the novel tumor suppressor AIMP3/p18 required for the interaction with ATM.  |  Kim, KJ., et al. 2008. J Biol Chem. 283: 14032-40. PMID: 18343821
6. Absence of somatic mutation of a tumor suppressor gene eukaryotic translation elongation factor 1, epsilon-1 (EEF1E1), in common human cancers.  |  Kim, MS., et al. 2008. APMIS. 116: 832-3. PMID: 19024604
7. The p18 component of the multisynthetase complex shares a protein motif with the beta and gamma subunits of eukaryotic elongation factor 1.  |  Quevillon, S. and Mirande, M. 1996. FEBS Lett. 395: 63-7. PMID: 8849690
8. Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning.  |  Mao, M., et al. 1998. Proc Natl Acad Sci U S A. 95: 8175-80. PMID: 9653160

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EF-1 ε1 Antibody (E-4) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-EF-1 ε1 Antibody (E-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EF-1 ε1 Antibody (E-4) Specifications

Alternate Product Name: EF-1 ε1

Product Clone Name: E-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3-37

Epitope Target: EF-1 ε1

Epitope Species: human

Product Reactivity/Detection: EF-1 ε1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for EF-1 ε1 Antibody (E-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin