EED Antibody (B-3): sc-518116
- EED Antibody (B-3) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 36-58 near the N-terminus of EED of human origin
- recommended for detection of EED of human origin by WB, IP, IF and ELISA
- Anti-EED Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for EED Antibody (B-3) for WB applications. These reagents are now offered in bundles with EED Antibody (B-3) (see ordering information below).
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SEE ALSO...
EED Antibody (B-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects EED of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EED Antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EED, or embryonic ectoderm development protein, plays a crucial role in gene expression regulation through involvement in Polycomb-group (PcG) complexes, which maintain transcriptional repression of target genes across cell divisions. This function is vital for cellular memory and stable inheritance of gene activity during development. EED is highly conserved, with human EED showing 99.5% identity to mouse EED, and features seven WD repeats that facilitate protein-protein interactions. EED interacts specifically with histone deacetylase (HDAC) proteins, key players in chromatin remodeling and gene silencing. Two human EED transcripts produce isoforms of 535 and 494 amino acids, highlighting EED′s complexity and regulatory potential in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
EED Antibody (B-3) References:
- Transcriptional repression mediated by the human polycomb-group protein EED involves histone deacetylation. | van der Vlag, J. and Otte, AP. 1999. Nat Genet. 23: 474-8. PMID: 10581039
- Imprinted X inactivation maintained by a mouse Polycomb group gene. | Wang, J., et al. 2001. Nat Genet. 28: 371-5. PMID: 11479595
- Split luciferase-based biosensors for characterizing EED binders. | Li, L., et al. 2017. Anal Biochem. 522: 37-45. PMID: 28111304
- EED orchestration of heart maturation through interaction with HDACs is H3K27me3-independent. | Ai, S., et al. 2017. Elife. 6: PMID: 28394251
- Loss of maternal EED results in postnatal overgrowth. | Prokopuk, L., et al. 2018. Clin Epigenetics. 10: 95. PMID: 30005706
- EED-Targeted PROTACs Degrade EED, EZH2, and SUZ12 in the PRC2 Complex. | Hsu, JH., et al. 2020. Cell Chem Biol. 27: 41-46.e17. PMID: 31786184
- Identification of SET/EED dual binders as innovative PRC2 inhibitors. | Catalano, R., et al. 2022. Future Med Chem. 14: 609-621. PMID: 35348013
- EED is required for mouse primordial germ cell differentiation in the embryonic gonad. | Lowe, MG., et al. 2022. Dev Cell. 57: 1482-1495.e5. PMID: 35679863
- Gene-repressing epigenetic reader EED unexpectedly enhances cyclinD1 gene activation. | Zhang, M., et al. 2023. Mol Ther Nucleic Acids. 31: 717-729. PMID: 36923952
- Characterization of interactions between the mammalian polycomb-group proteins Enx1/EZH2 and EED suggests the existence of different mammalian polycomb-group protein complexes. | Sewalt, RG., et al. 1998. Mol Cell Biol. 18: 3586-95. PMID: 9584199
- Point mutations in the WD40 domain of Eed block its interaction with Ezh2. | Denisenko, O., et al. 1998. Mol Cell Biol. 18: 5634-42. PMID: 9742080
- HEED, the product of the human homolog of the murine eed gene, binds to the matrix protein of HIV-1. | Peytavi, R., et al. 1999. J Biol Chem. 274: 1635-45. PMID: 9880543
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EED Antibody (B-3) | sc-518116 | 200 µg/ml | $316.00 | |||
EED Antibody (B-3): m-IgG Fc BP-HRP Bundle | sc-531621 | 200 µg Ab; 10 µg BP | $354.00 | |||
EED Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-525381 | 200 µg Ab, 40 µg BP | $354.00 | |||
EED Antibody (B-3): m-IgG2b BP-HRP Bundle | sc-550300 | 200 µg Ab; 10 µg BP | $354.00 | |||
EED Antibody (B-3) AC | sc-518116 AC | 500 µg/ml, 25% agarose | $416.00 | |||
EED Antibody (B-3) HRP | sc-518116 HRP | 200 µg/ml | $316.00 | |||
EED Antibody (B-3) FITC | sc-518116 FITC | 200 µg/ml | $330.00 | |||
EED Antibody (B-3) PE | sc-518116 PE | 200 µg/ml | $343.00 | |||
EED Antibody (B-3) Alexa Fluor® 488 | sc-518116 AF488 | 200 µg/ml | $357.00 | |||
EED Antibody (B-3) Alexa Fluor® 546 | sc-518116 AF546 | 200 µg/ml | $357.00 | |||
EED Antibody (B-3) Alexa Fluor® 594 | sc-518116 AF594 | 200 µg/ml | $357.00 | |||
EED Antibody (B-3) Alexa Fluor® 647 | sc-518116 AF647 | 200 µg/ml | $357.00 | |||
EED Antibody (B-3) Alexa Fluor® 680 | sc-518116 AF680 | 200 µg/ml | $357.00 | |||
EED Antibody (B-3) Alexa Fluor® 790 | sc-518116 AF790 | 200 µg/ml | $357.00 |