EEA1 Antibody (E-8): sc-365652

EEA1 Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects EEA1 in mouse, rat, human, and monkey samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). EEA1 Antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EEA1, or early endosome antigen 1, plays a crucial role in the endocytic pathway by facilitating the fusion of early endosomes, which are essential for sorting and transporting endocytosed proteins. EEA1 is primarily located in the cytoplasm, where EEA1 co-localizes with the transferrin receptor and Rab5, highlighting EEA1′s importance in membrane trafficking and protein sorting. EEA1 contains specific structural motifs, such as a calmodulin-binding IQ motif and a cysteine-rich finger motif, which are vital for EEA1′s localization to early endosomes. Additionally, EEA1 shares sequence homology with several yeast proteins involved in membrane trafficking, including Vps27, Fab1, and Vac1, suggesting evolutionary conservation of EEA1′s function. The regulatory effects of 3′-phosphoinositides on membrane trafficking further underscore the significance of EEA1 in cellular processes, making anti-EEA1 antibody (E-8) an invaluable tool for researchers studying endosomal dynamics and protein transport mechanisms.

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EEA1 Antibody (E-8) References:

1. The small GTPase Rab22 interacts with EEA1 and controls endosomal membrane trafficking.  |  Kauppi, M., et al. 2002. J Cell Sci. 115: 899-911. PMID: 11870209
2. Induction of p38 mitogen-activated protein kinase reduces early endosome autoantigen 1 (EEA1) recruitment to phagosomal membranes.  |  Fratti, RA., et al. 2003. J Biol Chem. 278: 46961-7. PMID: 12963735
3. Molecular characterization of VAC1, a gene required for vacuole inheritance and vacuole protein sorting.  |  Weisman, LS. and Wickner, W. 1992. J Biol Chem. 267: 618-23. PMID: 1730622
4. Early Endosome Antigen 1 (EEA1) decreases in macrophages infected with Paracoccidioides brasiliensis.  |  Voltan, AR., et al. 2013. Med Mycol. 51: 759-64. PMID: 23566224
5. Endosomal Escape of Antisense Oligonucleotides Internalized by Stabilin Receptors Is Regulated by Rab5C and EEA1 During Endosomal Maturation.  |  Miller, CM., et al. 2018. Nucleic Acid Ther. 28: 86-96. PMID: 29437530
6. VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae.  |  Piper, RC., et al. 1995. J Cell Biol. 131: 603-17. PMID: 7593183
7. Novel PI(4)P 5-kinase homologue, Fab1p, essential for normal vacuole function and morphology in yeast.  |  Yamamoto, A., et al. 1995. Mol Biol Cell. 6: 525-39. PMID: 7663021
8. EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine 'fingers' and contains a calmodulin-binding IQ motif.  |  Mu, FT., et al. 1995. J Biol Chem. 270: 13503-11. PMID: 7768953
9. Endosomal localization of the autoantigen EEA1 is mediated by a zinc-binding FYVE finger.  |  Stenmark, H., et al. 1996. J Biol Chem. 271: 24048-54. PMID: 8798641
10. Rab4 and Rab7 define distinct nonoverlapping endosomal compartments.  |  Bottger, G., et al. 1996. J Biol Chem. 271: 29191-7. PMID: 8910576
11. Microtubules are involved in bafilomycin A1-induced tubulation and Rab5-dependent vacuolation of early endosomes.  |  D'Arrigo, A., et al. 1997. Eur J Cell Biol. 72: 95-103. PMID: 9157016
12. Identification of an early endosomal protein regulated by phosphatidylinositol 3-kinase.  |  Patki, V., et al. 1997. Proc Natl Acad Sci U S A. 94: 7326-30. PMID: 9207090

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EEA1 Antibody (E-8) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-EEA1 Antibody (E-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EEA1 Antibody (E-8) Specifications

Alternate Product Name: EEA1

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-27

Epitope Target: EEA1

Epitope Species: human

Product Reactivity/Detection: EEA1

Species Reactivity/Detection: mouse, rat, human, monkey

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for EEA1 Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin