EEA1 Antibody (E-8) recommended for detection of EEA1 of mouse, rat, human and monkey origin by WB, IP, IF, IHC(P) and ELISA

EEA1 Antibody (E-8): sc-365652

EEA1 Antibody (E-8) is rated 5.0 out of 5 by 9.
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Datasheets

    • EEA1 Antibody (E-8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 2-27 at the N-terminus of EEA1 of human origin
    • Anti-EEA1 Antibody (E-8) is recommended for detection of EEA1 of mouse, rat, human and monkey origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including porcine
    • Anti-EEA1 Antibody (E-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EEA1 (E-8): sc-365652.

How is the G-4 different from the E-8 antibody?

Asked by: racsumbr
Thank you for your question. These two mouse monoclonal antibodies are raised against different EEA1 antigens and have distinct epitopes. EEA1 Antibody (G-4): sc-137130 is raised against a recombinant protein antigen corresponding to amino acids 1-300 of EEA1 of human origin. And EEA1 Antibody (E-8): sc-365652 is raised against a peptide mapping between amino acids 2-27 of EEA1 of human origin.
Answered by: Technical Support
Date published: 2018-12-14

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-20
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Rated 5 out of 5 by from WB HEK293T細胞のWBを行い、1:1000希釈ではっきりした単一のバンドを予想された高さに認めました。
Date published: 2018-09-22
Rated 5 out of 5 by from 免疫染色 固定した繊維芽細胞の免疫染色に使用しました。通常通り1:400に希釈して使用して強い染色性が得られました。
Date published: 2018-03-22
Rated 5 out of 5 by from EEA1 EEA1 works well in immunocytochemistry at dilution1:600 in cultured methanol-fixed epidermal keratinocytes
Date published: 2018-01-24
Rated 5 out of 5 by from GOOD Ab! i tried with hippocampal culture, very good Ab for WB!
Date published: 2017-11-22
Rated 5 out of 5 by from Great product Great product for both western blot and IHC staining
Date published: 2017-02-05
Rated 5 out of 5 by from Popular antibody! Great Western blots, excellent IHC results. Reliable and consistent, this is a great choice for EEA1 detection.
Date published: 2016-12-30
Rated 5 out of 5 by from Robust signal in WB in A Robust signal in WB in A-431, NIH/3T3, Jurkat, C6 and COS whole cell lysates. -SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human duodenum tissue. -SCBT QC
Date published: 2015-01-08
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