EAPP Antibody (1E4): sc-130357

EAPP Antibody (1E4) is a mouse monoclonal IgG antibody that detects EAPP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). EAPP, or E2F-associated phosphoprotein, is a highly phosphorylated nuclear protein consisting of 285 amino acids, playing a crucial role in regulating E2F transcription factors, which are integral to apoptosis and cell proliferation. By interacting specifically with E2F-1, E2F-2, and E2F-3, EAPP modulates their activity by binding to their N-terminal domains, thereby preventing the association of essential cell cycle regulators like cyclin A and transcription factors such as Sp1, EBP1, and EBP2. This regulatory mechanism is vital for maintaining proper cell cycle progression, as EAPP is expressed throughout the cell cycle but is notably absent during mitosis, indicating its importance in facilitating the transition between cell cycle phases. EAPP is widely expressed across various tissues, with particularly high levels found in the placenta, pancreas, skeletal muscle, and heart, underscoring its significance in cellular functions and potential implications in cancer biology, where E2F activity is often dysregulated. Anti-EAPP antibody (1E4) is an essential tool for researchers investigating the intricate roles of E2F transcription factors and their associated pathways in health and disease.

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EAPP Antibody (1E4) References:

1. EAPP, a novel E2F binding protein that modulates E2F-dependent transcription.  |  Novy, M., et al. 2005. Mol Biol Cell. 16: 2181-90. PMID: 15716352
2. Transcription factor E2F-associated phosphoprotein (EAPP), RAM2/CDCA7L/JPO2 (R1), and simian virus 40 promoter factor 1 (Sp1) cooperatively regulate glucocorticoid activation of monoamine oxidase B.  |  Chen, K., et al. 2011. Mol Pharmacol. 79: 308-17. PMID: 20980443
3. EAPP: gatekeeper at the crossroad of apoptosis and p21-mediated cell-cycle arrest.  |  Andorfer, P. and Rotheneder, H. 2011. Oncogene. 30: 2679-90. PMID: 21258403
4. EAPP modulates the activity of p21 and Chk2.  |  Andorfer, P., et al. 2011. Cell Cycle. 10: 2077-82. PMID: 21572256
5. Regulation of the MDR1 promoter by E2F1 and EAPP.  |  Andorfer, P. and Rotheneder, H. 2013. FEBS Lett. 587: 1504-9. PMID: 23542036
6. Spatiotemporal Expression of EAPP Modulates Neuronal Apoptosis and Reactive Astrogliosis After Spinal Cord Injury.  |  Chen, M., et al. 2015. J Cell Biochem. 116: 1381-90. PMID: 25704466
7. Genetic expression analysis of E2F-associated phosphoprotein in stress responses in the mouse.  |  Duan, C., et al. 2016. Gene. 581: 130-8. PMID: 26802973
8. Syk-Targeted, a New 3-Arylbenzofuran Derivative EAPP-2 Blocks Airway Inflammation of Asthma-COPD Overlap in vivo and in vitro.  |  Li, S., et al. 2021. J Inflamm Res. 14: 2173-2185. PMID: 34079326
9. Endothelium-specific deletion of amyloid-β precursor protein exacerbates endothelial dysfunction induced by aging.  |  d'Uscio, LV. and Katusic, ZS. 2021. Aging (Albany NY). 13: 19165-19185. PMID: 34382945
10. Efficacy and safety of eliapixant in endometriosis-associated pelvic pain: the randomized, placebo-controlled phase 2b SCHUMANN study.  |  Parke, S., et al. 2024. BMC Womens Health. 24: 353. PMID: 38890641

The preferred EAPP antibody is EAPP Antibody (C-9): sc-365756.