EAAT2 Antibody (E-1): sc-365634

EAAT2 Antibody (E-1) is a mouse monoclonal IgG2b kappa light chain antibody that detects EAAT2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EAAT2 (E-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. EAAT2 protein, also known as solute carrier family 1 member 2 (SLC1A2) or glutamate transporter 1 (GLT-1), plays a crucial role in the central nervous system by mediating the uptake of the excitatory neurotransmitter L-glutamate, which is vital for maintaining synaptic homeostasis and preventing excitotoxicity. EAAT2 protein is predominantly localized in astrocytes and is essential for clearing excess glutamate from the synaptic cleft following neurotransmission, thereby protecting neurons from potential damage caused by glutamate accumulation. Proper functioning of EAAT2 is critical for normal brain function, as dysregulation of glutamate transport can lead to various neurological disorders, including epilepsy, neurodegenerative diseases, and mood disorders. EAAT2′s expression is primarily restricted to the brain, distinguishing EAAT2 from other glutamate transporters like EAAT1 and EAAT3, which are also found in non-nervous tissues. anti-EAAT2 antibody (E-1) allows researchers to explore the intricate mechanisms of glutamate transport and its implications in health and disease.

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EAAT2 Antibody (E-1) References:

1. Aberrant expression of the glutamate transporter excitatory amino acid transporter 1 (EAAT1) in Alzheimer's disease.  |  Scott, HL., et al. 2002. J Neurosci. 22: RC206. PMID: 11826152
2. Regulation of the glutamate transporter EAAT1 by the ubiquitin ligase Nedd4-2 and the serum and glucocorticoid-inducible kinase isoforms SGK1/3 and protein kinase B.  |  Boehmer, C., et al. 2003. J Neurochem. 86: 1181-8. PMID: 12911626
3. Transcriptional regulation of human excitatory amino acid transporter 1 (EAAT1): cloning of the EAAT1 promoter and characterization of its basal and inducible activity in human astrocytes.  |  Kim, SY., et al. 2003. J Neurochem. 87: 1485-98. PMID: 14713304
4. Midazolam contributes to neuroprotection against hypoxia/reoxygenation-induced brain injury in neonatal rats via regulation of EAAT2.  |  Tang, Z., et al. 2020. Brain Res Bull. 161: 136-146. PMID: 32433937
5. EAAT2 as a therapeutic research target in Alzheimer's disease: A systematic review.  |  Wood, OWG., et al. 2022. Front Neurosci. 16: 952096. PMID: 36033606
6. PS1/gamma-secretase acts as rogue chaperone of glutamate transporter EAAT2/GLT-1 in Alzheimer's disease.  |  Perrin, F., et al. 2024. Acta Neuropathol Commun. 12: 166. PMID: 39434170
7. Functional comparisons of three glutamate transporter subtypes cloned from human motor cortex.  |  Arriza, JL., et al. 1994. J Neurosci. 14: 5559-69. PMID: 7521911
8. Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2.  |  Kirschner, MA., et al. 1994. Genomics. 24: 218-24. PMID: 7698742
9. Glutamate transporter mRNA expression in proliferative zones of the developing and adult murine CNS.  |  Sutherland, ML., et al. 1996. J Neurosci. 16: 2191-207. PMID: 8601800
10. Human high affinity, Na(+)-dependent L-glutamate/L-aspartate transporter GLAST-1 (EAAT-1): gene structure and localization to chromosome 5p11-p12.  |  Stoffel, W., et al. 1996. FEBS Lett. 386: 189-93. PMID: 8647279
11. Nuclear disintegration as a leading step of glutamate excitotoxicity in brain neurons.  |  Ikeda, J., et al. 1996. J Neurosci Res. 43: 613-22. PMID: 8833096
12. High-affinity glutamate transporters in the rat retina: a major role of the glial glutamate transporter GLAST-1 in transmitter clearance.  |  Rauen, T., et al. 1998. Cell Tissue Res. 291: 19-31. PMID: 9394040

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EAAT2 Antibody (E-1) has 48 citations in a variety of scientific publications.

Primary antibodies like Anti-EAAT2 Antibody (E-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EAAT2 Antibody (E-1) Specifications

Alternate Product Name: SLC1A2

Product Clone Name: E-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-85

Epitope Target: EAAT2

Epitope Species: human

Product Reactivity/Detection: EAAT2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 48 citations in various scientific publications

Available Conjugates/Forms for EAAT2 Antibody (E-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin