EAAT1 Antibody (A-3): sc-515839

EAAT1 Antibody (A-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects EAAT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). EAAT1 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The excitatory amino acid transporter 1 (EAAT1), also known as SLC1A3, plays a crucial role in maintaining synaptic homeostasis by clearing excess extracellular glutamate, a neurotransmitter that, when accumulated, can lead to excitotoxicity and neuronal damage. EAAT1 is primarily located in glial cells and pre-synaptic glutamatergic nerve endings, where EAAT1 facilitates the reuptake of L-glutamate and D-aspartate, thereby terminating the postsynaptic action of these neurotransmitters. This function is vital for preventing overstimulation of neurons and ensuring proper neuronal signaling. Additionally, EAAT1 expression on the cell surface is regulated by insulin-like growth factor 1, which activates phosphatidylinositol-3-kinase, highlighting EAAT1′s importance in both neural and non-neural tissues. With EAAT1′s ability to protect neurons from excitotoxicity and involvement in neurotransmitter regulation, EAAT1 serves as a significant target for research in neurobiology and related fields.

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EAAT1 Antibody (A-3) References:

1. Aberrant expression of the glutamate transporter excitatory amino acid transporter 1 (EAAT1) in Alzheimer's disease.  |  Scott, HL., et al. 2002. J Neurosci. 22: RC206. PMID: 11826152
2. Regulation of the glutamate transporter EAAT1 by the ubiquitin ligase Nedd4-2 and the serum and glucocorticoid-inducible kinase isoforms SGK1/3 and protein kinase B.  |  Boehmer, C., et al. 2003. J Neurochem. 86: 1181-8. PMID: 12911626
3. Transcriptional regulation of human excitatory amino acid transporter 1 (EAAT1): cloning of the EAAT1 promoter and characterization of its basal and inducible activity in human astrocytes.  |  Kim, SY., et al. 2003. J Neurochem. 87: 1485-98. PMID: 14713304
4. Caveolin-1 Sensitivity of Excitatory Amino Acid Transporters EAAT1, EAAT2, EAAT3, and EAAT4.  |  Abousaab, A., et al. 2016. J Membr Biol. 249: 239-49. PMID: 26690923
5. Glial Glutamate Transporter-Mediated Plasticity: System xc-/xCT/SLC7A11 and EAAT1/2 in Brain Diseases.  |  Dahlmanns, M., et al. 2023. Front Biosci (Landmark Ed). 28: 57. PMID: 37005761
6. Excitatory amino acid transporter 1 supports adult hippocampal neural stem cell self-renewal.  |  Rieskamp, JD., et al. 2023. iScience. 26: 107068. PMID: 37534178
7. Functional comparisons of three glutamate transporter subtypes cloned from human motor cortex.  |  Arriza, JL., et al. 1994. J Neurosci. 14: 5559-69. PMID: 7521911
8. Mouse excitatory amino acid transporter EAAT2: isolation, characterization, and proximity to neuroexcitability loci on mouse chromosome 2.  |  Kirschner, MA., et al. 1994. Genomics. 24: 218-24. PMID: 7698742
9. Glutamate transporter mRNA expression in proliferative zones of the developing and adult murine CNS.  |  Sutherland, ML., et al. 1996. J Neurosci. 16: 2191-207. PMID: 8601800
10. Human high affinity, Na(+)-dependent L-glutamate/L-aspartate transporter GLAST-1 (EAAT-1): gene structure and localization to chromosome 5p11-p12.  |  Stoffel, W., et al. 1996. FEBS Lett. 386: 189-93. PMID: 8647279
11. Nuclear disintegration as a leading step of glutamate excitotoxicity in brain neurons.  |  Ikeda, J., et al. 1996. J Neurosci Res. 43: 613-22. PMID: 8833096
12. High-affinity glutamate transporters in the rat retina: a major role of the glial glutamate transporter GLAST-1 in transmitter clearance.  |  Rauen, T., et al. 1998. Cell Tissue Res. 291: 19-31. PMID: 9394040

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-EAAT1 Antibody (A-3) has 21 citations in a variety of scientific publications.

Primary antibodies like Anti-EAAT1 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

EAAT1 Antibody (A-3) Specifications

Alternate Product Name: SLC1A3

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-50

Epitope Target: EAAT1

Epitope Species: human

Product Reactivity/Detection: EAAT1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 21 citations in various scientific publications

Available Conjugates/Forms for EAAT1 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin