E2A Antibody (G-2): sc-133075
- E2A Antibody (G-2) is a mouse monoclonal IgG1 κ E2A antibody, cited in 8 publications, provided at 200 µg/ml
- raised against amino acids 1-649 of E2A of human origin
- E2A Antibody (G-2) is recommended for detection of E2A isoforms E12 and E47 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-E2A Antibody (G-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-133075 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for E2A Antibody (G-2) for WB and IHC(P) applications. These reagents are now offered in bundles with E2A Antibody (G-2) (see ordering information below).
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E2A Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects E2A protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-E2A antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E2A protein, also known as Transcription factor 3 (TCF-3), plays a crucial role in regulating gene expression during B cell maturation and is part of the helix-loop-helix (HLH) protein family, which is essential for muscle cell differentiation. E2A interacts with other HLH proteins, such as MyoD and myogenin, to form heterodimers that bind to E box sequences in the regulatory regions of muscle-specific genes, thereby influencing lineage-specific gene expression. E2A′s ability to form both homo- and hetero-oligomers allows for distinguishing between closely related E box proteins, highlighting E2A′s importance in the precise regulation of gene expression during development. This multifaceted role underscores E2A′s significance in both hematopoietic and muscle cell differentiation, making E2A (G-2) monoclonal antibody an invaluable tool for researchers studying these critical biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
E2A Antibody (G-2) References:
- Regulation of early lymphocyte development by E2A family proteins. | Greenbaum, S. and Zhuang, Y. 2002. Semin Immunol. 14: 405-14. PMID: 12457613
- E2A proteins: regulators of cell phenotype in normal physiology and disease. | Slattery, C., et al. 2008. Int J Biochem Cell Biol. 40: 1431-6. PMID: 17604208
- Differential involvement of E2A-corepressor interactions in distinct leukemogenic pathways. | Gow, CH., et al. 2014. Nucleic Acids Res. 42: 137-52. PMID: 24064250
- Repression of TCF3/E2A contributes to Hodgkin lymphomagenesis. | Guan, H., et al. 2016. Oncotarget. 7: 36854-36864. PMID: 27166193
- E2A attenuates tumor-initiating capacity of colorectal cancer cells via the Wnt/beta-catenin pathway. | Zhao, H., et al. 2019. J Exp Clin Cancer Res. 38: 276. PMID: 31234887
- The transcription factor E2A drives neural differentiation in pluripotent cells. | Rao, C., et al. 2020. Development. 147: PMID: 32487737
- Mediator subunit MED1 is required for E2A-PBX1-mediated oncogenic transcription and leukemic cell growth. | Lee, YL., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33542097
- E2A-regulated epigenetic landscape promotes memory CD8 T cell differentiation. | Schauder, DM., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33859041
- The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells. | Zhang, M., et al. 2022. J Cell Mol Med. 26: 1128-1143. PMID: 35001521
- The transcription factor E2A can bind to and cleave single-stranded immunoglobulin heavy chain locus DNA. | Grundström, C. and Grundström, T. 2023. Mol Immunol. 153: 51-59. PMID: 36434987
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
E2A Antibody (G-2) | sc-133075 | 200 µg/ml | $322.00 | |||
E2A Antibody (G-2): m-IgG Fc BP-HRP Bundle | sc-528740 | 200 µg Ab; 10 µg BP | $361.00 | |||
E2A Antibody (G-2): m-IgGκ BP-HRP Bundle | sc-521223 | 200 µg Ab, 40 µg BP | $361.00 | |||
E2A Antibody (G-2): m-IgG1 BP-HRP Bundle | sc-543106 | 200 µg Ab; 20 µg BP | $361.00 | |||
E2A Antibody (G-2) X | sc-133075 X | 200 µg/0.1 ml | $322.00 | |||
E2A Antibody (G-2) AC | sc-133075 AC | 500 µg/ml, 25% agarose | $424.00 | |||
E2A Antibody (G-2) HRP | sc-133075 HRP | 200 µg/ml | $322.00 | |||
E2A Antibody (G-2) FITC | sc-133075 FITC | 200 µg/ml | $336.00 | |||
E2A Antibody (G-2) PE | sc-133075 PE | 200 µg/ml | $349.00 | |||
E2A Antibody (G-2) Alexa Fluor® 488 | sc-133075 AF488 | 200 µg/ml | $364.00 | |||
E2A Antibody (G-2) Alexa Fluor® 546 | sc-133075 AF546 | 200 µg/ml | $364.00 | |||
E2A Antibody (G-2) Alexa Fluor® 594 | sc-133075 AF594 | 200 µg/ml | $364.00 | |||
E2A Antibody (G-2) Alexa Fluor® 647 | sc-133075 AF647 | 200 µg/ml | $364.00 | |||
E2A Antibody (G-2) Alexa Fluor® 680 | sc-133075 AF680 | 200 µg/ml | $364.00 | |||
E2A Antibody (G-2) Alexa Fluor® 790 | sc-133075 AF790 | 200 µg/ml | $364.00 |