E2A Antibody (Yae): sc-416

E2A Antibody (Yae) is a mouse monoclonal IgG1 kappa light chain antibody that detects E2A protein, also known as ITF-1, in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. E2A monoclonal antibody (Yae) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. E2A protein, a member of the helix-loop-helix transcription factor family, plays a crucial role in regulating gene expression during B cell maturation and myogenic differentiation. E2A protein is essential for the formation of heterodimers with Myo D and other basic helix-loop-helix proteins, which bind to E box elements in the regulatory regions of muscle-specific genes. This interaction is vital for proper differentiation of muscle cells, as E2A protein ensures the activation of genes necessary for muscle development. E2A protein forms both homo- and hetero-oligomers allowing for precise regulation of gene expression, making E2A protein a key player in lineage-specific transcriptional control. E2A monoclonal antibody (Yae) enables researchers to gain insights into mechanisms of muscle cell differentiation and broader implications of transcription factor interactions in various biological processes.

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E2A Antibody (Yae) References:

1. HEB, a helix-loop-helix protein related to E2A and ITF2 that can modulate the DNA-binding ability of myogenic regulatory factors.  |  Hu, JS., et al. 1992. Mol Cell Biol. 12: 1031-42. PMID: 1312219
2. Molecular characterization of helix-loop-helix peptides.  |  Anthony-Cahill, SJ., et al. 1992. Science. 255: 979-83. PMID: 1312255
3. Accelerated Notch-dependent degradation of E47 proteins in aged B cell precursors is associated with increased ERK MAPK activation.  |  King, AM., et al. 2007. J Immunol. 178: 3521-9. PMID: 17339447
4. Herculin, a fourth member of the MyoD family of myogenic regulatory genes.  |  Miner, JH. and Wold, B. 1990. Proc Natl Acad Sci U S A. 87: 1089-93. PMID: 2300571
5. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence.  |  Murre, C., et al. 1989. Cell. 58: 537-44. PMID: 2503252
6. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.  |  Wright, WE., et al. 1989. Cell. 56: 607-17. PMID: 2537150
7. Identification of MRF4: a new member of the muscle regulatory factor gene family.  |  Rhodes, SJ. and Konieczny, SF. 1989. Genes Dev. 3: 2050-61. PMID: 2560751
8. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.  |  Braun, T., et al. 1989. EMBO J. 8: 701-9. PMID: 2721498
9. Transcription factors TEAD2 and E2A globally repress acetyl-CoA synthesis to promote tumorigenesis.  |  Park, S., et al. 2022. Mol Cell. 82: 4246-4261.e11. PMID: 36400009
10. The advances of E2A-PBX1 fusion in B-cell acute lymphoblastic Leukaemia.  |  Yang, M., et al. 2024. Ann Hematol. 103: 3385-3398. PMID: 38148344
11. The Function of E2A in B-Cell Development.  |  Miyazaki, M. and Miyazaki, K. 2024. Adv Exp Med Biol. 1459: 97-113. PMID: 39017841
12. Cell-specific expression of helix-loop-helix transcription factors encoded by the E2A gene.  |  Aronheim, A., et al. 1993. Nucleic Acids Res. 21: 1601-6. PMID: 8479911

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-E2A Antibody (Yae) has 66 citations in a variety of scientific publications.

Primary antibodies like Anti-E2A Antibody (Yae) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

E2A Antibody (Yae) Specifications

Alternate Product Name: TCF3

Product Clone Name: Yae

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 195-208

Epitope Target: E2A

Epitope Species: human

Product Reactivity/Detection: ITF-1, and E2A isoforms E12 and E47

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 66 citations in various scientific publications

Available Conjugates/Forms for E2A Antibody (Yae): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent