E2A Antibody (G-2): sc-133075

E2A Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects E2A protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-E2A antibody (G-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E2A protein, also known as Transcription factor 3 (TCF-3), plays a crucial role in regulating gene expression during B cell maturation and is part of the helix-loop-helix (HLH) protein family, which is essential for muscle cell differentiation. E2A interacts with other HLH proteins, such as MyoD and myogenin, to form heterodimers that bind to E box sequences in the regulatory regions of muscle-specific genes, thereby influencing lineage-specific gene expression. E2A′s ability to form both homo- and hetero-oligomers allows for distinguishing between closely related E box proteins, highlighting E2A′s importance in the precise regulation of gene expression during development. This multifaceted role underscores E2A′s significance in both hematopoietic and muscle cell differentiation, making E2A (G-2) monoclonal antibody an invaluable tool for researchers studying these critical biological processes.

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E2A Antibody (G-2) References:

1. Regulation of early lymphocyte development by E2A family proteins.  |  Greenbaum, S. and Zhuang, Y. 2002. Semin Immunol. 14: 405-14. PMID: 12457613
2. E2A proteins: regulators of cell phenotype in normal physiology and disease.  |  Slattery, C., et al. 2008. Int J Biochem Cell Biol. 40: 1431-6. PMID: 17604208
3. Differential involvement of E2A-corepressor interactions in distinct leukemogenic pathways.  |  Gow, CH., et al. 2014. Nucleic Acids Res. 42: 137-52. PMID: 24064250
4. Repression of TCF3/E2A contributes to Hodgkin lymphomagenesis.  |  Guan, H., et al. 2016. Oncotarget. 7: 36854-36864. PMID: 27166193
5. E2A attenuates tumor-initiating capacity of colorectal cancer cells via the Wnt/beta-catenin pathway.  |  Zhao, H., et al. 2019. J Exp Clin Cancer Res. 38: 276. PMID: 31234887
6. The transcription factor E2A drives neural differentiation in pluripotent cells.  |  Rao, C., et al. 2020. Development. 147: PMID: 32487737
7. Mediator subunit MED1 is required for E2A-PBX1-mediated oncogenic transcription and leukemic cell growth.  |  Lee, YL., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33542097
8. E2A-regulated epigenetic landscape promotes memory CD8 T cell differentiation.  |  Schauder, DM., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33859041
9. The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells.  |  Zhang, M., et al. 2022. J Cell Mol Med. 26: 1128-1143. PMID: 35001521
10. The transcription factor E2A can bind to and cleave single-stranded immunoglobulin heavy chain locus DNA.  |  Grundström, C. and Grundström, T. 2023. Mol Immunol. 153: 51-59. PMID: 36434987

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-E2A Antibody (G-2) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-E2A Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

E2A Antibody (G-2) Specifications

Alternate Product Name: TCF3

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-649

Epitope Target: E2A

Epitope Species: human

Product Reactivity/Detection: E2A isoforms E12 and E47

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for E2A Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent

The preferred E2A antibody is E2A Antibody (Yae): sc-416.