dystrophin Antibody (H-5): sc-365954
- dystrophin Antibody (H-5) is a mouse monoclonal IgG2b κ, cited in 6 publications, provided at 200 µg/ml
- raised against amino acids 801-1100 mapping within an internal region of dystrophin of human origin
- Anti-dystrophin Antibody (H-5) is recommended for detection of dystrophin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-dystrophin Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for dystrophin Antibody (H-5) for WB and IHC(P) applications. These reagents are now offered in bundles with dystrophin Antibody (H-5) (see ordering information below).
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Dystrophin Antibody (H-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects dystrophin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-dystrophin antibody (H-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dystrophin plays a crucial role in the dystrophin-glycoprotein complex (DGC), which serves as a vital link between the actin cytoskeleton within muscle fibers and the extracellular matrix, thereby maintaining muscle integrity and function. The absence or mutation of dystrophin is a primary factor in the development of Duchenne (DMD) and Becker (BMD) muscular dystrophies, highlighting its importance in muscle health. The human dystrophin gene spans 2.4 megabases with over 80 exons, producing a 14 kb mRNA that is regulated by at least eight independent tissue-specific promoters and two polyadenylation sites. Additionally, dystrophin mRNA can undergo differential splicing, resulting in a variety of transcripts that encode different protein isoforms. Dystrophin constitutes approximately 0.002% of total striated muscle protein and is localized at triadic junctions in skeletal muscle, where dystrophin plays a significant role in calcium ion homeostasis and the transmission of force during muscle contraction.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
dystrophin Antibody (H-5) References:
- Dystrophin: from non-ischemic cardiomyopathy to ischemic cardiomyopathy. | Zheng, QS., et al. 2008. Med Hypotheses. 71: 434-8. PMID: 18562127
- Myogenic regulation of dystrophin gene expression. | Klamut, HJ., et al. 1989. Br Med Bull. 45: 681-702. PMID: 2688823
- Necroptosis mediates myofibre death in dystrophin-deficient mice. | Morgan, JE., et al. 2018. Nat Commun. 9: 3655. PMID: 30194302
- Dystrophin deficiency promotes leukocyte recruitment in mdx mice. | Kranig, SA., et al. 2019. Pediatr Res. 86: 188-194. PMID: 31091530
- Effects of Mini-Dystrophin on Dystrophin-Deficient, Human Skeletal Muscle-Derived Cells. | Meng, J., et al. 2020. Int J Mol Sci. 21: PMID: 32998454
- Very Low Residual Dystrophin Quantity Is Associated with Milder Dystrophinopathy. | de Feraudy, Y., et al. 2021. Ann Neurol. 89: 280-292. PMID: 33159473
- Dystrophin Gene-Editing Stability Is Dependent on Dystrophin Levels in Skeletal but Not Cardiac Muscles. | Bengtsson, NE., et al. 2021. Mol Ther. 29: 1070-1085. PMID: 33160075
- Dystrophin and mini-dystrophin quantification by mass spectrometry in skeletal muscle for gene therapy development in Duchenne muscular dystrophy. | Farrokhi, V., et al. 2022. Gene Ther. 29: 608-615. PMID: 34737451
- Structure and assembly of the dystrophin glycoprotein complex. | Wan, L., et al. 2024. Nature.. PMID: 39663450
- Heterogeneity of dystrophin-associated proteins. | Yamamoto, H., et al. 1993. J Biochem. 114: 132-9. PMID: 8407865
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
dystrophin Antibody (H-5) | sc-365954 | 200 µg/ml | $316.00 | |||
dystrophin Antibody (H-5): m-IgG Fc BP-HRP Bundle | sc-529525 | 200 µg Ab; 10 µg BP | $354.00 | |||
dystrophin Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-522440 | 200 µg Ab, 40 µg BP | $354.00 | |||
dystrophin Antibody (H-5): m-IgG2b BP-HRP Bundle | sc-550122 | 200 µg Ab; 10 µg BP | $354.00 | |||
dystrophin Antibody (H-5) AC | sc-365954 AC | 500 µg/ml, 25% agarose | $416.00 | |||
dystrophin Antibody (H-5) HRP | sc-365954 HRP | 200 µg/ml | $316.00 | |||
dystrophin Antibody (H-5) FITC | sc-365954 FITC | 200 µg/ml | $330.00 | |||
dystrophin Antibody (H-5) PE | sc-365954 PE | 200 µg/ml | $343.00 | |||
dystrophin Antibody (H-5) Alexa Fluor® 488 | sc-365954 AF488 | 200 µg/ml | $357.00 | |||
dystrophin Antibody (H-5) Alexa Fluor® 546 | sc-365954 AF546 | 200 µg/ml | $357.00 | |||
dystrophin Antibody (H-5) Alexa Fluor® 594 | sc-365954 AF594 | 200 µg/ml | $357.00 | |||
dystrophin Antibody (H-5) Alexa Fluor® 647 | sc-365954 AF647 | 200 µg/ml | $357.00 | |||
dystrophin Antibody (H-5) Alexa Fluor® 680 | sc-365954 AF680 | 200 µg/ml | $357.00 | |||
dystrophin Antibody (H-5) Alexa Fluor® 790 | sc-365954 AF790 | 200 µg/ml | $357.00 |