Dynamin I/II Antibody (E-4): sc-390160

Dynamin I/II Antibody (E-4) is a mouse monoclonal IgG1 antibody that detects Dynamin I and Dynamin II in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Dynamin I and II are critical members of the dynamin family, functioning as GTPase and microtubule-associated proteins that play essential roles in endocytosis, synaptic transmission, and neurogenesis. Dynamin I is predominantly localized in the central nervous system, while Dynamin II is widely distributed throughout various tissues, with the highest expression levels found in the testis. The structure of Dynamin I and II includes SH3 and proline-rich domains, which are vital for mediating interactions with various effectors that regulate GTPase activity. These interactions facilitate the recruitment of Dynamin I to clathrin-coated pits through SH3 domain interactions with amphiphysin, a protein highly expressed in the brain. Understanding the structural features and functional interactions of Dynamin I and II is important for elucidating their roles in cellular processes and potential implications in neurological disorders. Anti-Dynamin I/II antibody (E-4) is an invaluable tool for researchers investigating these dynamic proteins and their contributions to cellular mechanisms.

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Dynamin I/II Antibody (E-4) References:

1. Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin.  |  Cousin, MA., et al. 2001. J Neurochem. 76: 105-16. PMID: 11145983
2. Cophosphorylation of amphiphysin I and dynamin I by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles.  |  Tomizawa, K., et al. 2003. J Cell Biol. 163: 813-24. PMID: 14623869
3. Dynamin I phosphorylation and the control of synaptic vesicle endocytosis.  |  Smillie, KJ. and Cousin, MA. 2005. Biochem Soc Symp. 87-97. PMID: 15649133
4. Complexes of syndapin II with dynamin II promote vesicle formation at the trans-Golgi network.  |  Kessels, MM., et al. 2006. J Cell Sci. 119: 1504-16. PMID: 16551695
5. Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis.  |  Anggono, V., et al. 2006. Nat Neurosci. 9: 752-60. PMID: 16648848
6. Syndapin I and endophilin I bind overlapping proline-rich regions of dynamin I: role in synaptic vesicle endocytosis.  |  Anggono, V. and Robinson, PJ. 2007. J Neurochem. 102: 931-43. PMID: 17437541
7. Myristyl trimethyl ammonium bromide and octadecyl trimethyl ammonium bromide are surface-active small molecule dynamin inhibitors that block endocytosis mediated by dynamin I or dynamin II.  |  Quan, A., et al. 2007. Mol Pharmacol. 72: 1425-39. PMID: 17702890
8. The pthaladyns: GTP competitive inhibitors of dynamin I and II GTPase derived from virtual screening.  |  Odell, LR., et al. 2010. J Med Chem. 53: 5267-80. PMID: 20575553
9. Phosphorylation of dynamin I and synaptic-vesicle recycling.  |  Robinson, PJ., et al. 1994. Trends Neurosci. 17: 348-53. PMID: 7526507
10. Ubiquitously expressed dynamin-II has a higher intrinsic GTPase activity and a greater propensity for self-assembly than neuronal dynamin-I.  |  Warnock, DE., et al. 1997. Mol Biol Cell. 8: 2553-62. PMID: 9398675

The preferred Dynamin I antibody is Dynamin I Antibody (D5): sc-12724.