Date published: 2025-11-4
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Dynamin II Antibody (G-4) - Western Blotting - Image 398988
Dynamin II 抗体 (G-4): sc-166669. HeLa (A), K-562 (B), IMR-32 (C), NTERA-2 cl. D1 (D), SK-N-SH (E) 和 NIH/3T3 (F) 全细胞裂解液中 Dynamin II 表达的 Western 印迹分析. 所用检测试剂: m-IgG3 BP-HRP: sc-533670.
Dynamin II 抗体 (G-4): sc-166669. HeLa (A), K-562 (B), IMR-32 (C), NTERA-2 cl. D1 (D), SK-N-SH (E) 和 NIH/3T3 (F) 全细胞裂解液中 Dynamin II 表达的 Western 印迹分析. 所用检测试剂: m-IgG3 BP-HRP: sc-533670.

Dynamin II 抗体 (G-4): sc-166669

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寫評論提問

说明书
  • Dynamin II 抗体 G-4 是小鼠单克隆 IgG3 κ，Dynamin II抗体, 在16篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Dynamin II的C-terminus的氨基酸853-870之间
  • Dynamin II 抗体 (G-4) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Dynamin II; 也和以下物种反应，包括： 和 equine, bovine, porcine and canine; 可能与 Dynamin III产生交联反应
  • 抗Dynamin II抗体（G-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG3 BP-HRP和m-IgGκ BP-HRP是Dynamin II Antibody (G-4) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与Dynamin II Antibody (G-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Dynamin抗体，包括Dynamin I, Dynamin II和Dynamin I/II

Dynamin II抗体（G-4）是一种IgG3 κ小鼠单克隆Dynamin II抗体（也称为Dynamin II抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的Dynamin II蛋白。Dynamin II抗体（G-4）既可以是非偶联的抗Dynamin II抗体形式，也可以是多种偶联形式的抗Dynamin II抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Dynamin家族的成员，包括Dynamin I和Dynamin II，是GPTase、微管相关蛋白，它们参与内吞作用、突触传递和神经发生。Dynamin I定位于中枢神经系统，而Dynamin II则普遍分布，其中在睾丸中的表达量最高。两种dynamin蛋白都含有SH3和富含脯氨酸的结构域，这些结构域介导dynamins与其GTPase活性的效应器之间的相互作用。与这些效应物（包括微管、酸性磷脂和含有SH3结构域的蛋白质）的相互作用是快速内吞所必需的。 Dynamin I似乎通过SH3结构域与amphiphysin（一种在脑中高表达的蛋白质）的相互作用被募集到网格蛋白包被的凹坑中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dynamin II 抗体 (G-4) 参考文献:

  1. 辛达平Ⅱ与达能素Ⅱ的复合物促进了跨高尔基网络囊泡的形成  |  Kessels, MM., et al. 2006. J Cell Sci. 119: 1504-16. PMID: 16551695
  2. 肉豆蔻基三甲基溴化铵和十八烷基三甲基溴化铵是表面活性的小分子动态素抑制剂，可阻断动态素 I 或动态素 II 介导的内吞作用。  |  Quan, A., et al. 2007. Mol Pharmacol. 72: 1425-39. PMID: 17702890
  3. 生长因子诱导的达纳明与信号转导蛋白的结合涉及到对不同的富含脯氨酸的达纳明序列的分选。  |  Scaife, R., et al. 1994. EMBO J. 13: 2574-82. PMID: 8013457
  4. 鉴定大鼠组织中普遍表达的同工酶达纳明 2。  |  Cook, TA., et al. 1994. Proc Natl Acad Sci U S A. 91: 644-8. PMID: 8290576
  5. 多种动态蛋白的差异表达和调控。  |  Sontag, JM., et al. 1994. J Biol Chem. 269: 4547-54. PMID: 8308025
  6. SH3结构域的结合位点可将动态蛋白靶向涂布坑。  |  Shpetner, HS., et al. 1996. J Biol Chem. 271: 13-6. PMID: 8550547
  7. 达纳明富含脯氨酸的基本区域在 Src 同源 3 结构域结合和内吞过程中的作用  |  Okamoto, PM., et al. 1997. J Biol Chem. 272: 11629-35. PMID: 9111080
  8. 虹彩蛋白的 SH3 结构域与达能蛋白的富脯氨酸结构域结合在一个单一位点上，该位点定义了一个新的 SH3 结合共识序列。  |  Grabs, D., et al. 1997. J Biol Chem. 272: 13419-25. PMID: 9148966
  9. 面包酵母、小麦胚芽和龙虾尾肌肉中m-钙蛋白酶、钙蛋白酶、一氧化氮合酶、髓鞘碱性蛋白和达因明Ⅱ的免疫活性。  |  Kuo, WN., et al. 1996. Cytobios. 87: 251-63. PMID: 9214726
  10. 虹彩蛋白 SH3 结构域对受体介导的内吞作用的抑制。  |  Wigge, P., et al. 1997. Curr Biol. 7: 554-60. PMID: 9259551
  11. PH 结构域和 SH3 结合结构域在达能素功能中的作用  |  Scaife, RM. and Margolis, RL. 1997. Cell Signal. 9: 395-401. PMID: 9376220
  12. 非泛素表达的动态素-II比神经元动态素-I具有更高的内在GTP酶活性和更强的自组装倾向。  |  Warnock, DE., et al. 1997. Mol Biol Cell. 8: 2553-62. PMID: 9398675

订购信息

产品名称产品编号规格价格数量收藏夹

Dynamin II 抗体 (G-4)

sc-166669
200 µg/ml
$316.00

Dynamin II (G-4): m-IgGκ BP-HRP 套装

sc-521703
200 µg Ab, 40 µg BP
$354.00

Dynamin II (G-4): m-IgG3 BP-HRP 套装

sc-550337
200 µg Ab; 40 µg BP
$354.00

Dynamin II 抗体 (G-4) AC

sc-166669 AC
500 µg/ml, 25% agarose
$416.00

Dynamin II 抗体 (G-4) HRP

sc-166669 HRP
200 µg/ml
$316.00

Dynamin II 抗体 (G-4) FITC

sc-166669 FITC
200 µg/ml
$330.00

Dynamin II 抗体 (G-4) PE

sc-166669 PE
200 µg/ml
$343.00

Dynamin II 抗体 (G-4) Alexa Fluor® 488

sc-166669 AF488
200 µg/ml
$357.00

Dynamin II 抗体 (G-4) Alexa Fluor® 546

sc-166669 AF546
200 µg/ml
$357.00

Dynamin II 抗体 (G-4) Alexa Fluor® 594

sc-166669 AF594
200 µg/ml
$357.00

Dynamin II 抗体 (G-4) Alexa Fluor® 647

sc-166669 AF647
200 µg/ml
$357.00

Dynamin II 抗体 (G-4) Alexa Fluor® 680

sc-166669 AF680
200 µg/ml
$357.00

Dynamin II 抗体 (G-4) Alexa Fluor® 790

sc-166669 AF790
200 µg/ml
$357.00

Dynamin II (G-4) 中和勝肽

sc-166669 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-166669 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from perfectI feel it was fate that I came across these wonderful antibodies.　
Date published: 2023-12-10
Rated 1 out of 5 by from Not working!We tested this antibody on cardiac and skeletal tissue samples using a 1:100 dilution with 5% milk or 2% BSA. Unfortunately, we did not observe any bands. I'm unsure about the next steps. Could it be a problem with the batch?
Date published: 2023-11-16
Rated 5 out of 5 by from Great AntibodyI bought this antibody two weeks ago and used it in WB analysis. This DNM 2 antibody could specifically recognize the protein of Dynamin II at the expected MW size (100 kD). In my experiment, I use the antibody to analyze Dynamin II protein expression in HEL cell extracts. I incubated the PVDF membrane with this antibody in a dilution of 1:500 for 2 hours. It showed a bright signal. This is a very good antibody!
Date published: 2020-09-11
Rated 5 out of 5 by from clear band in TM4I diluted this antibody in 1:500, and incubated with TM4‘s WB sample. The band was clear and single.
Date published: 2018-05-09
Rated 5 out of 5 by from Great FeaturesI tested this antibody a couple of weeks ago and I found it excellent!
Date published: 2018-03-02
Rated 5 out of 5 by from Antibodies nicely working for WBImage shows analysis of Dynamin II level in SKOV3 cells upon knock-down of DNM2 gene using CRISPR/Cas9. Dynamin II Antibodies (G-4): sc-166669 detect a band of approximately 100 kDa (corresponding to molecular weight of DNM2) and are specific.
Date published: 2018-02-23
Rated 4 out of 5 by from excellent antibody for immunofluorescenceI used it for immunostaining HeLa cells (1:50 and 1:100 dilution) and works well for intracellular puncta.
Date published: 2017-11-05
Rated 5 out of 5 by from wonderfulThe dynamin 2 antibody we used is a sample from Santa, we tested it in MDCK cells by IF. It works really good!
Date published: 2017-10-07
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Dynamin II抗体 (G-4) is rated 4.1 out of 5 by 10.
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