Dynamin II Antibody (G-4): sc-166669

Dynamin II Antibody (G-4) is a mouse monoclonal IgG3 kappa light chain antibody that detects Dynamin II protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Dynamin II antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dynamin II plays a crucial role in endocytosis, a process essential for cellular uptake of nutrients and signaling molecules, as well as synaptic transmission and neurogenesis. Unlike Dynamin I, which is primarily localized to the central nervous system, Dynamin II is ubiquitously expressed, with the highest levels found in the testis, indicating its importance in reproductive biology. The structure of Dynamin II includes SH3 and proline-rich domains that facilitate interactions with various effectors, such as microtubules and SH3 domain-containing proteins, which are vital for its GTPase activity and the rapid endocytosis process. These interactions are critical for maintaining cellular homeostasis and facilitating communication between cells, underscoring the significance of Dynamin II in both physiological and pathological contexts.

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Dynamin II Antibody (G-4) References:

1. Complexes of syndapin II with dynamin II promote vesicle formation at the trans-Golgi network.  |  Kessels, MM., et al. 2006. J Cell Sci. 119: 1504-16. PMID: 16551695
2. Myristyl trimethyl ammonium bromide and octadecyl trimethyl ammonium bromide are surface-active small molecule dynamin inhibitors that block endocytosis mediated by dynamin I or dynamin II.  |  Quan, A., et al. 2007. Mol Pharmacol. 72: 1425-39. PMID: 17702890
3. Growth factor-induced binding of dynamin to signal transduction proteins involves sorting to distinct and separate proline-rich dynamin sequences.  |  Scaife, R., et al. 1994. EMBO J. 13: 2574-82. PMID: 8013457
4. Identification of dynamin 2, an isoform ubiquitously expressed in rat tissues.  |  Cook, TA., et al. 1994. Proc Natl Acad Sci U S A. 91: 644-8. PMID: 8290576
5. Differential expression and regulation of multiple dynamins.  |  Sontag, JM., et al. 1994. J Biol Chem. 269: 4547-54. PMID: 8308025
6. A binding site for SH3 domains targets dynamin to coated pits.  |  Shpetner, HS., et al. 1996. J Biol Chem. 271: 13-6. PMID: 8550547
7. Role of the basic, proline-rich region of dynamin in Src homology 3 domain binding and endocytosis.  |  Okamoto, PM., et al. 1997. J Biol Chem. 272: 11629-35. PMID: 9111080
8. The SH3 domain of amphiphysin binds the proline-rich domain of dynamin at a single site that defines a new SH3 binding consensus sequence.  |  Grabs, D., et al. 1997. J Biol Chem. 272: 13419-25. PMID: 9148966
9. Immunoreactivities of m-calpain, calpastatin, nitric oxide synthase, myelin basic protein and dynamin II in baker's yeast, wheat germ and lobster tail muscle.  |  Kuo, WN., et al. 1996. Cytobios. 87: 251-63. PMID: 9214726
10. Inhibition of receptor-mediated endocytosis by the amphiphysin SH3 domain.  |  Wigge, P., et al. 1997. Curr Biol. 7: 554-60. PMID: 9259551
11. The role of the PH domain and SH3 binding domains in dynamin function.  |  Scaife, RM. and Margolis, RL. 1997. Cell Signal. 9: 395-401. PMID: 9376220
12. Ubiquitously expressed dynamin-II has a higher intrinsic GTPase activity and a greater propensity for self-assembly than neuronal dynamin-I.  |  Warnock, DE., et al. 1997. Mol Biol Cell. 8: 2553-62. PMID: 9398675

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dynamin II Antibody (G-4) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-Dynamin II Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dynamin II Antibody (G-4) Specifications

Alternate Product Name: Dynamin II

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 853-870

Epitope Target: Dynamin II

Epitope Species: human

Product Reactivity/Detection: Dynamin II

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine, porcine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for Dynamin II Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin