Dynactin 1 Antibody (A-2): sc-365274

Dynactin 1 Antibody (A-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Dynactin 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Dynactin 1 antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dynactin 1 plays a crucial role in cellular transport processes as a component of the dynactin complex, which is essential for proper functioning of the Dynein motor protein. This complex facilitates movement of organelles along microtubules, ensuring effective distribution of cellular components. The interaction between Dynactin and Dynein is vital for various cellular activities, including organelle transport, mitotic spindle formation, and cytokinesis, maintaining cellular organization and function. Dynactin 1 is enriched in neurons, contributing to transport of synaptic vesicles and other critical cargo, underscoring significance in neuronal health and signaling. The Dynactin complex comprises several subunits, including p22, p50, p62, p150 (also known as Glued), and ARP-1, with p135 splice variant being neuron-specific and lacking microtubule binding ability, illustrating complexity and specificity of Dynactin′s role in cellular dynamics.

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Dynactin 1 Antibody (A-2) References:

1. Cytoplasmic dynein and dynactin in cell division and intracellular transport.  |  Karki, S. and Holzbaur, EL. 1999. Curr Opin Cell Biol. 11: 45-53. PMID: 10047518
2. The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain.  |  Berrueta, L., et al. 1999. Curr Biol. 9: 425-8. PMID: 10226031
3. M phase phosphorylation of cytoplasmic dynein intermediate chain and p150(Glued).  |  Huang, CY., et al. 1999. J Biol Chem. 274: 14262-9. PMID: 10318847
4. Cytoplasmic dynein and dynactin as likely candidates for microtubule-dependent apical targeting of pancreatic zymogen granules.  |  Kraemer, J., et al. 1999. Eur J Cell Biol. 78: 265-77. PMID: 10350215
5. Integrin-linked kinase links dynactin-1/dynactin-2 with cortical integrin receptors to orient the mitotic spindle relative to the substratum.  |  Morris, EJ., et al. 2015. Sci Rep. 5: 8389. PMID: 25669897
6. DCTN1-ALK gene fusion in inflammatory myofibroblastic tumor (IMT) of the CNS.  |  Vidrine, DW., et al. 2021. Childs Nerv Syst. 37: 2147-2151. PMID: 34014367
7. Functional analysis of dynactin and cytoplasmic dynein in slow axonal transport.  |  Dillman, JF., et al. 1996. J Neurosci. 16: 6742-52. PMID: 8824315
8. Functionally distinct isoforms of dynactin are expressed in human neurons.  |  Tokito, MK., et al. 1996. Mol Biol Cell. 7: 1167-80. PMID: 8856662
9. The interaction between cytoplasmic dynein and dynactin is required for fast axonal transport.  |  Waterman-Storer, CM., et al. 1997. Proc Natl Acad Sci U S A. 94: 12180-5. PMID: 9342383
10. The role of the dynactin complex in intracellular motility.  |  Holleran, EA., et al. 1998. Int Rev Cytol. 182: 69-109. PMID: 9522459
11. Characterization of the p22 subunit of dynactin reveals the localization of cytoplasmic dynein and dynactin to the midbody of dividing cells.  |  Karki, S., et al. 1998. J Cell Biol. 142: 1023-34. PMID: 9722614
12. Human DCTN1: genomic structure and evaluation as a candidate for Alström syndrome.  |  Collin, GB., et al. 1998. Genomics. 53: 359-64. PMID: 9799602

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dynactin 1 Antibody (A-2) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Dynactin 1 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dynactin 1 Antibody (A-2) Specifications

Alternate Product Name: Dynactin 1

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 964-1263

Epitope Target: Dynactin 1

Epitope Species: human

Product Reactivity/Detection: p150 and p135 splice variants of Dynactin 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Dynactin 1 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin