dsg3 Antibody (5G11) recommended for detection of dsg3 of mouse, rat and human origin by WB, IP, IF and IHC(P)

dsg3 Antibody (5G11): sc-53487

dsg3 Antibody (5G11) is rated 4.0 out of 5 by 2.
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Datasheets

    • dsg3 Antibody (5G11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against A-431 cell shavings
    • Anti-dsg3 Antibody (5G11) is recommended for detection of dsg3 of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with dsg1 or dsg2
    • Anti-dsg3 Antibody (5G11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of dsg3 (5G11): sc-53487.
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What blocking solution should I use with dsg3 (5G11): sc-53487 monoclonal antibody for Western blot?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
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Rated 5 out of 5 by from Good antibody This is a nice and specific dsg3 antibody clone, but not as strong as our the alternate dsg3 Antibody (5H10), sc-23912.
Date published: 2017-02-01
Rated 3 out of 5 by from Robust bands seen in WB using SCC Robust bands seen in WB using SCC-25 whole cell lysate. -SCBT QC
Date published: 2013-12-09
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