dsg3 Antibody (5H10): sc-23912
- dsg3 Antibody (5H10) is a mouse monoclonal IgG1 κ dsg3 antibody, cited in 19 publications, provided at 200 µg/ml
- raised against an extracellular domain of human dsg3
- dsg3 Antibody (5H10) is recommended for detection of desmoglein 3 of human origin by WB, IP and IF; non cross-reactive with dsg1, dsg2, or with mouse proteins
- Anti-dsg3 Antibody (5H10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for dsg3 Antibody (5H10) for WB applications. These reagents are now offered in bundles with dsg3 Antibody (5H10) (see ordering information below).
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dsg3 Antibody (5H10) is a mouse monoclonal IgG1 kappa light chain antibody that detects dsg3 protein of human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-dsg3 antibody (5H10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. dsg3 protein, a member of the desmoglein family, plays a crucial role in cell adhesion by forming desmosomes, which are specialized cell junctions that provide structural integrity to tissues, particularly in the skin. This function is vital for maintaining the mechanical strength of epithelial layers, and disruption can lead to autoimmune conditions such as pemphigus vulgaris, where autoantibodies target desmogleins, resulting in blistering and loss of skin integrity. Notably, dsg3 is predominantly expressed in the basal and suprabasal layers of the epidermis, and expression is often altered in various cancers, indicating a potential role in tumor progression and metastasis. dsg3 (5H10) antibody effectively detects this protein, making dsg3 (5H10) monoclonal antibody an essential tool for researchers studying cell adhesion mechanisms, autoimmune diseases, and cancer biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
dsg3 Antibody (5H10) References:
- Antibodies against keratinocyte antigens other than desmogleins 1 and 3 can induce pemphigus vulgaris-like lesions. | Nguyen, VT., et al. 2000. J Clin Invest. 106: 1467-79. PMID: 11120754
- Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules. | Wheeler, GN., et al. 1991. Proc Natl Acad Sci U S A. 88: 4796-800. PMID: 1711210
- Autoantibodies against a novel epithelial cadherin in pemphigus vulgaris, a disease of cell adhesion. | Amagai, M., et al. 1991. Cell. 67: 869-77. PMID: 1720352
- Structural analysis and expression of human desmoglein: a cadherin-like component of the desmosome. | Nilles, LA., et al. 1991. J Cell Sci. 99 (Pt 4): 809-21. PMID: 1770008
- DSG3 facilitates cancer cell growth and invasion through the DSG3-plakoglobin-TCF/LEF-Myc/cyclin D1/MMP signaling pathway. | Chen, YJ., et al. 2013. PLoS One. 8: e64088. PMID: 23737966
- E-cadherin and Src associate with extradesmosomal Dsg3 and modulate desmosome assembly and adhesion. | Rötzer, V., et al. 2015. Cell Mol Life Sci. 72: 4885-97. PMID: 26115704
- Prognostic significance of DSG3 in rectal adenocarcinoma treated with preoperative chemoradiotherapy. | Chao, TB., et al. 2016. Future Oncol. 12: 1457-67. PMID: 27040321
- Desmoglein 3 (Dsg3) expression in cancer: A tissue microarray study on 15,869 tumors. | Viehweger, F., et al. 2022. Pathol Res Pract. 240: 154200. PMID: 36375372
- Cytoskeletal anchorage of different Dsg3 pools revealed by combination of hybrid STED/SMFS-AFM. | Fuchs, M., et al. 2023. Cell Mol Life Sci. 80: 25. PMID: 36602635
- Differences in the expression of pemphigus antigens during epidermal differentiation. | Iwatsuki, K., et al. 1995. Br J Dermatol. 133: 209-16. PMID: 7547386
- Identification of the ubiquitous human desmoglein, Dsg2, and the expression catalogue of the desmoglein subfamily of desmosomal cadherins. | Schäfer, S., et al. 1994. Exp Cell Res. 211: 391-9. PMID: 8143788
- Abnormal desmoglein expression by squamous cell carcinoma cells. | Harada, H., et al. 1996. Acta Derm Venereol. 76: 417-20. PMID: 8982400
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
dsg3 Antibody (5H10) | sc-23912 | 200 µg/ml | $322.00 | |||
dsg3 Antibody (5H10): m-IgG Fc BP-HRP Bundle | sc-526631 | 200 µg Ab; 10 µg BP | $361.00 | |||
dsg3 Antibody (5H10): m-IgG1 BP-HRP Bundle | sc-532004 | 200 µg Ab; 20 µg BP | $361.00 | |||
dsg3 Antibody (5H10) AC | sc-23912 AC | 500 µg/ml, 25% agarose | $424.00 | |||
dsg3 Antibody (5H10) HRP | sc-23912 HRP | 200 µg/ml | $322.00 | |||
dsg3 Antibody (5H10) FITC | sc-23912 FITC | 200 µg/ml | $336.00 | |||
dsg3 Antibody (5H10) PE | sc-23912 PE | 200 µg/ml | $349.00 | |||
dsg3 Antibody (5H10) Alexa Fluor® 488 | sc-23912 AF488 | 200 µg/ml | $364.00 | |||
dsg3 Antibody (5H10) Alexa Fluor® 546 | sc-23912 AF546 | 200 µg/ml | $364.00 | |||
dsg3 Antibody (5H10) Alexa Fluor® 594 | sc-23912 AF594 | 200 µg/ml | $364.00 | |||
dsg3 Antibody (5H10) Alexa Fluor® 647 | sc-23912 AF647 | 200 µg/ml | $364.00 | |||
dsg3 Antibody (5H10) Alexa Fluor® 680 | sc-23912 AF680 | 200 µg/ml | $364.00 | |||
dsg3 Antibody (5H10) Alexa Fluor® 790 | sc-23912 AF790 | 200 µg/ml | $364.00 |