dsg3 Antibody (5H10) recommended for detection of desmoglein 3 of human origin by WB, IP and IF

dsg3 Antibody (5H10): sc-23912

dsg3 Antibody (5H10) is rated 3.7 out of 5 by 3.
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Datasheets

    • Anti-dsg3 Antibody (5H10) is a mouse monoclonal IgG1 (kappa light chain) dsg3 antibody provided at 200 µg/ml
    • raised against an extracellular domain of human dsg3
    • dsg3 Antibody (5H10) is recommended for detection of desmoglein 3 of human origin by WB, IP and IF; non cross-reactive with dsg1, dsg2, or with mouse proteins
    • Anti-dsg3 Antibody (5H10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of dsg3 (5H10): sc-23912.
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What is the recommended fixation method for immunofluorescence staining with dsg3 (5H10): sc-23912 monoclonal antibody?

Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Reliable Antibody for Dsg3 Great antibody for WB of dsg3 specifically. Strong, clear signal with little to no background.
Date published: 2017-01-24
Rated 2 out of 5 by from Robust band seen in WB using SCC Robust band seen in WB using SCC-25 whole cell lysate. -SCBT QC
Date published: 2015-03-06
Rated 4 out of 5 by from Clear immunofluorescence membrane staining Clear immunofluorescence membrane staining in methanol-fixed SCC-4 cells. -SCBT QC
Date published: 2015-02-15
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