dsg2 Antibody (6D8) recommended for detection of dsg2 of human origin by WB, IP and IF

dsg2 Antibody (6D8): sc-53486

dsg2 Antibody (6D8) is rated 4.0 out of 5 by 2.
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Datasheets

    • dsg2 Antibody (6D8) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against A-431 cell shavings
    • recommended for detection of dsg2 of human origin by WB, IP and IF; non cross-reactive with Desmoglein 1 or 3
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See dsg2 (AH12.2): sc-80663 for dsg2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What is the recommended starting dilution for Western blot for sc-53486: dsg2 (6D8) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Used in IF and got good staining Used in IF and got good staining, will order again.
Date published: 2015-08-05
Rated 3 out of 5 by from Robust bands seen in WB using ECV304 Robust bands seen in WB using ECV304, T24, SCC-4, SW480, A549, JEG-3 and A-431 whole cell lysates. -SCBT QC
Date published: 2013-01-20
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