dsg1 Antibody (B-11): sc-137164

dsg1 Antibody (B-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects dsg1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-dsg1 antibody (B-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. dsg1, a member of the desmoglein family, plays a crucial role in cell adhesion by forming desmosomes, which are specialized cell junctions that provide mechanical strength to tissues, particularly in the skin. These desmosomal structures are essential for maintaining the integrity of epithelial layers, and alterations in dsg1 expression can lead to severe skin disorders, such as pemphigus foliaceus, an autoimmune disease characterized by blistering due to the presence of autoantibodies against desmogleins. dsg1′s extracellular N-terminal domain contains cadherin-like repeats that bind calcium ions, which are vital for the stability and function of desmosomes. In squamous cell carcinoma, dsg1 expression is often diminished or aberrant, indicating a potential link between desmoglein expression and tumor progression, including cell invasion and metastasis. Understanding dsg1′s role in cell adhesion and disease states highlights anti-dsg1 antibody (B-11) as a valuable tool for research in cell biology and pathology.

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dsg1 Antibody (B-11) References:

1. Antibodies against keratinocyte antigens other than desmogleins 1 and 3 can induce pemphigus vulgaris-like lesions.  |  Nguyen, VT., et al. 2000. J Clin Invest. 106: 1467-79. PMID: 11120754
2. Generation of monoclonal antibodies specific for desmoglein family members.  |  Wahl, JK. 2002. Hybrid Hybridomics. 21: 37-44. PMID: 11991815
3. Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules.  |  Wheeler, GN., et al. 1991. Proc Natl Acad Sci U S A. 88: 4796-800. PMID: 1711210
4. Autoantibodies against a novel epithelial cadherin in pemphigus vulgaris, a disease of cell adhesion.  |  Amagai, M., et al. 1991. Cell. 67: 869-77. PMID: 1720352
5. Structural analysis and expression of human desmoglein: a cadherin-like component of the desmosome.  |  Nilles, LA., et al. 1991. J Cell Sci. 99 (Pt 4): 809-21. PMID: 1770008
6. Detailed profiling of anti-desmoglein autoantibodies identifies anti-Dsg1 reactivity as a key driver of disease activity and clinical expression in pemphigus vulgaris.  |  Naseer, SY., et al. 2015. Autoimmunity. 48: 231-41. PMID: 25384535
7. Compound heterozygosity for dominant and recessive DSG1 mutations in a patient with atypical SAM syndrome (severe dermatitis, multiple allergies, metabolic wasting).  |  Dănescu, S., et al. 2017. J Eur Acad Dermatol Venereol. 31: e144-e146. PMID: 27632246
8. Novel mutation in the DSG1 gene causes autosomal-dominant striate palmoplantar keratoderma in a large Syrian family.  |  Abi Zamer, B., et al. 2019. Ann Hum Genet. 83: 472-476. PMID: 31192455
9. Differences in the expression of pemphigus antigens during epidermal differentiation.  |  Iwatsuki, K., et al. 1995. Br J Dermatol. 133: 209-16. PMID: 7547386
10. The extracellular aminoterminal domain of bovine desmoglein 1 (Dsg1) is recognized only by certain pemphigus foliaceus sera, whereas its intracellular domain is recognized by both pemphigus vulgaris and pemphigus foliaceus sera.  |  Dmochowski, M., et al. 1994. J Invest Dermatol. 103: 173-7. PMID: 8040606
11. Identification of the ubiquitous human desmoglein, Dsg2, and the expression catalogue of the desmoglein subfamily of desmosomal cadherins.  |  Schäfer, S., et al. 1994. Exp Cell Res. 211: 391-9. PMID: 8143788
12. Abnormal desmoglein expression by squamous cell carcinoma cells.  |  Harada, H., et al. 1996. Acta Derm Venereol. 76: 417-20. PMID: 8982400

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-dsg1 Antibody (B-11) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-dsg1 Antibody (B-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

dsg1 Antibody (B-11) Specifications

Alternate Product Name: Desmoglein 1

Product Clone Name: B-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 760-1049

Epitope Target: dsg1

Epitope Species: human

Product Reactivity/Detection: dsg1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for dsg1 Antibody (B-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred dsg1 antibody is dsg1 Antibody (27B2): sc-59904.