DPP7 Antibody (B-7) recommended for detection of DPP7 of mouse and rat origin by WB, IP, IF and ELISA

DPP7 Antibody (B-7): sc-514833

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Datasheets

    • DPP7 Antibody (B-7) is a mouse monoclonal mouse IgG2a κ (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 382-401 within an internal region of DPP7 of mouse origin
    • recommended for detection of DPP7 of mouse and rat origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

How much sample do you recommend loading on the gel for Western blot detection using DPP7 (B-7): sc-514833?

Asked by: Professor Griffin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-07-07
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