Dnmt3b Antibody (F-2): sc-393845

Dnmt3b Antibody (F-2) is a mouse monoclonal IgG1 antibody that detects Dnmt3b in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Dnmt3b plays a crucial role in regulating gene expression during embryonic development by catalyzing methyl group addition to cytosines within CpG dinucleotides, thereby influencing chromatin structure and gene silencing. Dnmt3b (F-2) antibody targets one of the key enzymes responsible for de novo methylation, which establishes new methylation patterns essential for cellular differentiation and development. Dnmt3b is predominantly expressed in embryonic stem cells, working alongside Dnmt3a to ensure proper DNA methylation, thus facilitating pluripotency maintenance and transition to differentiated states. Anti-Dnmt3b antibody (F-2) serves as an invaluable tool for studying gene expression and epigenetic modifications, as Dnmt3b interacts with various transcription factors and regulatory proteins in developmental biology and disease contexts.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Dnmt3b Antibody (F-2) References:

1. DNMT3B interacts with constitutive centromere protein CENP-C to modulate DNA methylation and the histone code at centromeric regions.  |  Gopalakrishnan, S., et al. 2009. Hum Mol Genet. 18: 3178-93. PMID: 19482874
2. Dnmt3b recruitment through E2F6 transcriptional repressor mediates germ-line gene silencing in murine somatic tissues.  |  Velasco, G., et al. 2010. Proc Natl Acad Sci U S A. 107: 9281-6. PMID: 20439742
3. DNMT3B overexpression by deregulation of FOXO3a-mediated transcription repression and MDM2 overexpression in lung cancer.  |  Yang, YC., et al. 2014. J Thorac Oncol. 9: 1305-15. PMID: 25122426
4. DNMT3B in vitro knocking-down is able to reverse embryonal rhabdomyosarcoma cell phenotype through inhibition of proliferation and induction of myogenic differentiation.  |  Megiorni, F., et al. 2016. Oncotarget. 7: 79342-79356. PMID: 27764816
5. Dnmt3b Methylates DNA by a Noncooperative Mechanism, and Its Activity Is Unaffected by Manipulations at the Predicted Dimer Interface.  |  Norvil, AB., et al. 2018. Biochemistry. 57: 4312-4324. PMID: 27768276
6. DNMT3B modulates the expression of cancer-related genes and downregulates the expression of the gene VAV3 via methylation.  |  Peralta-Arrieta, I., et al. 2017. Am J Cancer Res. 7: 77-87. PMID: 28123849
7. [High expression of DNMT3B promotes proliferation and invasion of hepatocellular carcinoma cells via Hippo signaling pathway].  |  Dong, G., et al. 2019. Nan Fang Yi Ke Da Xue Xue Bao. 39: 1443-1452. PMID: 31907153
8. [Expression of DNMT3b in human bladder cancer tissue and its correlation with clinical prognosis].  |  Cao, Y., et al. 2020. Nan Fang Yi Ke Da Xue Xue Bao. 40: 1295-1300. PMID: 32990224
9. Complex DNA sequence readout mechanisms of the DNMT3B DNA methyltransferase.  |  Dukatz, M., et al. 2020. Nucleic Acids Res. 48: 11495-11509. PMID: 33105482
10. DNMT3B overexpression downregulates genes with CpG islands, common motifs, and transcription factor binding sites that interact with DNMT3B.  |  Loaeza-Loaeza, J., et al. 2022. Sci Rep. 12: 20839. PMID: 36460706

The preferred Dnmt3b antibody is Dnmt3b Antibody (G-9): sc-376043.