Dnmt3b Antibody (G-9): sc-376043

Dnmt3b Antibody (G-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Dnmt3b protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Dnmt3b (G-9) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dnmt3b protein plays a crucial role in DNA methylation, a key epigenetic modification that regulates gene expression and is essential for normal development and cellular differentiation. Specifically, Dnmt3b is involved in de novo methylation, which establishes methylation patterns during embryogenesis and is vital for the silencing of transposable elements and imprinted genes. Dysregulation of Dnmt3b has been implicated in various diseases, including cancer, where abnormal methylation patterns can lead to the activation of oncogenes or silencing of tumor suppressor genes. Understanding Dnmt3b function and regulatory mechanisms is important for elucidating the complexities of gene expression and potential therapeutic targets for epigenetic interventions.

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Dnmt3b Antibody (G-9) References:

1. DNA methyltransferase is actively retained in the cytoplasm during early development.  |  Cardoso, MC. and Leonhardt, H. 1999. J Cell Biol. 147: 25-32. PMID: 10508852
2. In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b.  |  Hsieh, CL. 1999. Mol Cell Biol. 19: 8211-8. PMID: 10567546
3. DNA methyltransferase Dnmt1 associates with histone deacetylase activity.  |  Fuks, F., et al. 2000. Nat Genet. 24: 88-91. PMID: 10615135
4. Transcriptional regulation of the human DNA Methyltransferase (dnmt1) gene.  |  Bigey, P., et al. 2000. Gene. 242: 407-18. PMID: 10721735
5. Dnmt3b regulates DUX4 expression in a tissue-dependent manner in transgenic D4Z4 mice.  |  Bouwman, LF., et al. 2020. Skelet Muscle. 10: 27. PMID: 33004076
6. DNMT3B silencing suppresses migration and invasion by epigenetically promoting miR-34a in bladder cancer.  |  Xu, K., et al. 2020. Aging (Albany NY). 12: 23668-23683. PMID: 33221743
7. DNMT3B regulates proliferation of A549 cells through the microRNA-152-3p/NCAM1 pathway.  |  Yang, B., et al. 2022. Oncol Lett. 23: 11. PMID: 34820010
8. Brown Fat Dnmt3b Deficiency Ameliorates Obesity in Female Mice.  |  Li, F., et al. 2021. Life (Basel). 11: PMID: 34947856
9. PTBP1 Regulates DNMT3B Alternative Splicing by Interacting With RALY to Enhance the Radioresistance of Prostate Cancer.  |  He, H., et al. 2024. Adv Sci (Weinh). 11: e2405997. PMID: 39287090
10. DNA (cytosine-5)-methyltransferases in mouse cells and tissues. Studies with a mechanism-based probe.  |  Yoder, JA., et al. 1997. J Mol Biol. 270: 385-95. PMID: 9237905
11. Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells.  |  Okano, M., et al. 1998. Nucleic Acids Res. 26: 2536-40. PMID: 9592134
12. Cytosine methylation and mammalian development.  |  Walsh, CP. and Bestor, TH. 1999. Genes Dev. 13: 26-34. PMID: 9887097

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dnmt3b Antibody (G-9) has 35 citations in a variety of scientific publications.

Primary antibodies like Anti-Dnmt3b Antibody (G-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dnmt3b Antibody (G-9) Specifications

Alternate Product Name: DNMT

Product Clone Name: G-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-230

Epitope Target: Dnmt3b

Epitope Species: human

Product Reactivity/Detection: Dnmt3b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for Dnmt3b Antibody (G-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin