Dnmt3a Antibody (C-12): sc-365769

Dnmt3a Antibody (C-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects Dnmt3a protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-Dnmt3a antibody (C-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dnmt3a protein plays a crucial role in regulating gene expression through its function as a DNA methyltransferase, specifically catalyzing methyl group addition to the 5′-position of cytosines within CpG dinucleotide context. This methylation maintains genomic stability and regulates transcriptional repression, particularly important during embryonic development and cellular differentiation. Dnmt3a, along with its closely related enzyme Dnmt3b, shows high expression in embryonic stem cells, where Dnmt3a establishes methylation patterns that dictate cell fate and lineage commitment. Dnmt3a′s ability to modify DNA highlights its significance in epigenetic regulation and potential implications in developmental biology and disease states, including cancer, where aberrant methylation patterns can lead to dysregulated gene expression.

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Dnmt3a Antibody (C-12) References:

1. DNA methyltransferase is actively retained in the cytoplasm during early development.  |  Cardoso, MC. and Leonhardt, H. 1999. J Cell Biol. 147: 25-32. PMID: 10508852
2. In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b.  |  Hsieh, CL. 1999. Mol Cell Biol. 19: 8211-8. PMID: 10567546
3. DNA methyltransferase Dnmt1 associates with histone deacetylase activity.  |  Fuks, F., et al. 2000. Nat Genet. 24: 88-91. PMID: 10615135
4. Transcriptional regulation of the human DNA Methyltransferase (dnmt1) gene.  |  Bigey, P., et al. 2000. Gene. 242: 407-18. PMID: 10721735
5. Uterine Dnmt3a is not Required for Mouse Embryo Implantation.  |  Li, N., et al. 2020. Curr Mol Med. 20: 633-642. PMID: 32096741
6. Somatic Dnmt3a inactivation leads to slow, canonical DNA methylation loss in murine hematopoietic cells.  |  Smith, AM., et al. 2022. iScience. 25: 104004. PMID: 35313694
7. DNMT3A R882H mutation promotes acute leukemic cell survival by regulating glycolysis through the NRF2/NQO1 axis.  |  Chu, X., et al. 2023. Cell Signal. 105: 110626. PMID: 36758683
8. Loss of Dnmt3a impairs hematopoietic homeostasis and myeloid cell skewing via the PI3Kinase pathway.  |  Palam, LR., et al. 2023. JCI Insight. 8: PMID: 36976647
9. [Research progress on DNMT3A gene expression in Acute myeloid leukemia].  |  Zhou, J., et al. 2024. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 41: 1010-1015. PMID: 39097288
10. DNA (cytosine-5)-methyltransferases in mouse cells and tissues. Studies with a mechanism-based probe.  |  Yoder, JA., et al. 1997. J Mol Biol. 270: 385-95. PMID: 9237905
11. Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells.  |  Okano, M., et al. 1998. Nucleic Acids Res. 26: 2536-40. PMID: 9592134
12. Cytosine methylation and mammalian development.  |  Walsh, CP. and Bestor, TH. 1999. Genes Dev. 13: 26-34. PMID: 9887097

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Dnmt3a Antibody (C-12) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-Dnmt3a Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Dnmt3a Antibody (C-12) Specifications

Alternate Product Name: DNMT

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 42-67

Epitope Target: Dnmt3a

Epitope Species: human

Product Reactivity/Detection: Dnmt3a

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for Dnmt3a Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin