Dnmt3a Antibody (C-12): sc-365769
- Dnmt3a Antibody (C-12) is a mouse monoclonal IgG2b κ Dnmt3a antibody, cited in 24 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 42-67 near the N-terminus of Dnmt3a of human origin
- Dnmt3a Antibody (C-12) is recommended for detection of Dnmt3a of mouse, rat and human origin by WB, IP and ELISA
- Anti-Dnmt3a Antibody (C-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Dnmt3a Antibody (C-12) for WB applications. These reagents are now offered in bundles with Dnmt3a Antibody (C-12) (see ordering information below).
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Dnmt3a Antibody (C-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects Dnmt3a protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). anti-Dnmt3a antibody (C-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dnmt3a protein plays a crucial role in regulating gene expression through its function as a DNA methyltransferase, specifically catalyzing methyl group addition to the 5′-position of cytosines within CpG dinucleotide context. This methylation maintains genomic stability and regulates transcriptional repression, particularly important during embryonic development and cellular differentiation. Dnmt3a, along with its closely related enzyme Dnmt3b, shows high expression in embryonic stem cells, where Dnmt3a establishes methylation patterns that dictate cell fate and lineage commitment. Dnmt3a′s ability to modify DNA highlights its significance in epigenetic regulation and potential implications in developmental biology and disease states, including cancer, where aberrant methylation patterns can lead to dysregulated gene expression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Dnmt3a Antibody (C-12) References:
- DNA methyltransferase is actively retained in the cytoplasm during early development. | Cardoso, MC. and Leonhardt, H. 1999. J Cell Biol. 147: 25-32. PMID: 10508852
- In vivo activity of murine de novo methyltransferases, Dnmt3a and Dnmt3b. | Hsieh, CL. 1999. Mol Cell Biol. 19: 8211-8. PMID: 10567546
- DNA methyltransferase Dnmt1 associates with histone deacetylase activity. | Fuks, F., et al. 2000. Nat Genet. 24: 88-91. PMID: 10615135
- Transcriptional regulation of the human DNA Methyltransferase (dnmt1) gene. | Bigey, P., et al. 2000. Gene. 242: 407-18. PMID: 10721735
- Uterine Dnmt3a is not Required for Mouse Embryo Implantation. | Li, N., et al. 2020. Curr Mol Med. 20: 633-642. PMID: 32096741
- Somatic Dnmt3a inactivation leads to slow, canonical DNA methylation loss in murine hematopoietic cells. | Smith, AM., et al. 2022. iScience. 25: 104004. PMID: 35313694
- DNMT3A R882H mutation promotes acute leukemic cell survival by regulating glycolysis through the NRF2/NQO1 axis. | Chu, X., et al. 2023. Cell Signal. 105: 110626. PMID: 36758683
- Loss of Dnmt3a impairs hematopoietic homeostasis and myeloid cell skewing via the PI3Kinase pathway. | Palam, LR., et al. 2023. JCI Insight. 8: PMID: 36976647
- [Research progress on DNMT3A gene expression in Acute myeloid leukemia]. | Zhou, J., et al. 2024. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 41: 1010-1015. PMID: 39097288
- DNA (cytosine-5)-methyltransferases in mouse cells and tissues. Studies with a mechanism-based probe. | Yoder, JA., et al. 1997. J Mol Biol. 270: 385-95. PMID: 9237905
- Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells. | Okano, M., et al. 1998. Nucleic Acids Res. 26: 2536-40. PMID: 9592134
- Cytosine methylation and mammalian development. | Walsh, CP. and Bestor, TH. 1999. Genes Dev. 13: 26-34. PMID: 9887097
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dnmt3a Antibody (C-12) | sc-365769 | 200 µg/ml | $322.00 | |||
Dnmt3a Antibody (C-12): m-IgG Fc BP-HRP Bundle | sc-529460 | 200 µg Ab; 10 µg BP | $361.00 | |||
Dnmt3a Antibody (C-12): m-IgGκ BP-HRP Bundle | sc-522358 | 200 µg Ab, 40 µg BP | $361.00 | |||
Dnmt3a Antibody (C-12): m-IgG2b BP-HRP Bundle | sc-550114 | 200 µg Ab; 10 µg BP | $361.00 | |||
Dnmt3a Antibody (C-12) AC | sc-365769 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Dnmt3a Antibody (C-12) HRP | sc-365769 HRP | 200 µg/ml | $322.00 | |||
Dnmt3a Antibody (C-12) FITC | sc-365769 FITC | 200 µg/ml | $336.00 | |||
Dnmt3a Antibody (C-12) PE | sc-365769 PE | 200 µg/ml | $349.00 | |||
Dnmt3a Antibody (C-12) Alexa Fluor® 488 | sc-365769 AF488 | 200 µg/ml | $364.00 | |||
Dnmt3a Antibody (C-12) Alexa Fluor® 546 | sc-365769 AF546 | 200 µg/ml | $364.00 | |||
Dnmt3a Antibody (C-12) Alexa Fluor® 594 | sc-365769 AF594 | 200 µg/ml | $364.00 | |||
Dnmt3a Antibody (C-12) Alexa Fluor® 647 | sc-365769 AF647 | 200 µg/ml | $364.00 | |||
Dnmt3a Antibody (C-12) Alexa Fluor® 680 | sc-365769 AF680 | 200 µg/ml | $364.00 | |||
Dnmt3a Antibody (C-12) Alexa Fluor® 790 | sc-365769 AF790 | 200 µg/ml | $364.00 | |||
Dnmt3a (C-12) Neutralizing Peptide | sc-365769 P | 100 µg/0.5 ml | $69.00 |