DNase I Antibody (F-2): sc-515205

DNase I Antibody (F-2) is a mouse monoclonal IgG1 antibody that detects DNase I in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNase I plays a crucial role in the apoptotic process by mediating internucleosomal DNA fragmentation, which is a hallmark of programmed cell death. This enzyme is a calcium and magnesium-dependent endonuclease that is ubiquitously expressed across mammalian tissues, highlighting its importance in cellular homeostasis and the regulation of apoptosis. Notably, DNase I is highly polymorphic, with at least six known alleles, which may influence its activity and function in different individuals. DNase I is primarily localized in the nuclei of epithelial and fiber cells, where DNase I cleaves DNA into 5′-phosphodinucleotide and 5′-phospho-oligonucleotide end-products, facilitating the orderly degradation of genomic DNA during apoptosis. Understanding the function and regulation of DNase I is essential, as dysregulation of this enzyme can lead to various pathological conditions, including autoimmune diseases and cancer. Anti-DNase I antibody (F-2) provides a valuable tool for researchers studying the mechanisms of apoptosis and the role of nucleases in cellular processes.

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DNase I Antibody (F-2) References:

1. DNase I behaves as a transcription factor which modulates Fas expression in human cells.  |  Oliveri, M., et al. 2004. Eur J Immunol. 34: 273-9. PMID: 14971053
2. How cations can assist DNase I in DNA binding and hydrolysis.  |  Guéroult, M., et al. 2010. PLoS Comput Biol. 6: e1001000. PMID: 21124947
3. Differential DNase I hypersensitivity reveals factor-dependent chromatin dynamics.  |  He, HH., et al. 2012. Genome Res. 22: 1015-25. PMID: 22508765
4. Antitumor Effects of Systemic DNAse I and Proteases in an In Vivo Model.  |  Trejo-Becerril, C., et al. 2016. Integr Cancer Ther. 15: NP35-NP43. PMID: 27146129
5. Identifying DNase I hypersensitive sites as driver distal regulatory elements in breast cancer.  |  D Antonio, M., et al. 2017. Nat Commun. 8: 436. PMID: 28874753
6. Deep learning for DNase I hypersensitive sites identification.  |  Lyu, C., et al. 2018. BMC Genomics. 19: 905. PMID: 30598079
7. Genome-Wide Characterization of DNase I-Hypersensitive Sites and Cold Response Regulatory Landscapes in Grasses.  |  Han, J., et al. 2020. Plant Cell. 32: 2457-2473. PMID: 32471863
8. Extracellular G-quadruplexes and Z-DNA protect biofilms from DNase I, and G-quadruplexes form a DNAzyme with peroxidase activity.  |  Minero, GAS., et al. 2024. Nucleic Acids Res. 52: 1575-1590. PMID: 38296834
9. Phalloidin and DNase I-bound F-actin pointed end structures reveal principles of filament stabilization and disassembly.  |  Boiero Sanders, M., et al. 2024. Nat Commun. 15: 7969. PMID: 39261469
10. The dependence of DNase I activity on the conformation of oligodeoxynucleotides.  |  Sutton, DH., et al. 1997. Biochem J. 321 (Pt 2): 481-6. PMID: 9020884

The preferred DNase I antibody is DNase I Antibody (B-4): sc-376207.