DNase I Antibody (B-4): sc-376207

DNase I Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects DNase I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). DNase I (B-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DNase I plays a crucial role in apoptosis by mediating internucleosomal DNA fragmentation, which is a hallmark of programmed cell death. This Ca2+/Mg2+-dependent endonuclease is widely expressed across mammalian tissues and is essential for the degradation of DNA in cells undergoing apoptosis, ensuring the orderly dismantling of cellular components. DNase I is highly polymorphic, with at least six known alleles, and DNase I activity is complemented by DNase II, which operates downstream of caspase activation and also contributes to DNA degradation during apoptosis. While DNase I is predominantly localized in the nuclei of epithelial and fiber cells, DNase II is typically found in the cytoplasm of epithelial cells, with a notable concentration in the nuclei of lens fibers. Understanding the distinct roles and localization of these enzymes is vital for elucidating the mechanisms of apoptosis and their implications in various diseases, including cancer.

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DNase I Antibody (B-4) References:

1. A new allele, DNASE1*6, of human deoxyribonuclease I polymorphism encodes an Arg to Cys substitution responsible for its instability.  |  Yasuda, T., et al. 1999. Biochem Biophys Res Commun. 260: 280-3. PMID: 10381379
2. Characterization of human DNase I family endonucleases and activation of DNase gamma during apoptosis.  |  Shiokawa, D. and Tanuma, S. 2001. Biochemistry. 40: 143-52. PMID: 11141064
3. DNase I mediates internucleosomal DNA degradation in human cells undergoing drug-induced apoptosis.  |  Oliveri, M., et al. 2001. Eur J Immunol. 31: 743-51. PMID: 11241278
4. Increased DNase I activity in diabetes might be associated with injury of pancreas.  |  Zhu, B., et al. 2014. Mol Cell Biochem. 393: 23-32. PMID: 24676545
5. Genome-wide prediction of DNase I hypersensitivity using gene expression.  |  Zhou, W., et al. 2017. Nat Commun. 8: 1038. PMID: 29051481
6. Ascorbic acid as DNase I inhibitor in prevention of male infertility.  |  Ilić, BS., et al. 2018. Biochem Biophys Res Commun. 498: 1073-1077. PMID: 29551685
7. Synthesis and DNase I inhibitory properties of some 4-thiazolidinone derivatives.  |  Kolarević, A., et al. 2019. J Cell Biochem. 120: 264-274. PMID: 30171712
8. Bioinspired DNase-I-Coated Melanin-Like Nanospheres for Modulation of Infection-Associated NETosis Dysregulation.  |  Park, HH., et al. 2021. Adv Sci (Weinh). 8: e2103748. PMID: 34623037
9. Involvement of DNase II in nuclear degeneration during lens cell differentiation.  |  Torriglia, A., et al. 1995. J Biol Chem. 270: 28579-85. PMID: 7499373
10. Molecular cloning of the cDNA encoding human deoxyribonuclease II.  |  Yasuda, T., et al. 1998. J Biol Chem. 273: 2610-6. PMID: 9446563
11. Molecular cloning and characterization of human and murine DNase II.  |  Baker, KP., et al. 1998. Gene. 215: 281-9. PMID: 9714827
12. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis.  |  Krieser, RJ. and Eastman, A. 1998. J Biol Chem. 273: 30909-14. PMID: 9812984

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNase I Antibody (B-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-DNase I Antibody (B-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNase I Antibody (B-4) Specifications

Alternate Product Name: DNase I

Product Clone Name: B-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 73-91

Epitope Target: DNase I

Epitope Species: human

Product Reactivity/Detection: DNase I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for DNase I Antibody (B-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin