DNA pol α Antibody (D-7): sc-137021
- DNA pol α Antibody (D-7) is a mouse monoclonal IgG1 κ DNA pol α antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1211-1440 mapping near the C-terminus of DNA pol α of human origin
- DNA pol alpha Antibody (D-7) is recommended for detection of DNA pol α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-DNA pol alpha Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DNA pol α Antibody (D-7) for WB and IHC(P) applications. These reagents are now offered in bundles with DNA pol α Antibody (D-7) (see ordering information below).
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DNA pol α Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects DNA pol alpha (DNA pol α) protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-DNA pol α antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DNA pol α protein plays a crucial role in DNA replication, synthesizing the RNA-DNA primer necessary for initiating DNA strand synthesis. This function maintains genomic stability, as replication errors can lead to mutations and genomic instability, which are hallmarks of many diseases, including cancer. DNA pol α belongs to a larger family of DNA polymerases working collaboratively to ensure accurate DNA replication and repair. DNA pol α interaction with other polymerases, such as DNA pol δ and ε, coordinates activities to manage DNA synthesis and repair, maintaining genome integrity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DNA pol α Antibody (D-7) References:
- Eukaryotic DNA mismatch repair. | Kolodner, RD. and Marsischky, GT. 1999. Curr Opin Genet Dev. 9: 89-96. PMID: 10072354
- A full-length cDNA of hREV3 is predicted to encode DNA polymerase zeta for damage-induced mutagenesis in humans. | Lin, W., et al. 1999. Mutat Res. 433: 89-98. PMID: 10102035
- DNA repair. Variants on a theme. | Wood, RD. 1999. Nature. 399: 639-40. PMID: 10385109
- Cloning and chromosomal mapping of the human DNA polymerase theta (POLQ), the eighth human DNA polymerase. | Sharief, FS., et al. 1999. Genomics. 59: 90-6. PMID: 10395804
- Telomerase-mediated telomere addition in vivo requires DNA primase and DNA polymerases alpha and delta. | Diede, SJ. and Gottschling, DE. 1999. Cell. 99: 723-33. PMID: 10619426
- DNA replication. Eukaryotic initiation rites. | Li, JJ. and Alberts, BM. 1992. Nature. 357: 114-5. PMID: 1579160
- Properties of DNA polymerases delta and epsilon, and their roles in eukaryotic DNA replication. | Bambara, RA. and Jessee, CB. 1991. Biochim Biophys Acta. 1088: 11-24. PMID: 1846563
- Human DNA polymerase α in binary complex with a DNA:DNA template-primer. | Coloma, J., et al. 2016. Sci Rep. 6: 23784. PMID: 27032819
- Human DNA polymerase α interacts with mismatch repair proteins MSH2 and MSH6. | Itkonen, HM., et al. 2016. FEBS Lett. 590: 4233-4241. PMID: 27805738
- DNA polymerase α interacts with H3-H4 and facilitates the transfer of parental histones to lagging strands. | Li, Z., et al. 2020. Sci Adv. 6: eabb5820. PMID: 32923642
- Human DNA polymerase α has a strong mutagenic potential at the initial steps of DNA synthesis. | Lisova, AE., et al. 2022. Nucleic Acids Res. 50: 12266-12273. PMID: 36454017
- Cloning and characterization of the human mitochondrial DNA polymerase, DNA polymerase gamma. | Ropp, PA. and Copeland, WC. 1996. Genomics. 36: 449-58. PMID: 8884268
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DNA pol α Antibody (D-7) | sc-137021 | 200 µg/ml | $322.00 | |||
DNA pol α Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-528839 | 200 µg Ab; 10 µg BP | $361.00 | |||
DNA pol α Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-521397 | 200 µg Ab, 40 µg BP | $361.00 | |||
DNA pol α Antibody (D-7): m-IgG1 BP-HRP Bundle | sc-543163 | 200 µg Ab; 20 µg BP | $361.00 | |||
DNA pol α Antibody (D-7) AC | sc-137021 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DNA pol α Antibody (D-7) HRP | sc-137021 HRP | 200 µg/ml | $322.00 | |||
DNA pol α Antibody (D-7) FITC | sc-137021 FITC | 200 µg/ml | $336.00 | |||
DNA pol α Antibody (D-7) PE | sc-137021 PE | 200 µg/ml | $349.00 | |||
DNA pol α Antibody (D-7) Alexa Fluor® 488 | sc-137021 AF488 | 200 µg/ml | $364.00 | |||
DNA pol α Antibody (D-7) Alexa Fluor® 546 | sc-137021 AF546 | 200 µg/ml | $364.00 | |||
DNA pol α Antibody (D-7) Alexa Fluor® 594 | sc-137021 AF594 | 200 µg/ml | $364.00 | |||
DNA pol α Antibody (D-7) Alexa Fluor® 647 | sc-137021 AF647 | 200 µg/ml | $364.00 | |||
DNA pol α Antibody (D-7) Alexa Fluor® 680 | sc-137021 AF680 | 200 µg/ml | $364.00 | |||
DNA pol α Antibody (D-7) Alexa Fluor® 790 | sc-137021 AF790 | 200 µg/ml | $364.00 |