DNA Ligase III Antibody (E-7): sc-390922

DNA Ligase III Antibody (E-7) is an IgG_2a κ mouse monoclonal DNA Ligase III antibody (also designated DNA Ligase III antibody) that detects the DNA Ligase III protein of human origin by WB, IP, IF and ELISA. DNA Ligase III Antibody (E-7) is available as both the non-conjugated anti-DNA Ligase III antibody form, as well as multiple conjugated forms of anti-DNA Ligase III antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. DNA ligase is a type of ligase that can link together DNA strands that have double-strand breaks. DNA ligase functions in both DNA repair and DNA replication. It is utilized in molecular biology laboratories for recombination experiments. In mammals, the four specific types of DNA ligase are known as DNA Ligase I, II, III and IV. DNA Ligase I ligates Okazaki fragments during lagging strand DNA replication and some recombinant fragments. DNA Ligase II is an alternatively spliced form of DNA Ligase III found in non-dividing cells. DNA ligase III complexes with the DNA repair protein XRCC1 to function in sealing base excision mutations and recombinant fragments. DNA Ligase IV complexes with XRCC4 and catalyzes the final step in the non-homologous end joining DNA double-strand break repair pathway.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DNA Ligase III Antibody (E-7) References:

1. The human DNA ligase III gene encodes nuclear and mitochondrial proteins.  |  Lakshmipathy, U. and Campbell, C. 1999. Mol Cell Biol. 19: 3869-76. PMID: 10207110
2. DNA ligase III as a candidate component of backup pathways of nonhomologous end joining.  |  Wang, H., et al. 2005. Cancer Res. 65: 4020-30. PMID: 15899791
3. Human DNA ligase III recognizes DNA ends by dynamic switching between two DNA-bound states.  |  Cotner-Gohara, E., et al. 2010. Biochemistry. 49: 6165-76. PMID: 20518483
4. Crucial role for DNA ligase III in mitochondria but not in Xrcc1-dependent repair.  |  Simsek, D., et al. 2011. Nature. 471: 245-8. PMID: 21390132
5. DNA ligase III promotes alternative nonhomologous end-joining during chromosomal translocation formation.  |  Simsek, D., et al. 2011. PLoS Genet. 7: e1002080. PMID: 21655080
6. Involvement of DNA ligase III and ribonuclease H1 in mitochondrial DNA replication in cultured human cells.  |  Ruhanen, H., et al. 2011. Biochim Biophys Acta. 1813: 2000-7. PMID: 21878356
7. DNA ligase III and DNA ligase IV carry out genetically distinct forms of end joining in human somatic cells.  |  Oh, S., et al. 2014. DNA Repair (Amst). 21: 97-110. PMID: 24837021
8. miR-22 suppresses DNA ligase III addiction in multiple myeloma.  |  Caracciolo, D., et al. 2019. Leukemia. 33: 487-498. PMID: 30120376
9. Loss of nuclear DNA ligase III reverts PARP inhibitor resistance in BRCA1/53BP1 double-deficient cells by exposing ssDNA gaps.  |  Paes Dias, M., et al. 2021. Mol Cell. 81: 4692-4708.e9. PMID: 34555355
10. Role of the DNA ligase III zinc finger in polynucleotide binding and ligation.  |  Taylor, RM., et al. 1998. Nucleic Acids Res. 26: 4804-10. PMID: 9776738

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA Ligase III Antibody (E-7) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA Ligase III Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA Ligase III Antibody (E-7) Specifications

Alternate Product Name: DNA Ligase III

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 181-480

Epitope Target: DNA Ligase III

Epitope Species: human

Product Reactivity/Detection: DNA Ligase III

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for DNA Ligase III Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred DNA Ligase III antibody is DNA Ligase III Antibody (7): sc-135883.