Anti-DNA Ligase III Antibody (E-7) is a mouse monoclonal IgG2a (kappa light chain) DNA Ligase III antibody provided at 200 µg/ml
raised against amino acids 181-480 mapping near the N-terminus of DNA Ligase III of human origin
DNA Ligase III Antibody (E-7) is recommended for detection of DNA Ligase III of human origin by WB, IP, IF and ELISA
Anti-DNA Ligase III Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DNA Ligase III (E-7): sc-390922.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
DNA Ligase III Antibody (E-7) is a high quality monoclonal DNA Ligase III antibody (also designated DNA Ligase III antibody) suitable for the detection of the DNA Ligase III protein of human origin. DNA Ligase III Antibody (E-7) is available as both the non-conjugated anti-DNA Ligase III antibody form, as well as multiple conjugated forms of anti-DNA Ligase III antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. DNA ligase is a type of ligase that can link together DNA strands that have double-strand breaks. DNA ligase functions in both DNA repair and DNA replication. It is utilized in molecular biology laboratories for recombination experiments. In mammals, the four specific types of DNA ligase are known as DNA Ligase I, II, III and IV. DNA Ligase I ligates Okazaki fragments during lagging strand DNA replication and some recombinant fragments. DNA Ligase II is an alternatively spliced form of DNA Ligase III found in non-dividing cells. DNA ligase III complexes with the DNA repair protein XRCC1 to function in sealing base excision mutations and recombinant fragments. DNA Ligase IV complexes with XRCC4 and catalyzes the final step in the non-homologous end joining DNA double-strand break repair pathway.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What is the recommended starting dilution for Western blot for sc-390922: DNA Ligase III (E-7) monoclonal antibody?
Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol:
Rated 4 out of
Good antibodyThis antibody performed well in our experiment. Clear band.
Date published: 2017-11-26
Rated 3 out of
Work OK for western blotUsed on cell lysate prepared from MEFs. Could detect Lig3 although the signal was not very strong.
Date published: 2017-10-19
Rated 5 out of
Good antibodyThis antibody works good at 1:500 dilution, the band looks nice.
Date published: 2016-12-01
Rated 5 out of
Very goodThe antibody has a strong band,and I will try to buy it again.
Date published: 2016-11-24
Rated 5 out of
Great Western blot data of DNA Ligase IIIGreat Western blot data of DNA Ligase III expression in non-transfected and human DNA Ligase III transfected 293T whole cell lysates. -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn