DNA Ligase III Antibody (E-7) recommended for detection of DNA Ligase III of human origin by WB, IP, IF and ELISA

DNA Ligase III Antibody (E-7): sc-390922

DNA Ligase III Antibody (E-7) is rated 4.4 out of 5 by 5.
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Datasheets

    • Anti-DNA Ligase III Antibody (E-7) is a mouse monoclonal IgG2a (kappa light chain) DNA Ligase III antibody provided at 200 µg/ml
    • raised against amino acids 181-480 mapping near the N-terminus of DNA Ligase III of human origin
    • DNA Ligase III Antibody (E-7) is recommended for detection of DNA Ligase III of human origin by WB, IP, IF and ELISA
    • Anti-DNA Ligase III Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of DNA Ligase III (E-7): sc-390922.

What is the recommended starting dilution for Western blot for sc-390922: DNA Ligase III (E-7) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Good antibody This antibody performed well in our experiment. Clear band.
Date published: 2017-11-26
Rated 3 out of 5 by from Work OK for western blot Used on cell lysate prepared from MEFs. Could detect Lig3 although the signal was not very strong.
Date published: 2017-10-19
Rated 5 out of 5 by from Good antibody This antibody works good at 1:500 dilution, the band looks nice.
Date published: 2016-12-01
Rated 5 out of 5 by from Very good The antibody has a strong band,and I will try to buy it again.
Date published: 2016-11-24
Rated 5 out of 5 by from Great Western blot data of DNA Ligase III Great Western blot data of DNA Ligase III expression in non-transfected and human DNA Ligase III transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-03-11
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