DNA Ligase III Antibody (7): sc-135883

DNA Ligase III Antibody (7) is an IgG1 mouse monoclonal DNA Ligase III antibody that detects DNA Ligase III of mouse, rat and human origin by WB and IP. DNA Ligase III Antibody (7) is available as both conjugated and non-conjugated anti-DNA Ligase III antibody forms. DNA ligase is a type of ligase that can link together DNA strands that have double-strand breaks. DNA ligase functions in both DNA repair and DNA replication. It is utilized in molecular biology laboratories for recombination experiments. In mammals, the four specific types of DNA ligase are known as DNA Ligase I, II, III and IV. DNA Ligase I ligates Okazaki fragments during lagging strand DNA replication and some recombinant fragments. DNA Ligase II is an alternatively spliced form of DNA Ligase III found in non-dividing cells. DNA ligase III complexes with the DNA repair protein XRCC1 to function in sealing base excision mutations and recombinant fragments. DNA Ligase IV complexes with XRCC4 and catalyzes the final step in the non-homologous end joining DNA double-strand break repair pathway.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

DNA Ligase III Antibody (7) References:

1. A novel interaction between DNA ligase III and DNA polymerase gamma plays an essential role in mitochondrial DNA stability.  |  De, A. and Campbell, C. 2007. Biochem J. 402: 175-86. PMID: 17054425
2. NEIL1 is the major DNA glycosylase that processes 5-hydroxyuracil in the proximity of a DNA single-strand break.  |  Parsons, JL., et al. 2007. Biochemistry. 46: 4158-63. PMID: 17348689
3. Alternative Okazaki Fragment Ligation Pathway by DNA Ligase III.  |  Arakawa, H. and Iliakis, G. 2015. Genes (Basel). 6: 385-98. PMID: 26110316
4. Human DNA ligase III bridges two DNA ends to promote specific intermolecular DNA end joining.  |  Kukshal, V., et al. 2015. Nucleic Acids Res. 43: 7021-31. PMID: 26130724
5. Exploiting DNA Ligase III addiction of multiple myeloma by flavonoid Rhamnetin.  |  Caracciolo, D., et al. 2022. J Transl Med. 20: 482. PMID: 36273153
6. DNA ligase: structure, mechanism, and function.  |  Lehman, IR. 1974. Science. 186: 790-7. PMID: 4377758
7. Mammalian DNA ligase III: molecular cloning, chromosomal localization, and expression in spermatocytes undergoing meiotic recombination.  |  Chen, J., et al. 1995. Mol Cell Biol. 15: 5412-22. PMID: 7565692
8. Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination.  |  Wei, YF., et al. 1995. Mol Cell Biol. 15: 3206-16. PMID: 7760816
9. An interaction between the mammalian DNA repair protein XRCC1 and DNA ligase III.  |  Caldecott, KW., et al. 1994. Mol Cell Biol. 14: 68-76. PMID: 8264637
10. XRCC1 polypeptide interacts with DNA polymerase beta and possibly poly (ADP-ribose) polymerase, and DNA ligase III is a novel molecular 'nick-sensor' in vitro.  |  Caldecott, KW., et al. 1996. Nucleic Acids Res. 24: 4387-94. PMID: 8948628
11. Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells.  |  Grawunder, U., et al. 1997. Nature. 388: 492-5. PMID: 9242410
12. Yeast DNA ligase IV mediates non-homologous DNA end joining.  |  Wilson, TE., et al. 1997. Nature. 388: 495-8. PMID: 9242411

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA Ligase III Antibody (7) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA Ligase III Antibody (7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA Ligase III Antibody (7) Specifications

Alternate Product Name: DNA Ligase III

Product Clone Name: 7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 2-115

Epitope Target: DNA Ligase III

Epitope Species: human

Product Reactivity/Detection: DNA Ligase III

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for DNA Ligase III Antibody (7): AC: Agarose, B: Biotin, HRP: Horseradish Peroxidase

The preferred DNA Ligase III antibody is DNA Ligase III Antibody (E-7): sc-390922.