DNA Ligase I Antibody (A-5): sc-390235

DNA Ligase I Antibody (A-5) is a mouse monoclonal IgG2a antibody that detects DNA Ligase I (DNA LIG1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DNA Ligase I is crucial for maintaining genome integrity, playing a vital role in DNA replication and repair by catalyzing phosphodiester bond formation between adjacent 5′ phosphoryl and 3′ hydroxy termini at single breaks in duplex DNA molecules. This function is particularly important during Okazaki fragment joining on the lagging strand during DNA replication, ensuring accurate and efficient genetic material replication. Additionally, DNA Ligase I seals nicks during excision repair, essential for correcting DNA damage that can lead to mutations and genomic instability. Notably, elevated DNA Ligase I levels have been observed in various human tumors compared to benign tissues, indicating potential as a biomarker for cancer progression and therapeutic intervention target. Furthermore, DNA Ligase I activity alterations are associated with Bloom′s syndrome, a chromosomal breakage disorder, highlighting DNA Ligase I′s importance in maintaining genomic stability. anti-DNA Ligase I antibody (A-5) serves as an invaluable tool for researchers studying DNA repair mechanisms and cancer biology.

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DNA Ligase I Antibody (A-5) References:

1. Mammalian DNA ligases. Catalytic domain and size of DNA ligase I.  |  Tomkinson, AE., et al. 1990. J Biol Chem. 265: 12611-7. PMID: 1695631
2. DNA ligase I deficiency leads to replication-dependent DNA damage and impacts cell morphology without blocking cell cycle progression.  |  Soza, S., et al. 2009. Mol Cell Biol. 29: 2032-41. PMID: 19223467
3. Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency.  |  Taylor, MR., et al. 2011. J Biol Chem. 286: 23054-62. PMID: 21561855
4. Human DNA ligase i (ligi) gene and risk of cervical cancer in North Indian women.  |  Kaur, S. 2013. Exp Oncol. 35: 226-8. PMID: 24084463
5. Tricyclic dihydrobenzoxazepine and tetracyclic indole derivatives can specifically target bacterial DNA ligases and can distinguish them from human DNA ligase I.  |  Yadav, N., et al. 2015. Org Biomol Chem. 13: 5475-87. PMID: 25875403
6. siRNA Delivery Using a Cationic-Lipid-Based Highly Selective Human DNA Ligase I Inhibitor.  |  Bathula, SR., et al. 2018. ACS Appl Mater Interfaces. 10: 1616-1622. PMID: 29256581
7. DNA ligase I is associated with the 21 S complex of enzymes for DNA synthesis in HeLa cells.  |  Li, C., et al. 1994. Nucleic Acids Res. 22: 632-8. PMID: 8127711
8. Normal V(D)J coding junction formation in DNA ligase I deficiency syndromes.  |  Petrini, JH., et al. 1994. J Immunol. 152: 176-83. PMID: 8254190
9. Natural-product inhibitors of human DNA ligase I.  |  Tan, GT., et al. 1996. Biochem J. 314 (Pt 3): 993-1000. PMID: 8615799
10. Inhibition of human DNA ligase I activity by zinc and cadmium and the fidelity of ligation.  |  Yang, SW., et al. 1996. Environ Mol Mutagen. 28: 19-25. PMID: 8698042

The preferred DNA Ligase I antibody is DNA Ligase I Antibody (C-5): sc-271678.