DNA Ligase I Antibody (C-5): sc-271678

DNA Ligase I Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects DNA Ligase I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-DNA Ligase I antibody (C-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DNA Ligase I (C-5) antibody recognizes a protein that plays a crucial role in DNA replication and repair, primarily by joining Okazaki fragments during lagging strand synthesis, which is essential for maintaining genomic stability in proliferating cells. DNA Ligase I (C-5) monoclonal antibody detects a protein involved in sealing nicks during excision repair, ensuring DNA molecule integrity. DNA Ligase I′s structure is characterized by a common catalytic core, which is vital for ligase activity. Elevated levels of DNA Ligase I have been observed in various human tumors compared to benign tissues, indicating potential as a biomarker for cancer progression. DNA Ligase I activity is altered in Bloom′s syndrome, a chromosomal breakage disorder, where individuals may exhibit decreased levels of a thermolabile form of the enzyme or a dimeric variant. This highlights DNA Ligase I′s importance in normal cellular processes and disease, making DNA Ligase I (C-5) antibody valuable for research in cancer biology and genetic disorders.

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DNA Ligase I Antibody (C-5) References:

1. DNA ligases in the repair and replication of DNA.  |  Timson, DJ., et al. 2000. Mutat Res. 460: 301-18. PMID: 10946235
2. Human DNA ligase I efficiently seals nicks in nucleosomes.  |  Chafin, DR., et al. 2000. EMBO J. 19: 5492-501. PMID: 11032816
3. DNA ligase I and proliferating cell nuclear antigen form a functional complex.  |  Tom, S., et al. 2001. J Biol Chem. 276: 24817-25. PMID: 11331287
4. Elevated expression of DNA ligase I in human cancers.  |  Sun, D., et al. 2001. Clin Cancer Res. 7: 4143-8. PMID: 11751514
5. Mutations in the DNA ligase I gene of an individual with immunodeficiencies and cellular hypersensitivity to DNA-damaging agents.  |  Barnes, DE., et al. 1992. Cell. 69: 495-503. PMID: 1581963
6. Mechanism of stimulation of human DNA ligase I by the Rad9-rad1-Hus1 checkpoint complex.  |  Wang, W., et al. 2006. J Biol Chem. 281: 20865-20872. PMID: 16731526
7. A wild-type DNA ligase I gene is expressed in Bloom's syndrome cells.  |  Petrini, JH., et al. 1991. Proc Natl Acad Sci U S A. 88: 7615-9. PMID: 1881902
8. Human DNA ligase I cDNA: cloning and functional expression in Saccharomyces cerevisiae.  |  Barnes, DE., et al. 1990. Proc Natl Acad Sci U S A. 87: 6679-83. PMID: 2204063
9. Human DNA Ligase I Interacts with and Is Targeted for Degradation by the DCAF7 Specificity Factor of the Cul4-DDB1 Ubiquitin Ligase Complex.  |  Peng, Z., et al. 2016. J Biol Chem. 291: 21893-21902. PMID: 27573245
10. Unchanged PCNA and DNMT1 dynamics during replication in DNA ligase I-deficient cells but abnormal chromatin levels of non-replicative histone H1.  |  Bhandari, SK., et al. 2023. Sci Rep. 13: 4363. PMID: 36928068
11. Synergy between human DNA ligase I and topoisomerase 1 unveils new therapeutic strategy for the management of colorectal cancer.  |  Maurya, P., et al. 2025. J Biomol Struct Dyn. 43: 3390-3405. PMID: 38179981
12. DNA ligase I is recruited to sites of DNA replication by an interaction with proliferating cell nuclear antigen: identification of a common targeting mechanism for the assembly of replication factories.  |  Montecucco, A., et al. 1998. EMBO J. 17: 3786-95. PMID: 9649448

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DNA Ligase I Antibody (C-5) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-DNA Ligase I Antibody (C-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DNA Ligase I Antibody (C-5) Specifications

Alternate Product Name: DNA Ligase I

Product Clone Name: C-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 3-21

Epitope Target: DNA Ligase I

Epitope Species: human

Product Reactivity/Detection: DNA Ligase I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for DNA Ligase I Antibody (C-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin