DMGDH Antibody (C-9): sc-398257

DMGDH Antibody (C-9) is a mouse monoclonal IgG1 antibody that detects DMGDH (dimethylglycine dehydrogenase) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DMGDH is an 866 amino acid mitochondrial protein that plays a crucial role in choline catabolism by catalyzing the demethylation of dimethylglycine to form sarcosine, a process vital for maintaining proper metabolic function. DMGDH exists as a monomer and belongs to the gcvT family, utilizing flavin adenine dinucleotide (FAD) and folate as essential cofactors for enzymatic activity. DMGDH is encoded by a gene located on human chromosome 5q14.1, and defects in this gene can lead to DMGDH deficiency, a condition characterized by symptoms such as muscle fatigue, a distinctive fish-like odor, and elevated levels of N,N-dimethylglycine in urine. DMGDH′s ability to facilitate the conversion of dimethylglycine is significant, as this process not only contributes to amino acid metabolism but also plays a role in the regulation of methylation processes within the body, highlighting DMGDH′s importance in overall metabolic health.

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DMGDH Antibody (C-9) References:

1. The purified recombinant precursor of rat mitochondrial dimethylglycine dehydrogenase binds FAD via an autocatalytic reaction.  |  Brizio, C., et al. 2008. Int J Biol Macromol. 42: 455-62. PMID: 18423846
2. Protection of cardiomyocytes from ischemic/hypoxic cell death via Drbp1 and pMe2GlyDH in cardio-specific ARC transgenic mice.  |  Pyo, JO., et al. 2008. J Biol Chem. 283: 30707-14. PMID: 18782777
3. Hepatocytes proteomic alteration and seroproteome analysis of HBV-transgenic mice.  |  Ding, C., et al. 2009. Proteomics. 9: 87-105. PMID: 19053081
4. Polymorphisms located in the region containing BHMT and BHMT2 genes as maternal protective factors for orofacial clefts.  |  Mostowska, A., et al. 2010. Eur J Oral Sci. 118: 325-32. PMID: 20662904
5. Identification of epistatic interactions through genome-wide association studies in sporadic medullary and juvenile papillary thyroid carcinomas.  |  Luzón-Toro, B., et al. 2015. BMC Med Genomics. 8: 83. PMID: 26690675
6. Structure and biochemical properties of recombinant human dimethylglycine dehydrogenase and comparison to the disease-related H109R variant.  |  Augustin, P., et al. 2016. FEBS J. 283: 3587-3603. PMID: 27486859
7. Sequence variation in folate pathway genes and risks of human cleft lip with or without cleft palate.  |  Marini, NJ., et al. 2016. Am J Med Genet A. 170: 2777-2787. PMID: 27604992
8. Copper and Zinc Levels, Prevalence of Common Variants of Genes Involved in Their Metabolism and Psoriasis Disease.  |  Dębniak, T., et al. 2025. Biomedicines. 13: PMID: 40002942
9. Covalent binding of folic acid to dimethylglycine dehydrogenase.  |  Wagner, C., et al. 1984. Arch Biochem Biophys. 233: 457-61. PMID: 6486795
10. Anti-mitochondrial antibodies in patients with dilated cardiomyopathy (anti-M7) are directed against flavoenzymes with covalently bound FAD.  |  Otto, A., et al. 1998. Clin Exp Immunol. 111: 541-7. PMID: 9528896

The preferred DMGDH antibody is DMGDH Antibody (E-6): sc-393178.