Dmc1 Antibody (F-2): sc-374364

Dmc1 Antibody (F-2) is a mouse monoclonal IgG1 antibody that detects Dmc1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Dmc1 is a crucial protein involved in meiotic recombination, a process essential for the accurate segregation of chromosomes during gamete formation. Dmc1 localizes to the nucleus, where anti-Dmc1 antibody (F-2) plays a pivotal role in facilitating the exchange of genetic material between homologous chromosomes, thereby ensuring genetic diversity and stability. Dmc1′s nuclear localization is vital, as Dmc1 interacts with other key proteins, such as Rad51, to form nucleoprotein filaments that are critical for the repair of DNA double-strand breaks. Furthermore, the alternative spliced form of Dmc1, known as Dmc1-D, is expressed in both male and female germ cells, suggesting a significant role in mammalian genetic recombination during meiosis. Dmc1 (F-2) antibody serves as an invaluable tool for researchers studying the mechanisms of DNA repair and recombination, as well as the implications of these processes in genetic disorders and cancer.

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Dmc1 Antibody (F-2) References:

1. Tid1/Rdh54 promotes colocalization of rad51 and dmc1 during meiotic recombination.  |  Shinohara, M., et al. 2000. Proc Natl Acad Sci U S A. 97: 10814-9. PMID: 11005857
2. Mei1 is epistatic to Dmc1 during mouse meiosis.  |  Reinholdt, LG. and Schimenti, JC. 2005. Chromosoma. 114: 127-34. PMID: 15928951
3. Tid1/Rdh54 promotes dissociation of Dmc1 from nonrecombinogenic sites on meiotic chromatin.  |  Holzen, TM., et al. 2006. Genes Dev. 20: 2593-604. PMID: 16980587
4. Interaction between Lim15/Dmc1 and the homologue of the large subunit of CAF-1: a molecular link between recombination and chromatin assembly during meiosis.  |  Ishii, S., et al. 2008. FEBS J. 275: 2032-41. PMID: 18355319
5. Genome wide cloning of maize meiotic recombinase Dmc1 and its functional structure through molecular phylogeny.  |  Etedali, F., et al. 2011. Genet Mol Res. 10: 1636-49. PMID: 21863556
6. Human PSF concentrates DNA and stimulates duplex capture in DMC1-mediated homologous pairing.  |  Morozumi, Y., et al. 2012. Nucleic Acids Res. 40: 3031-41. PMID: 22156371
7. Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis.  |  Cloud, V., et al. 2012. Science. 337: 1222-5. PMID: 22955832
8. In Vitro Assay for Plasmid Length DNA Strand Exchange by Human DMC1.  |  Goodson, SD., et al. 2019. Methods Mol Biol. 1999: 265-270. PMID: 31127583
9. A mutant form of Dmc1 that bypasses the requirement for accessory protein Mei5-Sae3 reveals independent activities of Mei5-Sae3 and Rad51 in Dmc1 filament stability.  |  Reitz, D., et al. 2019. PLoS Genet. 15: e1008217. PMID: 31790385
10. A pathogenic DMC1 frameshift mutation causes nonobstructive azoospermia but not primary ovarian insufficiency in humans.  |  Cao, D., et al. 2021. Mol Hum Reprod. 27: PMID: 34515795

The preferred Dmc1 antibody is Dmc1 Antibody (A-6): sc-373862.