Dmc1 Antibody (A-6): sc-373862
- Dmc1 Antibody (A-6) is a mouse monoclonal IgG1 λ Dmc1 antibody, cited in 6 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 67-99 within an internal region of Dmc1 of human origin
- Dmc1 Antibody (A-6) is recommended for detection of Dmc1 and Dmc1-D of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
- Anti-Dmc1 Antibody (A-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Dmc1 Antibody (A-6) for WB applications. This reagent is now offered in a bundle with Dmc1 Antibody (A-6) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Dmc1 Antibody (A-6) is a mouse monoclonal IgG1 antibody that detects Dmc1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Dmc1 antibody (A-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Dmc1 plays a crucial role in meiotic recombination, a process essential for accurate chromosome segregation during gamete formation. Specifically, Dmc1 facilitates homology search and strand invasion, vital steps in repairing double-strand breaks in DNA. This function ensures genetic diversity and stability in resulting gametes, contributing to overall organism health. Dmc1 interacts with other key proteins in the recombination pathway, such as Rad51 and Rad52, which are critical for maintaining genomic integrity. Dmc1 expression is especially significant in germ cells, where an alternative spliced form, Dmc1-D, has been identified, suggesting a nuanced role in mammalian genetic recombination during meiosis. Using Dmc1 (A-6) antibody in research can provide valuable insights into DNA repair mechanisms and their implications in genetic stability and disease.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Dmc1 Antibody (A-6) References:
- Hop2/Mnd1 acts on two critical steps in Dmc1-promoted homologous pairing. | Pezza, RJ., et al. 2007. Genes Dev. 21: 1758-66. PMID: 17639081
- Biochemical analysis of the human DMC1-I37N polymorphism. | Hikiba, J., et al. 2009. FEBS J. 276: 457-65. PMID: 19076215
- The Swi5-Sfr1 complex regulates Dmc1- and Rad51-driven DNA strand exchange proceeding through two distinct three-stranded intermediates by different mechanisms. | Ito, K., et al. 2024. Nucleic Acids Res. 52: 12517-12533. PMID: 39340300
- Cloning of human and mouse genes homologous to RAD52, a yeast gene involved in DNA repair and recombination. | Muris, DF., et al. 1994. Mutat Res. 315: 295-305. PMID: 7526206
- Expression of human RAD52 confers resistance to ionizing radiation in mammalian cells. | Park, MS. 1995. J Biol Chem. 270: 15467-70. PMID: 7797537
- A mouse homolog of the Escherichia coli recA and Saccharomyces cerevisiae RAD51 genes. | Morita, T., et al. 1993. Proc Natl Acad Sci U S A. 90: 6577-80. PMID: 8341671
- Specific interactions between the human RAD51 and RAD52 proteins. | Shen, Z., et al. 1996. J Biol Chem. 271: 148-52. PMID: 8550550
- A mutation in mouse rad51 results in an early embryonic lethal that is suppressed by a mutation in p53. | Lim, DS. and Hasty, P. 1996. Mol Cell Biol. 16: 7133-43. PMID: 8943369
- Nuclear import of DNA repair proteins. | Boulikas, T. 1997. Anticancer Res. 17: 843-63. PMID: 9137418
- Synergistic actions of Rad51 and Rad52 in recombination and DNA repair. | Benson, FE., et al. 1998. Nature. 391: 401-4. PMID: 9450758
- The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response. | Carney, JP., et al. 1998. Cell. 93: 477-86. PMID: 9590181
- The mouse RecA-like gene Dmc1 is required for homologous chromosome synapsis during meiosis. | Yoshida, K., et al. 1998. Mol Cell. 1: 707-18. PMID: 9660954
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dmc1 Antibody (A-6) | sc-373862 | 200 µg/ml | $322.00 | |||
Dmc1 Antibody (A-6): m-IgGλ BP-HRP Bundle | sc-522533 | 200 µg Ab, 40 µg BP | $361.00 | |||
Dmc1 Antibody (A-6) AC | sc-373862 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Dmc1 Antibody (A-6) HRP | sc-373862 HRP | 200 µg/ml | $322.00 | |||
Dmc1 Antibody (A-6) FITC | sc-373862 FITC | 200 µg/ml | $336.00 | |||
Dmc1 Antibody (A-6) PE | sc-373862 PE | 200 µg/ml | $349.00 | |||
Dmc1 Antibody (A-6) Alexa Fluor® 488 | sc-373862 AF488 | 200 µg/ml | $364.00 | |||
Dmc1 Antibody (A-6) Alexa Fluor® 546 | sc-373862 AF546 | 200 µg/ml | $364.00 | |||
Dmc1 Antibody (A-6) Alexa Fluor® 594 | sc-373862 AF594 | 200 µg/ml | $364.00 | |||
Dmc1 Antibody (A-6) Alexa Fluor® 647 | sc-373862 AF647 | 200 µg/ml | $364.00 | |||
Dmc1 Antibody (A-6) Alexa Fluor® 680 | sc-373862 AF680 | 200 µg/ml | $364.00 | |||
Dmc1 Antibody (A-6) Alexa Fluor® 790 | sc-373862 AF790 | 200 µg/ml | $364.00 | |||
Dmc1 (A-6) Neutralizing Peptide | sc-373862 P | 100 µg/0.5 ml | $69.00 |