Dmc1 Antibody (E-6): sc-365747
- Dmc1 Antibody (E-6) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- raised against amino acids 1-100 of Dmc1 of human origin
- recommended for detection of Dmc1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including bovine
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Dmc1 Antibody (E-6) for WB applications. These reagents are now offered in bundles with Dmc1 Antibody (E-6) (see ordering information below).
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Dmc1 Antibody (E-6) is a mouse monoclonal IgG2b light chain antibody that detects Dmc1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Dmc1 plays a crucial role in meiotic recombination, a process essential for the accurate segregation of chromosomes during gamete formation. Dmc1 is specifically localized to the nucleus, where Dmc1 facilitates the repair of DNA double-strand breaks by promoting homologous pairing and strand exchange, thereby ensuring genetic diversity and stability in offspring. Nuclear localization of Dmc1 is vital for meiotic process function; any disruption in Dmc1 activity can lead to chromosomal abnormalities and infertility. Additionally, Dmc1 interacts with other key proteins involved in recombination, such as Rad51, which further underscores Dmc1′s significance in the DNA repair pathway. anti-Dmc1 antibody (E-6) is an invaluable tool for researchers studying the mechanisms of genetic recombination and the implications of Dmc1 in reproductive biology and related disorders.
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Dmc1 Antibody (E-6) References:
- Identification of DMC1, a novel gene in the TOC region on 17q25.1 that shows loss of expression in multiple human cancers. | Harada, H., et al. 2001. J Hum Genet. 46: 90-5. PMID: 11281419
- Rad51-independent interchromosomal double-strand break repair by gene conversion requires Rad52 but not Rad55, Rad57, or Dmc1. | Pohl, TJ. and Nickoloff, JA. 2008. Mol Cell Biol. 28: 897-906. PMID: 18039855
- Structural and functional analyses of the DMC1-M200V polymorphism found in the human population. | Hikiba, J., et al. 2008. Nucleic Acids Res. 36: 4181-90. PMID: 18566005
- Dual regulation of Dmc1-driven DNA strand exchange by Swi5-Sfr1 activation and Rad22 inhibition. | Murayama, Y., et al. 2013. Genes Dev. 27: 2299-304. PMID: 24186976
- Exon skipping creates novel splice variants of DMC1 gene in ruminants. | Ahlawat, S., et al. 2016. Mol Cell Probes. 30: 66-73. PMID: 26945774
- Hop2 and Sae3 Are Required for Dmc1-Mediated Double-Strand Break Repair via Homolog Bias during Meiosis. | Cho, HR., et al. 2016. Mol Cells. 39: 550-6. PMID: 27329041
- Purification of Saccharomyces cerevisiae Homologous Recombination Proteins Dmc1 and Rdh54/Tid1 and a Fluorescent D-Loop Assay. | Chan, YL. and Bishop, DK. 2018. Methods Enzymol. 600: 307-320. PMID: 29458764
- The role of conserved amino acid residues of Sae3 in Mei5-Sae3 complex for Dmc1 assembly in meiotic recombination. | Sawant, P., et al. 2023. Genes Genet Syst. 98: 45-52. PMID: 37225456
- The Dmc1 recombinase physically interacts with and promotes the meiotic crossover functions of the Mlh1-Mlh3 endonuclease. | Pannafino, G., et al. 2024. Genetics. 227: PMID: 38657110
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dmc1 Antibody (E-6) | sc-365747 | 200 µg/ml | $322.00 | |||
Dmc1 Antibody (E-6): m-IgGκ BP-HRP Bundle | sc-535249 | 200 µg Ab; 40 µg BP | $361.00 | |||
Dmc1 Antibody (E-6): m-IgG2b BP-HRP Bundle | sc-549377 | 200 µg Ab; 10 µg BP | $361.00 |