Dmc1 Antibody (2H12/4): sc-53269
- Dmc1 Antibody (2H12/4) is a mouse monoclonal IgG2a κ, cited in 3 publications, provided at 200 µg/ml
- raised against full-length Dmc1 of human origin
- recommended for detection of Dmc1 of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Dmc1 Antibody (2H12/4) for WB and IHC(P) applications. These reagents are now offered in bundles with Dmc1 Antibody (2H12/4) (see ordering information below).
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Dmc1 Antibody (2H12/4) is a mouse monoclonal IgG2a antibody that detects Dmc1 in mouse, rat, human, and bovine samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Dmc1 plays a crucial role in meiotic recombination, a process essential for the accurate segregation of chromosomes during gamete formation. Dmc1 is primarily located in the nucleus, where Dmc1 facilitates the search for homologous sequences and promotes strand invasion, thereby ensuring genetic diversity and stability in offspring. Dmc1′s nuclear localization is vital for the formation of the nucleoprotein filament necessary for homologous pairing and recombination. Additionally, Dmc1 interacts with Rad51, another key protein in the double-strand-break repair pathway, highlighting Dmc1′s integral role in maintaining genomic integrity. Anti-Dmc1 antibody (2H12/4) serves as an invaluable tool for researchers studying the mechanisms of genetic recombination and the underlying causes of fertility issues or chromosomal disorders.
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Dmc1 Antibody (2H12/4) References:
- The Rad51 and Dmc1 recombinases: a non-identical twin relationship. | Masson, JY. and West, SC. 2001. Trends Biochem Sci. 26: 131-6. PMID: 11166572
- Roles of RecA homologues Rad51 and Dmc1 during meiotic recombination. | Shinohara, A. and Shinohara, M. 2004. Cytogenet Genome Res. 107: 201-7. PMID: 15467365
- From meiosis to postmeiotic events: uncovering the molecular roles of the meiosis-specific recombinase Dmc1. | Kagawa, W. and Kurumizaka, H. 2010. FEBS J. 277: 590-8. PMID: 20015079
- RAD51-associated protein 1 (RAD51AP1) interacts with the meiotic recombinase DMC1 through a conserved motif. | Dunlop, MH., et al. 2011. J Biol Chem. 286: 37328-34. PMID: 21903585
- BRCA2 regulates DMC1-mediated recombination through the BRC repeats. | Martinez, JS., et al. 2016. Proc Natl Acad Sci U S A. 113: 3515-20. PMID: 26976601
- Stabilization of the Human DMC1 Nucleoprotein Filament. | Waldvogel, SM., et al. 2019. Methods Mol Biol. 1999: 285-291. PMID: 31127585
- Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures. | Luo, SC., et al. 2021. Nat Commun. 12: 115. PMID: 33446654
- Hop2-Mnd1 and Swi5-Sfr1 stimulate Dmc1 filament assembly using distinct mechanisms. | Lee, W., et al. 2023. Nucleic Acids Res. 51: 8550-8562. PMID: 37395447
- DMC1 stabilizes crossovers at high and low temperatures during wheat meiosis. | Draeger, TN., et al. 2023. Front Plant Sci. 14: 1208285. PMID: 37615022
- BRCA2 stabilises RAD51 and DMC1 nucleoprotein filaments through a conserved interaction mode. | Dunce, JM. and Davies, OR. 2024. Nat Commun. 15: 8292. PMID: 39333100
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dmc1 Antibody (2H12/4) | sc-53269 | 200 µg/ml | $322.00 | |||
Dmc1 Antibody (2H12/4): m-IgG Fc BP-HRP Bundle | sc-536923 | 200 µg Ab; 10 µg BP | $361.00 | |||
Dmc1 Antibody (2H12/4): m-IgGκ BP-HRP Bundle | sc-534098 | 200 µg Ab; 40 µg BP | $361.00 |