DHCR24 Antibody (A-4): sc-398938
- DHCR24 Antibody (A-4) is a mouse monoclonal IgG2b κ DHCR24 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 145-166 near the N-terminus of DHCR24 of human origin
- DHCR24 Antibody (A-4) is recommended for detection of DHCR24 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-DHCR24 Antibody (A-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for DHCR24 Antibody (A-4) for WB and IHC(P) applications. These reagents are now offered in bundles with DHCR24 Antibody (A-4) (see ordering information below).
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DHCR24 Antibody (A-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects DHCR24 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-DHCR24 antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DHCR24 protein, also known as 3-β-hydroxysterol Delta-24 reductase or seladin-1, plays a crucial role in cholesterol biosynthesis by catalyzing the reduction of the Delta-24 double bond in sterol intermediates. This enzymatic activity is vital for maintaining cellular cholesterol levels, which are essential for membrane integrity, hormone synthesis, and overall cellular function. DHCR24 is predominantly expressed in the adrenal gland and brain, where DHCR24 is localized to the endoplasmic reticulum or Golgi apparatus. Notably, defects in the DHCR24 gene can lead to desmosterolosis, an autosomal recessive disorder characterized by abnormal sterol metabolism. DHCR24 (A-4) antibody′s ability to effectively detect this important protein makes DHCR24 (A-4) monoclonal antibody a valuable tool for researchers studying cholesterol metabolism and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
DHCR24 Antibody (A-4) References:
- Regulation of cellular response to oncogenic and oxidative stress by Seladin-1. | Wu, C., et al. 2004. Nature. 432: 640-5. PMID: 15577914
- DHCR24 gene expression is upregulated in melanoma metastases and associated to resistance to oxidative stress-induced apoptosis. | Di Stasi, D., et al. 2005. Int J Cancer. 115: 224-30. PMID: 15688385
- DHCR7 mutations in Brazilian Smith-Lemli-Opitz syndrome patients. | Scalco, FB., et al. 2005. Am J Med Genet A. 136: 278-81. PMID: 15952211
- Identification of nine novel DHCR7 missense mutations in patients with Smith-Lemli-Opitz syndrome (SLOS). | Waye, JS., et al. 2005. Hum Mutat. 26: 59. PMID: 15954111
- Seladin-1 as a target of estrogen receptor activation in the brain: a new gene for a rather old story? | Peri, A., et al. 2005. J Endocrinol Invest. 28: 285-93. PMID: 15954227
- Seladin-1/DHCR24 expression in normal ovary, ovarian epithelial and granulosa tumours. | Fuller, PJ., et al. 2005. Clin Endocrinol (Oxf). 63: 111-5. PMID: 15963070
- Smith-Lemli-Opitz syndrome in trisomy 13: how does the mix work? | Alkuraya, FS., et al. 2005. Birth Defects Res A Clin Mol Teratol. 73: 569-71. PMID: 15965973
- Molecular studies in Portuguese patients with Smith-Lemli-Opitz syndrome and report of three new mutations in DHCR7. | Cardoso, ML., et al. 2005. Mol Genet Metab. 85: 228-35. PMID: 15979035
- R352Q mutation of the DHCR7 gene is common among Japanese Smith-Lemli-Opitz syndrome patients. | Matsumoto, Y., et al. 2005. J Hum Genet. 50: 353-356. PMID: 16044199
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DHCR24 Antibody (A-4) | sc-398938 | 200 µg/ml | $322.00 | |||
DHCR24 Antibody (A-4): m-IgG Fc BP-HRP Bundle | sc-530982 | 200 µg Ab; 10 µg BP | $361.00 | |||
DHCR24 Antibody (A-4): m-IgGκ BP-HRP Bundle | sc-524549 | 200 µg Ab, 40 µg BP | $361.00 | |||
DHCR24 Antibody (A-4) AC | sc-398938 AC | 500 µg/ml, 25% agarose | $424.00 | |||
DHCR24 Antibody (A-4) HRP | sc-398938 HRP | 200 µg/ml | $322.00 | |||
DHCR24 Antibody (A-4) FITC | sc-398938 FITC | 200 µg/ml | $336.00 | |||
DHCR24 Antibody (A-4) PE | sc-398938 PE | 200 µg/ml | $349.00 | |||
DHCR24 Antibody (A-4) Alexa Fluor® 488 | sc-398938 AF488 | 200 µg/ml | $364.00 | |||
DHCR24 Antibody (A-4) Alexa Fluor® 546 | sc-398938 AF546 | 200 µg/ml | $364.00 | |||
DHCR24 Antibody (A-4) Alexa Fluor® 594 | sc-398938 AF594 | 200 µg/ml | $364.00 | |||
DHCR24 Antibody (A-4) Alexa Fluor® 647 | sc-398938 AF647 | 200 µg/ml | $364.00 | |||
DHCR24 Antibody (A-4) Alexa Fluor® 680 | sc-398938 AF680 | 200 µg/ml | $364.00 | |||
DHCR24 Antibody (A-4) Alexa Fluor® 790 | sc-398938 AF790 | 200 µg/ml | $364.00 | |||
DHCR24 (A-4) Neutralizing Peptide | sc-398938 P | 100 µg/0.5 ml | $69.00 |