DDB1 Antibody (E-11): sc-376860

DDB1 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects DDB1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-DDB1 antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDB1, part of the damaged DNA binding protein complex, plays a crucial role in the cellular response to DNA damage, particularly damage caused by ultraviolet light. DDB1 is essential for the recognition and repair of DNA lesions, specifically cyclobutane pyrimidine dimers, which are formed when DNA is exposed to UV radiation. DDB1′s ability to bind to these damaged sites is vital for initiating the nucleotide excision repair pathway, thereby preventing mutations that could lead to skin cancer and other genetic disorders. Mutations in the DDB2 gene, which encodes the other subunit of the DDB complex, are linked to xeroderma pigmentosum group E, a condition characterized by extreme sensitivity to sunlight and a predisposition to skin malignancies. Additionally, DDB1′s interaction with the hepatitis B virus X protein suggests a potential role in viral pathogenesis, highlighting DDB1′s importance not only in DNA repair but also in viral infections.

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DDB1 Antibody (E-11) References:

1. Studies of the murine DDB1 and DDB2 genes.  |  Zolezzi, F. and Linn, S. 2000. Gene. 245: 151-9. PMID: 10713455
2. Human damage-specific DNA-binding protein p48. Characterization of XPE mutations and regulation following UV irradiation.  |  Nichols, AF., et al. 2000. J Biol Chem. 275: 21422-8. PMID: 10777490
3. Dissociation of DDB1-binding and transactivation properties of the hepatitis B virus X protein.  |  Wentz, MJ., et al. 2000. Virus Res. 68: 87-92. PMID: 10930665
4. Identification of potential mRNA biomarkers in peripheral blood lymphocytes for human exposure to ionizing radiation.  |  Amundson, SA., et al. 2000. Radiat Res. 154: 342-6. PMID: 11012342
5. Damaged DNA-binding protein DDB stimulates the excision of cyclobutane pyrimidine dimers in vitro in concert with XPA and replication protein A.  |  Wakasugi, M., et al. 2001. J Biol Chem. 276: 15434-40. PMID: 11278856
6. The functions of the HIV1 protein Vpr and its action through the DCAF1.DDB1.Cullin4 ubiquitin ligase.  |  Casey, L., et al. 2010. Cytokine. 51: 1-9. PMID: 20347598
7. HBx affects CUL4-DDB1 function in both positive and negative manners.  |  Guo, L., et al. 2014. Biochem Biophys Res Commun. 450: 1492-7. PMID: 25019988
8. Expression of DDB1 is associated with subtypes of epithelial ovarian cancer and predicts clinical outcomes.  |  Shan, Y., et al. 2023. Tissue Cell. 82: 102072. PMID: 36934683
9. Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein.  |  Dualan, R., et al. 1995. Genomics. 29: 62-9. PMID: 8530102
10. Mutations specific to the xeroderma pigmentosum group E Ddb- phenotype.  |  Nichols, AF., et al. 1996. J Biol Chem. 271: 24317-20. PMID: 8798680
11. The V protein of the paramyxovirus SV5 interacts with damage-specific DNA binding protein.  |  Lin, GY., et al. 1998. Virology. 249: 189-200. PMID: 9740790
12. Refined mapping of the gene encoding the p127 kDa UV-damaged DNA-binding protein (DDB1) within 11q12-q13.1 and its exclusion in Best's vitelliform macular dystrophy.  |  Stöhr, H., et al. 1998. Eur J Hum Genet. 6: 400-5. PMID: 9781049

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDB1 Antibody (E-11) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-DDB1 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDB1 Antibody (E-11) Specifications

Alternate Product Name: DDB1

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 9-45

Epitope Target: DDB1

Epitope Species: human

Product Reactivity/Detection: DDB1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for DDB1 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin