DAP10 Antibody (C-1): sc-374038

DAP10 Antibody (C-1) is a mouse monoclonal IgG2a antibody that detects DAP10 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DAP10 is a transmembrane type 1 protein predominantly expressed in hematopoietic cells, and its function is crucial for immune response regulation. Anti-DAP10 antibody (C-1) forms an activating receptor complex with NKG2D, which is essential for initiating Natural Killer and T-cell mediated cytotoxicity against tumors that express ligands such as MICA and MICB. The interaction between DAP10 and NKG2D is particularly important in the context of epithelial tumors, where both proteins, along with their ligands, are stress-inducible molecules. Notably, the cytoplasmic region of DAP10 contains a binding site for the SH2 domain of the p85 subunit of phosphoinositide 3-kinase, indicating DAP10′s role as a signal transducer that activates PI 3-kinase, thereby influencing downstream signaling pathways that are vital for immune cell activation and function. DAP10 monoclonal antibody (C-1) is an invaluable tool for researchers studying immune responses and tumor biology, providing insights into the mechanisms of immune evasion and potential therapeutic targets.

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DAP10 Antibody (C-1) References:

1. Silencing human NKG2D, DAP10, and DAP12 reduces cytotoxicity of activated CD8+ T cells and NK cells.  |  Karimi, M., et al. 2005. J Immunol. 175: 7819-28. PMID: 16339517
2. IL-21 down-regulates NKG2D/DAP10 expression on human NK and CD8+ T cells.  |  Burgess, SJ., et al. 2006. J Immunol. 176: 1490-7. PMID: 16424177
3. Physiological roles of murine DAP10 adapter protein in tumor immunity and autoimmunity.  |  Hyka-Nouspikel, N. and Phillips, JH. 2006. Immunol Rev. 214: 106-17. PMID: 17100879
4. Cloning, sequencing, and cell surface expression pattern of bovine immunoreceptor NKG2D and adaptor molecules DAP10 and DAP12.  |  Fikri, Y., et al. 2007. Immunogenetics. 59: 653-9. PMID: 17530242
5. HMBOX1 negatively regulates NK cell functions by suppressing the NKG2D/DAP10 signaling pathway.  |  Wu, L., et al. 2011. Cell Mol Immunol. 8: 433-40. PMID: 21706044
6. A possible mechanism of impaired NK cytotoxicity in cancer patients: down-regulation of DAP10 by TGF-beta1.  |  Lee, JC., et al. 2011. Tumori. 97: 350-7. PMID: 21789015
7. Decreased expression of pSTAT, IRF-1 and DAP10 signalling molecules in peripheral blood lymphocytes of patients with metastatic melanoma.  |  Mirjačić Martinović, K., et al. 2016. J Clin Pathol. 69: 300-6. PMID: 26442832
8. TGF-β1 improving abnormal pregnancy outcomes induced by Toxoplasma gondii infection: Regulating NKG2D/DAP10 and killer subset of decidual NK cells.  |  Xu, X., et al. 2017. Cell Immunol. 317: 9-17. PMID: 28438315
9. A novel bispecific chimeric PD1-DAP10/NKG2D receptor augments NK92-cell therapy efficacy for human gastric cancer SGC-7901 cell.  |  Li, M., et al. 2020. Biochem Biophys Res Commun. 523: 745-752. PMID: 31952789
10. TCR extracellular domain genetically linked to CD28, 2B4/41BB and DAP10/CD3ζ -engineered NK cells mediates antitumor effects.  |  Li, S., et al. 2023. Cancer Immunol Immunother. 72: 769-774. PMID: 35988132

The preferred DAP10 antibody is DAP10 Antibody (H-2): sc-133173.