DAP10 Antibody (H-2): sc-133173

DAP10 Antibody (H-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects DAP10 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-DAP10 antibody (H-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DAP10 is a transmembrane type 1 protein predominantly expressed in hematopoietic cells, playing a crucial role in immune responses. Notably, DAP10 interacts with NKG2D receptor, forming a complex essential for initiating Natural Killer and T-cell mediated cytotoxicity against tumor cells expressing ligands such as MICA and MICB. This interaction enhances immune system response to stress-inducible molecules found in epithelial tumors, contributing to tumor surveillance. DAP10′s cytoplasmic region contains a binding site for SH2 domain of p85 subunit of phosphoinositide 3-kinase, indicating a role as a signal transducer that activates downstream signaling pathways. Such multifaceted functionality underscores DAP10′s significance in both immune regulation and potential therapeutic applications in cancer treatment.

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DAP10 Antibody (H-2) References:

1. Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA.  |  Bauer, S., et al. 1999. Science. 285: 727-9. PMID: 10426993
2. An activating immunoreceptor complex formed by NKG2D and DAP10.  |  Wu, J., et al. 1999. Science. 285: 730-2. PMID: 10426994
3. DAP12 and KAP10 (DAP10)-novel transmembrane adapter proteins of the CD3zeta family.  |  Wilson, MJ., et al. 2000. Immunol Res. 22: 21-42. PMID: 10945225
4. DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells.  |  Wu, J., et al. 2000. J Exp Med. 192: 1059-68. PMID: 11015446
5. Physiological roles of murine DAP10 adapter protein in tumor immunity and autoimmunity.  |  Hyka-Nouspikel, N. and Phillips, JH. 2006. Immunol Rev. 214: 106-17. PMID: 17100879
6. Regulation of human DAP10 gene expression in NK and T cells by Ap-1 transcription factors.  |  Marusina, AI., et al. 2008. J Immunol. 180: 409-17. PMID: 18097042
7. DAP10 contributes to CD8(+) T cell-mediated cytotoxic effector mechanisms during Mycobacterium tuberculosis infection.  |  Hessmann, M., et al. 2011. Immunobiology. 216: 639-47. PMID: 21122940
8. Complex regulation of human NKG2D-DAP10 cell surface expression: opposing roles of the γc cytokines and TGF-β1.  |  Park, YP., et al. 2011. Blood. 118: 3019-27. PMID: 21816829
9. A chimeric switch-receptor PD1-DAP10-41BB augments NK92-cell activation and killing for human lung Cancer H1299 Cell.  |  Zhi, L., et al. 2022. Biochem Biophys Res Commun. 600: 94-100. PMID: 35217362
10. SH2 domains recognize specific phosphopeptide sequences.  |  Songyang, Z., et al. 1993. Cell. 72: 767-78. PMID: 7680959
11. Cell stress-regulated human major histocompatibility complex class I gene expressed in gastrointestinal epithelium.  |  Groh, V., et al. 1996. Proc Natl Acad Sci U S A. 93: 12445-50. PMID: 8901601
12. Association of DAP12 with activating CD94/NKG2C NK cell receptors.  |  Lanier, LL., et al. 1998. Immunity. 8: 693-701. PMID: 9655483

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DAP10 Antibody (H-2) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-DAP10 Antibody (H-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DAP10 Antibody (H-2) Specifications

Alternate Product Name: DAP10

Product Clone Name: H-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-93

Epitope Target: DAP10

Epitope Species: human

Product Reactivity/Detection: DAP10

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for DAP10 Antibody (H-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin