D2HGDH Antibody (E-6): sc-514171

D2HGDH Antibody (E-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects D2HGDH protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-D2HGDH antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. D2HGDH, or D-2-hydroxyglutarate dehydrogenase, is a 521 amino acid enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 protein family, and plays a crucial role in cellular metabolism by catalyzing the conversion of D-2-hydroxyglutarate to alpha-ketoglutarate, a key intermediate in the tricarboxylic acid cycle. This enzymatic activity maintains the balance of metabolic pathways and energy production within the mitochondria, where D2HGDH is localized. D2HGDH′s importance is underscored by an association with D-2-hydroxyglutaric aciduria (D2HGA), a rare neurometabolic disorder arising from defects in the D2HGDH gene, leading to severe neurological symptoms such as early infantile-onset epileptic encephalopathy and cardiomyopathy. D2HGDH monoclonal antibody (E-6) serves as an essential tool for researchers studying D2HGDH′s implications in metabolic disorders and potential as a therapeutic target.

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D2HGDH Antibody (E-6) References:

1. Identification of a dehydrogenase acting on D-2-hydroxyglutarate.  |  Achouri, Y., et al. 2004. Biochem J. 381: 35-42. PMID: 15070399
2. Mutations in the D-2-hydroxyglutarate dehydrogenase gene cause D-2-hydroxyglutaric aciduria.  |  Struys, EA., et al. 2005. Am J Hum Genet. 76: 358-60. PMID: 15609246
3. Mutations in phenotypically mild D-2-hydroxyglutaric aciduria.  |  Struys, EA., et al. 2005. Ann Neurol. 58: 626-30. PMID: 16037974
4. Phenotypic heterogeneity in the presentation of D-2-hydroxyglutaric aciduria in monozygotic twins.  |  Misra, VK., et al. 2005. Mol Genet Metab. 86: 200-5. PMID: 16081310
5. D-2-hydroxyglutaric aciduria in three patients with proven SSADH deficiency: genetic coincidence or a related biochemical epiphenomenon?  |  Struys, EA., et al. 2006. Mol Genet Metab. 88: 53-7. PMID: 16442322
6. Measurement of D: -2-hydroxyglutarate dehydrogenase activity in cell homogenates derived from D: -2-hydroxyglutaric aciduria patients.  |  Wickenhagen, WV., et al. 2009. J Inherit Metab Dis. 32: 264-8. PMID: 19283509
7. Delineation of the intimate details of the backbone conformation of pyridine nucleotide coenzymes in aqueous solution.  |  Bose, KS. and Sarma, RH. 1975. Biochem Biophys Res Commun. 66: 1173-9. PMID: 2
8. D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2.  |  Lin, AP., et al. 2015. Nat Commun. 6: 7768. PMID: 26178471
9. Elevated d-2-hydroxyglutarate during colitis drives progression to colorectal cancer.  |  Han, J., et al. 2018. Proc Natl Acad Sci U S A. 115: 1057-1062. PMID: 29339485
10. MYC Regulation of D2HGDH and L2HGDH Influences the Epigenome and Epitranscriptome.  |  Qiu, Z., et al. 2020. Cell Chem Biol. 27: 538-550.e7. PMID: 32101699
11. D-2-hydroxyglutarate dehydrogenase in breast carcinoma as a potent prognostic marker associated with proliferation.  |  Hayashi, C., et al. 2021. Histol Histopathol. 36: 1053-1062. PMID: 34296423
12. D-2-hydroxyglutaric aciduria in a newborn with neurological abnormalities: a new neurometabolic disorder?  |  Gibson, KM., et al. 1993. J Inherit Metab Dis. 16: 497-500. PMID: 7609436

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-D2HGDH Antibody (E-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-D2HGDH Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

D2HGDH Antibody (E-6) Specifications

Alternate Product Name: D2HGDH

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 41-146

Epitope Target: D2HGDH

Epitope Species: human

Product Reactivity/Detection: D2HGDH

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for D2HGDH Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin